Accumulation of phosphatidylcholine on gut mucosal surface is not dominated by electrostatic interactions
The accumulation of phosphatidylcholine (PC) in the intestinal mucus layer is crucial for the protection of colon epithelia from the bacterial attack. It has been reported that the depletion of PC is a distinct feature of ulcerative colitis. Here we addressed the question how PC interacts with its b...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
15 February 2017
|
| In: |
Biochimica et biophysica acta. Biomembranes
Year: 2017, Jahrgang: 1859, Heft: 5, Pages: 959-965 |
| ISSN: | 1879-2642 |
| DOI: | 10.1016/j.bbamem.2017.02.008 |
| Online-Zugang: | Verlag, Volltext: http://dx.doi.org/10.1016/j.bbamem.2017.02.008 Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S0005273617300627 
|
| Verfasserangaben: | Agatha Korytowski, Wasim Abuillan, Federico Amadei, Ali Makky, Andrea Gumiero, Irmgard Sinning, Annika Gauss, Wolfgang Stremmel, Motomu Tanaka |
MARC
| LEADER | 00000caa a22000002c 4500 | ||
|---|---|---|---|
| 001 | 1582293937 | ||
| 003 | DE-627 | ||
| 005 | 20230427193203.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 181025s2017 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.bbamem.2017.02.008 |2 doi | |
| 035 | |a (DE-627)1582293937 | ||
| 035 | |a (DE-576)512293937 | ||
| 035 | |a (DE-599)BSZ512293937 | ||
| 035 | |a (OCoLC)1341021784 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 30 |2 sdnb | ||
| 100 | 1 | |a Korytowski, Agatha Anna |e VerfasserIn |0 (DE-588)109568616X |0 (DE-627)856321680 |0 (DE-576)467758085 |4 aut | |
| 245 | 1 | 0 | |a Accumulation of phosphatidylcholine on gut mucosal surface is not dominated by electrostatic interactions |c Agatha Korytowski, Wasim Abuillan, Federico Amadei, Ali Makky, Andrea Gumiero, Irmgard Sinning, Annika Gauss, Wolfgang Stremmel, Motomu Tanaka |
| 264 | 1 | |c 15 February 2017 | |
| 300 | |a 7 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Available online 15 February 2017 | ||
| 500 | |a Gesehen am 25.10.2018 | ||
| 520 | |a The accumulation of phosphatidylcholine (PC) in the intestinal mucus layer is crucial for the protection of colon epithelia from the bacterial attack. It has been reported that the depletion of PC is a distinct feature of ulcerative colitis. Here we addressed the question how PC interacts with its binding proteins, the mucins, which may establish the hydrophobic barrier against colonic microbiota. In the first step, the interactions of dioleoylphosphatidylcholine (DOPC) with two mucin preparations from porcine stomach, have been studied using dynamic light scattering, zeta potential measurement, and Langmuir isotherms, suggesting that mucin binds to the surface of DOPC vesicles. The enthalpy of mucin-PC interaction could be determined by isothermal titration calorimetry. The high affinity to PC found for both mucin types seems reasonable, as they mainly consist of mucin 2, a major constituent of the flowing mucus. Moreover, by the systematic variation of net charges, we concluded that the zwitterionic DOPC has the strongest binding affinity that cannot be explained within the electrostatic interactions between charged molecules. | ||
| 650 | 4 | |a Electrostatics | |
| 650 | 4 | |a Isothermal titration calorimetry | |
| 650 | 4 | |a Light scattering | |
| 650 | 4 | |a Lipid-protein interaction | |
| 650 | 4 | |a Mucin | |
| 650 | 4 | |a Ulcerative colitis | |
| 700 | 1 | |a Abuillan, Wasim |e VerfasserIn |0 (DE-588)1045296422 |0 (DE-627)774059095 |0 (DE-576)398923795 |4 aut | |
| 700 | 1 | |a Amadei, Federico |e VerfasserIn |0 (DE-588)1169856950 |0 (DE-627)103644967X |0 (DE-576)512290121 |4 aut | |
| 700 | 1 | |a Makky, Ali |e VerfasserIn |0 (DE-588)1169860737 |0 (DE-627)1036453359 |0 (DE-576)512293066 |4 aut | |
| 700 | 1 | |a Gumiero, Andrea |e VerfasserIn |0 (DE-588)1166462803 |0 (DE-627)1030437866 |0 (DE-576)510785107 |4 aut | |
| 700 | 1 | |a Sinning, Irmgard |d 1960- |e VerfasserIn |0 (DE-588)1027598617 |0 (DE-627)72945133X |0 (DE-576)166290580 |4 aut | |
| 700 | 1 | |a Gauss, Annika |d 1978- |e VerfasserIn |0 (DE-588)129331856 |0 (DE-627)393102092 |0 (DE-576)297603523 |4 aut | |
| 700 | 1 | |a Stremmel, Wolfgang |d 1952- |e VerfasserIn |0 (DE-588)142773638 |0 (DE-627)640118747 |0 (DE-576)33331560X |4 aut | |
| 700 | 1 | |a Tanaka, Motomu |e VerfasserIn |0 (DE-588)1060648377 |0 (DE-627)800414829 |0 (DE-576)416774636 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Biochimica et biophysica acta. Biomembranes |d Amsterdam : Elsevier, 1967 |g 1859(2017), 5, Seite 959-965 |w (DE-627)502923369 |w (DE-600)2209384-9 |w (DE-576)251822656 |x 1879-2642 |7 nnas |a Accumulation of phosphatidylcholine on gut mucosal surface is not dominated by electrostatic interactions |
