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A high-resolution map of the human small non-coding transcriptome

Abstract Motivation. Although the amount of small non-coding RNA-sequencing data is continuously increasing, it is still unclear to which extent small RNAs are represented in the human genome. Results In this study we analyzed 303 billion sequencing reads from nearly 25 000 datasets to answer this q...

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Bibliographische Detailangaben
Hauptverfasser: Fehlmann, Tobias (VerfasserIn) , Haas, Jan (VerfasserIn) , Meder, Benjamin (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 2018
In: Bioinformatics
Year: 2017, Jahrgang: 34, Heft: 10, Pages: 1621-1628
ISSN:1367-4811
DOI:10.1093/bioinformatics/btx814
Online-Zugang:Verlag, Volltext: http://dx.doi.org/10.1093/bioinformatics/btx814
Verlag, Volltext: https://academic.oup.com/bioinformatics/article/34/10/1621/4769492
Volltext
Verfasserangaben:Tobias Fehlmann, Christina Backes, Julia Alles, Ulrike Fischer, Martin Hart, Fabian Kern, Hilde Langseth, Trine Rounge, Sinan Ugur Umu, Mustafa Kahraman, Thomas Laufer, Jan Haas, Cord Staehler, Nicole Ludwig, Matthias Hübenthal, Benjamin Meder, Andre Franke, Hans-Peter Lenhof, Eckart Meese and Andreas Keller
Beschreibung
Zusammenfassung:Abstract Motivation. Although the amount of small non-coding RNA-sequencing data is continuously increasing, it is still unclear to which extent small RNAs are represented in the human genome. Results In this study we analyzed 303 billion sequencing reads from nearly 25 000 datasets to answer this question. We determined that 0.8% of the human genome are reliably covered by 874 123 regions with an average length of 31 nt. On the basis of these regions, we found that among the known small non-coding RNA classes, microRNAs were the most prevalent. In subsequent steps, we characterized variations of miRNAs and performed a staged validation of 11 877 candidate miRNAs. Of these, many were actually expressed and significantly dysregulated in lung cancer. Selected candidates were finally validated by northern blots. Although isolated miRNAs could still be present in the human genome, our presented set likely contains the largest fraction of human miRNAs.
Beschreibung: Published: 21 December 2017
Gesehen am 14.11.2018
Beschreibung:Online Resource
ISSN:1367-4811
DOI:10.1093/bioinformatics/btx814

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