Optimized algorithm for Sanger sequencing-based EGFR mutation analyses in NSCLC biopsies
Pulmonary adenocarcinoma patients harboring EGFR mutations can benefit from tyrosine kinase inhibitor therapy. Reliable molecular analyses and precise pathological reporting of the EGFR mutational status are factors essential for patient treatment and outcome. More than 70 % of all EGFR mutation ana...
Gespeichert in:
| Hauptverfasser: | , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
15 March 2012
|
| In: |
Virchows Archiv
Year: 2012, Jahrgang: 460, Heft: 4, Pages: 407-414 |
| ISSN: | 1432-2307 |
| DOI: | 10.1007/s00428-012-1219-x |
| Online-Zugang: | Verlag, Volltext: http://dx.doi.org/10.1007/s00428-012-1219-x Verlag, Volltext: https://doi.org/10.1007/s00428-012-1219-x 
|
| Verfasserangaben: | Arne Warth, Roland Penzel, Regine Brandt, Christine Sers, Jürgen R. Fischer, Michael Thomas, Felix J. F. Herth, Manfred Dietel, Peter Schirmacher, Hendrik Bläker |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1581856563 | ||
| 003 | DE-627 | ||
| 005 | 20220815030824.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 181012s2012 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s00428-012-1219-x |2 doi | |
| 035 | |a (DE-627)1581856563 | ||
| 035 | |a (DE-576)511856563 | ||
| 035 | |a (DE-599)BSZ511856563 | ||
| 035 | |a (OCoLC)1341019991 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Warth, Arne |d 1979- |e VerfasserIn |0 (DE-588)132646145 |0 (DE-627)524716927 |0 (DE-576)260019755 |4 aut | |
| 245 | 1 | 0 | |a Optimized algorithm for Sanger sequencing-based EGFR mutation analyses in NSCLC biopsies |c Arne Warth, Roland Penzel, Regine Brandt, Christine Sers, Jürgen R. Fischer, Michael Thomas, Felix J. F. Herth, Manfred Dietel, Peter Schirmacher, Hendrik Bläker |
| 264 | 1 | |c 15 March 2012 | |
| 300 | |a 8 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 12.10.2018 | ||
| 520 | |a Pulmonary adenocarcinoma patients harboring EGFR mutations can benefit from tyrosine kinase inhibitor therapy. Reliable molecular analyses and precise pathological reporting of the EGFR mutational status are factors essential for patient treatment and outcome. More than 70 % of all EGFR mutation analyses are performed on non-small cell lung cancer (NSCLC) biopsies. However, biopsies may not be sufficient for mutation analysis due to low tumor content and admixture with non-neoplastic cells. To define the minimal concentration of tumor cells required for reliable EGFR mutational diagnostics by Sanger sequencing and to develop an algorithm for routine diagnostics on biopsy material, we determined total numbers of tumor and non-tumor cells, calculated the tumor cell concentration and serially diluted DNA from EGFR-mutated NSCLC by adding DNA of non-tumor cells from the same section. A counted tumor cell concentration of 30 %, which refers to a histologically estimated concentration of 40 %, is necessary for reliable detection of all mutations. Based on these data, we developed an algorithm for evidence-based EGFR mutation analysis by Sanger sequencing in biopsy specimens, which was subsequently applied to 461 diagnostic cases. Optimized diagnostic testing results in 80 % reliable EGFR mutation analyses of biopsy specimens, while in 20 % of cases re-biopsies had to be recommended. | ||
| 650 | 4 | |a Biopsy | |
| 650 | 4 | |a Detection limit | |
| 650 | 4 | |a EGFR | |
| 650 | 4 | |a Mutation analyses | |
| 650 | 4 | |a Non-small cell lung cancer | |
| 650 | 4 | |a Sanger sequencing | |
| 700 | 1 | |a Penzel, Roland |e VerfasserIn |0 (DE-588)102044021X |0 (DE-627)691221316 |0 (DE-576)360421083 |4 aut | |
| 700 | 1 | |a Brandt, Regine |e VerfasserIn |0 (DE-588)106314762X |0 (DE-627)81064696X |0 (DE-576)420525084 |4 aut | |
| 700 | 1 | |a Thomas, Michael |e VerfasserIn |0 (DE-588)1054438781 |0 (DE-627)79152213X |0 (DE-576)41027089X |4 aut | |
| 700 | 1 | |a Herth, Felix |e VerfasserIn |0 (DE-588)1016095236 |0 (DE-627)705477568 |0 (DE-576)351509925 |4 aut | |
| 700 | 1 | |a Schirmacher, Peter |d 1961- |e VerfasserIn |0 (DE-588)1020440112 |0 (DE-627)691221197 |0 (DE-576)360427448 |4 aut | |
| 700 | 1 | |a Bläker, Hendrik |d 1969- |e VerfasserIn |0 (DE-588)12093860X |0 (DE-627)08098150X |0 (DE-576)292457324 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Virchows Archiv |d Berlin : Springer, 1847 |g 460(2012), 4, Seite 407-414 |h Online-Ressource |w (DE-627)254910645 |w (DE-600)1463276-7 |w (DE-576)074754416 |x 1432-2307 |7 nnas |a Optimized algorithm for Sanger sequencing-based EGFR mutation analyses in NSCLC biopsies |