| 773 | 1 | 8 | |g volume:1859 |g year:2017 |g number:5 |g pages:959-965 |g extent:7 |a Accumulation of phosphatidylcholine on gut mucosal surface is not dominated by electrostatic interactions |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.bbamem.2017.02.008 |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u http://www.sciencedirect.com/science/article/pii/S0005273617300627 |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20181025 | ||
| 993 | |a Article | ||
| 994 | |a 2017 | ||
| 998 | |g 1060648377 |a Tanaka, Motomu |m 1060648377:Tanaka, Motomu |d 120000 |d 120300 |e 120000PT1060648377 |e 120300PT1060648377 |k 0/120000/ |k 1/120000/120300/ |p 9 |y j | ||
| 998 | |g 142773638 |a Stremmel, Wolfgang |m 142773638:Stremmel, Wolfgang |d 910000 |d 910100 |e 910000PS142773638 |e 910100PS142773638 |k 0/910000/ |k 1/910000/910100/ |p 8 | ||
| 998 | |g 129331856 |a Gauss, Annika |m 129331856:Gauss, Annika |d 910000 |d 910100 |e 910000PG129331856 |e 910100PG129331856 |k 0/910000/ |k 1/910000/910100/ |p 7 | ||
| 998 | |g 1027598617 |a Sinning, Irmgard |m 1027598617:Sinning, Irmgard |d 700000 |d 711000 |e 700000PS1027598617 |e 711000PS1027598617 |k 0/700000/ |k 1/700000/711000/ |p 6 | ||
| 998 | |g 1166462803 |a Gumiero, Andrea |m 1166462803:Gumiero, Andrea |p 5 | ||
| 998 | |g 1169860737 |a Makky, Ali |m 1169860737:Makky, Ali |d 120000 |d 120300 |e 120000PM1169860737 |e 120300PM1169860737 |k 0/120000/ |k 1/120000/120300/ |p 4 | ||
| 998 | |g 1169856950 |a Amadei, Federico |m 1169856950:Amadei, Federico |d 120000 |d 120300 |e 120000PA1169856950 |e 120300PA1169856950 |k 0/120000/ |k 1/120000/120300/ |p 3 | ||
| 998 | |g 1045296422 |a Abuillan, Wasim |m 1045296422:Abuillan, Wasim |d 120000 |d 120300 |e 120000PA1045296422 |e 120300PA1045296422 |k 0/120000/ |k 1/120000/120300/ |p 2 | ||
| 998 | |g 109568616X |a Korytowski, Agatha Anna |m 109568616X:Korytowski, Agatha Anna |p 1 |x j | ||
| 999 | |a KXP-PPN1582293937 |e 3029690776 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"physDesc":[{"extent":"7 S."}],"id":{"doi":["10.1016/j.bbamem.2017.02.008"],"eki":["1582293937"]},"title":[{"title_sort":"Accumulation of phosphatidylcholine on gut mucosal surface is not dominated by electrostatic interactions","title":"Accumulation of phosphatidylcholine on gut mucosal surface is not dominated by electrostatic interactions"}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"origin":[{"dateIssuedKey":"2017","dateIssuedDisp":"15 February 2017"}],"relHost":[{"origin":[{"dateIssuedDisp":"1967-","publisher":"Elsevier","publisherPlace":"Amsterdam","dateIssuedKey":"1967"}],"title":[{"title":"Biochimica et biophysica acta","subtitle":"BBA","title_sort":"Biochimica et biophysica acta","partname":"Biomembranes"}],"id":{"eki":["502923369"],"issn":["1879-2642"],"zdb":["2209384-9"]},"recId":"502923369","titleAlt":[{"title":"Biochimica et biophysica acta / Biomembranes"}],"disp":"Accumulation of phosphatidylcholine on gut mucosal surface is not dominated by electrostatic interactionsBiochimica et biophysica acta. Biomembranes","type":{"media":"Online-Ressource","bibl":"periodical"},"note":["Gesehen am 08.01.2020","Bis 2016 Bände zugleich Bände von: Biochimica et biophysica acta"],"pubHistory":["135.1967 -"],"language":["eng"],"part":{"text":"1859(2017), 5, Seite 959-965","pages":"959-965","extent":"7","issue":"5","volume":"1859","year":"2017"}}],"language":["eng"],"name":{"displayForm":["Agatha Korytowski, Wasim Abuillan, Federico Amadei, Ali Makky, Andrea Gumiero, Irmgard Sinning, Annika Gauss, Wolfgang Stremmel, Motomu Tanaka"]},"note":["Available online 15 February 2017","Gesehen am 25.10.2018"],"recId":"1582293937","person":[{"family":"Korytowski","role":"aut","given":"Agatha Anna","display":"Korytowski, Agatha Anna"},{"display":"Abuillan, Wasim","given":"Wasim","role":"aut","family":"Abuillan"},{"given":"Federico","display":"Amadei, Federico","role":"aut","family":"Amadei"},{"family":"Makky","role":"aut","given":"Ali","display":"Makky, Ali"},{"family":"Gumiero","role":"aut","given":"Andrea","display":"Gumiero, Andrea"},{"given":"Irmgard","display":"Sinning, Irmgard","role":"aut","family":"Sinning"},{"role":"aut","family":"Gauss","given":"Annika","display":"Gauss, Annika"},{"role":"aut","family":"Stremmel","given":"Wolfgang","display":"Stremmel, Wolfgang"},{"family":"Tanaka","role":"aut","display":"Tanaka, Motomu","given":"Motomu"}]} | ||
| SRT | |a KORYTOWSKIACCUMULATI1520 | ||