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Murine and human pancreatic tumor exosome recovery in mouse serum: diagnostic and prognostic potential and target cell delivery

Exosomes (Exo), powerful intercellular communicators, are recovered in all body fluids, suggesting suitability for diagnosis and prognosis. Easy in vitro manipulation recommends Exo as drug vehicles. Aiming to consolidate diagnostic and therapeutic potential of Exo, we evaluated recovery and fate of...

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Hauptverfasser: Erb, Ulrike (VerfasserIn) , Zhao, Kun (VerfasserIn) , Wang, Zhe (VerfasserIn) , Xiao, Li (VerfasserIn) , Zöller, Margot (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 10 September 2017
In: Cancer letters
Year: 2017, Jahrgang: 403, Pages: 1-12
ISSN:1872-7980
DOI:10.1016/j.canlet.2017.06.005
Online-Zugang:Verlag, Volltext: http://dx.doi.org/10.1016/j.canlet.2017.06.005
Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S0304383517303816
Volltext
Verfasserangaben:Ulrike Erb, Kun Zhao, Zhe Wang, Li Xiao, Margot Zöller
Beschreibung
Zusammenfassung:Exosomes (Exo), powerful intercellular communicators, are recovered in all body fluids, suggesting suitability for diagnosis and prognosis. Easy in vitro manipulation recommends Exo as drug vehicles. Aiming to consolidate diagnostic and therapeutic potential of Exo, we evaluated recovery and fate of tumor (TEX) and exogenous Exo in syngeneic and xenogeneic mice bearing a murine or a human pancreatic adenocarcinoma. A significant increase in serum (S)-TEX was observed 2 weeks after tumor cell application. Instead, S-TEX declined within 3-6 days after tumor excision. Intravenously injected dye-labeled TEX were rapidly cleared from the serum. Partly being degraded in the liver, the majority is taken-up by PBL, liver, bone marrow and lung cells. In the tumor-bearing host TEX persisted longer becoming enriched in tumor cells and metastatic organs. Accordingly, an antibody blockade of a TEX marker hampered disseminated tumor cell settlement in selected organs. In brief, a tumor marker panel appears suited for S-TEX recovery. In murine models, S-TEX are qualified for therapy control and follow-up studies. Despite rapid clearance from the serum, Exo uptake by host cells is most promising for tailored Exo as drug transporter.
Beschreibung:Available online 12 June 2017
Gesehen am 08.11.2018
Beschreibung:Online Resource
ISSN:1872-7980
DOI:10.1016/j.canlet.2017.06.005

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