| 773 | 1 | 8 | |g volume:460 |g year:2012 |g number:4 |g pages:407-414 |g extent:8 |a Optimized algorithm for Sanger sequencing-based EGFR mutation analyses in NSCLC biopsies |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1007/s00428-012-1219-x |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00428-012-1219-x |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20181012 | ||
| 993 | |a Article | ||
| 994 | |a 2012 | ||
| 998 | |g 12093860X |a Bläker, Hendrik |m 12093860X:Bläker, Hendrik |d 910000 |d 912000 |e 910000PB12093860X |e 912000PB12093860X |k 0/910000/ |k 1/910000/912000/ |p 10 |y j | ||
| 998 | |g 1020440112 |a Schirmacher, Peter |m 1020440112:Schirmacher, Peter |d 910000 |d 912000 |d 700000 |d 718000 |e 910000PS1020440112 |e 912000PS1020440112 |e 700000PS1020440112 |e 718000PS1020440112 |k 0/910000/ |k 1/910000/912000/ |k 0/700000/ |k 1/700000/718000/ |p 9 | ||
| 998 | |g 1016095236 |a Herth, Felix |m 1016095236:Herth, Felix |d 910000 |d 950000 |d 950900 |e 910000PH1016095236 |e 950000PH1016095236 |e 950900PH1016095236 |k 0/910000/ |k 1/910000/950000/ |k 2/910000/950000/950900/ |p 7 | ||
| 998 | |g 1054438781 |a Thomas, Michael |m 1054438781:Thomas, Michael |d 910000 |d 950000 |d 950900 |e 910000PT1054438781 |e 950000PT1054438781 |e 950900PT1054438781 |k 0/910000/ |k 1/910000/950000/ |k 2/910000/950000/950900/ |p 6 | ||
| 998 | |g 106314762X |a Brandt, Regine |m 106314762X:Brandt, Regine |d 910000 |d 912000 |e 910000PB106314762X |e 912000PB106314762X |k 0/910000/ |k 1/910000/912000/ |p 3 | ||
| 998 | |g 102044021X |a Penzel, Roland |m 102044021X:Penzel, Roland |d 910000 |d 912000 |e 910000PP102044021X |e 912000PP102044021X |k 0/910000/ |k 1/910000/912000/ | ||
| 998 | |g 132646145 |a Warth, Arne |m 132646145:Warth, Arne |d 910000 |d 912000 |e 910000PW132646145 |e 912000PW132646145 |k 0/910000/ |k 1/910000/912000/ |p 1 |x j | ||
| 999 | |a KXP-PPN1581856563 |e 3028665603 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"person":[{"family":"Warth","role":"aut","given":"Arne","display":"Warth, Arne"},{"family":"Penzel","display":"Penzel, Roland","role":"aut","given":"Roland"},{"family":"Brandt","display":"Brandt, Regine","given":"Regine","role":"aut"},{"given":"Michael","role":"aut","display":"Thomas, Michael","family":"Thomas"},{"family":"Herth","role":"aut","given":"Felix","display":"Herth, Felix"},{"family":"Schirmacher","display":"Schirmacher, Peter","given":"Peter","role":"aut"},{"role":"aut","given":"Hendrik","display":"Bläker, Hendrik","family":"Bläker"}],"name":{"displayForm":["Arne Warth, Roland Penzel, Regine Brandt, Christine Sers, Jürgen R. Fischer, Michael Thomas, Felix J. F. Herth, Manfred Dietel, Peter Schirmacher, Hendrik Bläker"]},"physDesc":[{"extent":"8 S."}],"id":{"eki":["1581856563"],"doi":["10.1007/s00428-012-1219-x"]},"note":["Gesehen am 12.10.2018"],"relHost":[{"pubHistory":["1.1847 -"],"title":[{"title_sort":"Virchows Archiv","title":"Virchows Archiv","subtitle":"official journal of the European Society of Pathology"}],"part":{"pages":"407-414","extent":"8","issue":"4","volume":"460","text":"460(2012), 4, Seite 407-414","year":"2012"},"language":["eng"],"origin":[{"publisherPlace":"Berlin ; Heidelberg","dateIssuedKey":"1847","publisher":"Springer","dateIssuedDisp":"1847-"}],"recId":"254910645","titleAlt":[{"title":"Archiv für pathologische Anatomie und Physiologie und für klinische Medicin"},{"title":"Virchows Archiv für pathologische Anatomie und Physiologie und für klinische Medizin"},{"title":"Virchows Archiv / A"}],"type":{"media":"Online-Ressource","bibl":"periodical"},"disp":"Optimized algorithm for Sanger sequencing-based EGFR mutation analyses in NSCLC biopsiesVirchows Archiv","id":{"zdb":["1463276-7"],"issn":["1432-2307"],"eki":["254910645"]},"physDesc":[{"extent":"Online-Ressource"}],"note":["Gesehen am 06.12.05"],"corporate":[{"display":"European Society of Pathology","role":"isb"}]}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"origin":[{"dateIssuedDisp":"15 March 2012","dateIssuedKey":"2012"}],"recId":"1581856563","title":[{"title_sort":"Optimized algorithm for Sanger sequencing-based EGFR mutation analyses in NSCLC biopsies","title":"Optimized algorithm for Sanger sequencing-based EGFR mutation analyses in NSCLC biopsies"}],"language":["eng"]} | ||
| SRT | |a WARTHARNEPOPTIMIZEDA1520 | ||