Characterization of the African swine fever vrus decapping enzyme during infection
African swine fever virus (ASFV) infection is characterized by a progressive decrease in cellular protein synthesis with a concomitant increase in viral protein synthesis, though the mechanism by which the virus achieves this is still unknown. Decrease of cellular mRNA is observed during ASFV infect...
Gespeichert in:
| Hauptverfasser: | , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
11 October 2017
|
| In: |
Journal of virology
Year: 2017, Jahrgang: 91, Heft: 24, Pages: 1-19 |
| ISSN: | 1098-5514 |
| DOI: | 10.1128/JVI.00990-17 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1128/JVI.00990-17 Verlag, kostenfrei, Volltext: https://jvi.asm.org/content/91/24/e00990-17 
|
| Verfasserangaben: | Ana Quintas, Daniel Pérez-Núñez, Elena G. Sánchez, Maria L. Nogal, Matthias W. Hentze, Alfredo Castelló, Yolanda Revilla |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1581907575 | ||
| 003 | DE-627 | ||
| 005 | 20220815031536.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 181015s2017 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1128/JVI.00990-17 |2 doi | |
| 035 | |a (DE-627)1581907575 | ||
| 035 | |a (DE-576)511907575 | ||
| 035 | |a (DE-599)BSZ511907575 | ||
| 035 | |a (OCoLC)1341020044 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Quintas, Ana |e VerfasserIn |0 (DE-588)1169083986 |0 (DE-627)1032722754 |0 (DE-576)511909640 |4 aut | |
| 245 | 1 | 0 | |a Characterization of the African swine fever vrus decapping enzyme during infection |c Ana Quintas, Daniel Pérez-Núñez, Elena G. Sánchez, Maria L. Nogal, Matthias W. Hentze, Alfredo Castelló, Yolanda Revilla |
| 264 | 1 | |c 11 October 2017 | |
| 300 | |a 19 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 28.04.2022 | ||
| 520 | |a African swine fever virus (ASFV) infection is characterized by a progressive decrease in cellular protein synthesis with a concomitant increase in viral protein synthesis, though the mechanism by which the virus achieves this is still unknown. Decrease of cellular mRNA is observed during ASFV infection, suggesting that inhibition of cellular proteins is due to an active mRNA degradation process. ASFV carries a gene (Ba71V D250R/Malawi g5R) that encodes a decapping protein (ASFV-DP) that has a Nudix hydrolase motif and decapping activity in vitro. Here, we show that ASFV-DP was expressed from early times and accumulated throughout the infection with a subcellular localization typical of the endoplasmic reticulum, colocalizing with the cap structure and interacting with the ribosomal protein L23a. ASFV-DP was capable of interaction with poly(A) RNA in cultured cells, primarily mediated by the N-terminal region of the protein. ASFV-DP also interacted with viral and cellular RNAs in the context of infection, and its overexpression in infected cells resulted in decreased levels of both types of transcripts. This study points to ASFV-DP as a viral decapping enzyme involved in both the degradation of cellular mRNA and the regulation of viral transcripts. Importance: Virulent ASFV strains cause a highly infectious and lethal disease in domestic pigs for which there is no vaccine. Since 2007, an outbreak in the Caucasus region has spread to Russia, jeopardizing the European pig population and making it essential to deepen knowledge about the virus. Here, we demonstrate that ASFV-DP is a novel RNA-binding protein implicated in the regulation of mRNA metabolism during infection, making it a good target for vaccine development. | ||
| 700 | 1 | |a Hentze, Matthias W. |d 1960- |e VerfasserIn |0 (DE-588)143450395 |0 (DE-627)646106775 |0 (DE-576)337135754 |4 aut | |
| 700 | 1 | |a Castello, Alfredo |e VerfasserIn |0 (DE-588)116908687X |0 (DE-627)1032725222 |0 (DE-576)511912765 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of virology |d Baltimore, Md. : Soc., 1967 |g 91(2017), 24, Artikel-ID e00990-17, Seite 1-19 |h Online-Ressource |w (DE-627)303614609 |w (DE-600)1495529-5 |w (DE-576)08088766X |x 1098-5514 |7 nnas |a Characterization of the African swine fever vrus decapping enzyme during infection |
| 773 | 1 | 8 | |g volume:91 |g year:2017 |g number:24 |g elocationid:e00990-17 |g pages:1-19 |g extent:19 |a Characterization of the African swine fever vrus decapping enzyme during infection |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1128/JVI.00990-17 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u https://jvi.asm.org/content/91/24/e00990-17 |x Verlag |z kostenfrei |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20181015 | ||
| 993 | |a Article | ||
| 994 | |a 2017 | ||
| 998 | |g 143450395 |a Hentze, Matthias W. |m 143450395:Hentze, Matthias W. |d 50000 |d 700000 |d 718000 |e 50000PH143450395 |e 700000PH143450395 |e 718000PH143450395 |k 0/50000/ |k 0/700000/ |k 1/700000/718000/ |p 5 | ||
| 999 | |a KXP-PPN1581907575 |e 3028753677 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"recId":"1581907575","language":["eng"],"note":["Gesehen am 28.04.2022"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"person":[{"given":"Ana","family":"Quintas","role":"aut","roleDisplay":"VerfasserIn","display":"Quintas, Ana"},{"role":"aut","display":"Hentze, Matthias W.","roleDisplay":"VerfasserIn","given":"Matthias W.","family":"Hentze"},{"given":"Alfredo","family":"Castello","role":"aut","display":"Castello, Alfredo","roleDisplay":"VerfasserIn"}],"title":[{"title_sort":"Characterization of the African swine fever vrus decapping enzyme during infection","title":"Characterization of the African swine fever vrus decapping enzyme during infection"}],"relHost":[{"origin":[{"publisherPlace":"Baltimore, Md.","dateIssuedDisp":"1967-","publisher":"Soc.","dateIssuedKey":"1967"}],"id":{"issn":["1098-5514"],"zdb":["1495529-5"],"eki":["303614609"]},"physDesc":[{"extent":"Online-Ressource"}],"title":[{"subtitle":"publ. by the American Society for Microbiology","title":"Journal of virology","title_sort":"Journal of virology"}],"type":{"media":"Online-Ressource","bibl":"periodical"},"note":["Gesehen am 13.12.2021"],"disp":"Characterization of the African swine fever vrus decapping enzyme during infectionJournal of virology","recId":"303614609","corporate":[{"role":"isb","display":"American Society for Microbiology","roleDisplay":"Herausgebendes Organ"}],"language":["eng"],"pubHistory":["1.1967 -"],"part":{"issue":"24","pages":"1-19","year":"2017","extent":"19","volume":"91","text":"91(2017), 24, Artikel-ID e00990-17, Seite 1-19"}}],"physDesc":[{"extent":"19 S."}],"name":{"displayForm":["Ana Quintas, Daniel Pérez-Núñez, Elena G. Sánchez, Maria L. Nogal, Matthias W. Hentze, Alfredo Castelló, Yolanda Revilla"]},"id":{"doi":["10.1128/JVI.00990-17"],"eki":["1581907575"]},"origin":[{"dateIssuedDisp":"11 October 2017","dateIssuedKey":"2017"}]} | ||
| SRT | |a QUINTASANACHARACTERI1120 | ||