Cytochemical detection of peroxisomes in light and electron microscopy with 3,3′-diaminobenzidine
Peroxisomes are ubiquitous dynamic and multifunctional organelles that contribute to numerous anabolic and catabolic pathways, being essential for human health and development. Their best known functions include the oxidation of fatty acids and metabolism of hydrogen peroxide with catalase as a mark...
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| Dokumenttyp: | Kapitel/Artikel |
| Sprache: | Englisch |
| Veröffentlicht: |
14 April 2017
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| In: |
Peroxisomes
Year: 2017, Pages: 93-100 |
| DOI: | 10.1007/978-1-4939-6937-1_10 |
| Online-Zugang: | Verlag, Volltext: http://dx.doi.org/10.1007/978-1-4939-6937-1_10 Verlag, Volltext: https://doi.org/10.1007/978-1-4939-6937-1_10 
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| Verfasserangaben: | H. Dariush Fahimi |
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| 245 | 1 | 0 | |a Cytochemical detection of peroxisomes in light and electron microscopy with 3,3′-diaminobenzidine |c H. Dariush Fahimi |
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| 520 | |a Peroxisomes are ubiquitous dynamic and multifunctional organelles that contribute to numerous anabolic and catabolic pathways, being essential for human health and development. Their best known functions include the oxidation of fatty acids and metabolism of hydrogen peroxide with catalase as a marker enzyme. Indeed, historically, it was the cytochemical staining of catalase in many different cells and tissues that revealed the ubiquitous presence of peroxisomes in almost all animal and plant cells. In this chapter, the method for cytochemical staining of catalase with the alkaline 3, 3′-diaminobenzidine (DAB) is described. Since aldehyde fixation is a prerequisite for staining of catalase with DAB, a method for perfusion fixation of rat liver with glutaraldehyde is presented prior to the cytochemical staining method and the subsequent tissue processing for light and electron microscopy. | ||
| 650 | 4 | |a Catalase | |
| 650 | 4 | |a Cytochemistry | |
| 650 | 4 | |a DAB | |
| 650 | 4 | |a Diaminobenzidine | |
| 650 | 4 | |a Electron microscopy | |
| 650 | 4 | |a Histochemistry | |
| 650 | 4 | |a Light microscopy | |
| 650 | 4 | |a Perfusion fixation | |
| 650 | 4 | |a Peroxisome | |
| 773 | 0 | 8 | |i Enthalten in |t Peroxisomes |d New York, NY : Humana Press, 2017 |g (2017), Seite 93-100 |h Online-Ressource (XVII, 347 p. 63 illus., 36 illus. in color, online resource) |w (DE-627)1658175719 |w (DE-576)48887064X |z 9781493969371 |7 nnam |a Cytochemical detection of peroxisomes in light and electron microscopy with 3,3′-diaminobenzidine |
| 773 | 1 | 8 | |g year:2017 |g pages:93-100 |g extent:8 |a Cytochemical detection of peroxisomes in light and electron microscopy with 3,3′-diaminobenzidine |
| 776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |a Fahimi, H. Dariush, 1933 - |t Cytochemical detection of peroxisomes in light and electron microscopy with 3,3′-diaminobenzidine |d 2017 |w (DE-627)1583720901 |w (DE-576)513720901 |
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