Polyamine-modified near-infrared cyanine dyes for targeting the nuclei and nucleoli of cells
Near-infrared fluorescent probes have attracted great interest for bio-imaging applications since they allow for imaging with reduced background from cellular autofluorescence and deeper penetration depth than visible light. Herein, we present a library of 17 polyamine-modified near-infrared cyanine...
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| Hauptverfasser: | , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
October 2017
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| In: |
Dyes and pigments
Year: 2017, Jahrgang: 145, Pages: 80-94 |
| ISSN: | 0143-7208 |
| DOI: | 10.1016/j.dyepig.2017.05.041 |
| Online-Zugang: | Verlag, Volltext: http://dx.doi.org/10.1016/j.dyepig.2017.05.041 Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S0143720817302036 
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| Verfasserangaben: | Sandra G. König, Roland Krämer |
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| 520 | |a Near-infrared fluorescent probes have attracted great interest for bio-imaging applications since they allow for imaging with reduced background from cellular autofluorescence and deeper penetration depth than visible light. Herein, we present a library of 17 polyamine-modified near-infrared cyanine dyes differing in polyamine chain lengths or dye backbone. All dyes were characterized regarding their photophysical properties and show good to excellent fluorescence brightness values along with excellent solubility in water. The investigation of selective dye accumulation in cells allowed identification of a probe that selectively stains the nucleus in five different cell lines as well as probes that give promising results for the selective labeling of nucleoli. | ||
| 650 | 4 | |a Cell labeling | |
| 650 | 4 | |a Cyanines | |
| 650 | 4 | |a Fluorescence imaging | |
| 650 | 4 | |a Heptamethine dyes | |
| 650 | 4 | |a Indotricarbocyanines | |
| 650 | 4 | |a Near-infrared | |
| 650 | 4 | |a Nucleus staining | |
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