Buchumschlag

Detailed characterization of early HIV-1 replication dynamics in primary human macrophages

Macrophages are natural target cells of human immunodeficiency virus type 1 (HIV-1). Viral replication appears to be delayed in these cells compared to lymphocytes; however, little is known about the kinetics of early post-entry events. Time-of-addition experiments using several HIV-1 inhibitors and...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Bejarano Acuña, David Alejandro (VerfasserIn) , Börner, Kathleen (VerfasserIn) , Müller, Barbara (VerfasserIn) , Kräusslich, Hans-Georg (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 10 November 2018
In: Viruses
Year: 2018, Jahrgang: 10, Heft: 11
ISSN:1999-4915
DOI:10.3390/v10110620
Online-Zugang:Verlag, kostenfrei, Volltext: http://dx.doi.org/10.3390/v10110620
Verlag, kostenfrei, Volltext: https://www.mdpi.com/1999-4915/10/11/620
Volltext
Verfasserangaben:David Alejandro Bejarano, Maria C. Puertas, Kathleen Börner, Javier Martinez-Picado, Barbara Müller and Hans-Georg Kräusslich

MARC

LEADER 00000caa a2200000 c 4500
001 1583895442
003 DE-627
005 20230427161322.0
007 cr uuu---uuuuu
008 181120s2018 xx |||||o 00| ||eng c
024 7 |a 10.3390/v10110620  |2 doi 
035 |a (DE-627)1583895442 
035 |a (DE-576)513895442 
035 |a (DE-599)BSZ513895442 
035 |a (OCoLC)1264010700 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 33  |2 sdnb 
100 1 |a Bejarano Acuña, David Alejandro  |e VerfasserIn  |0 (DE-588)1171866437  |0 (DE-627)1040752349  |0 (DE-576)513894535  |4 aut 
245 1 0 |a Detailed characterization of early HIV-1 replication dynamics in primary human macrophages  |c David Alejandro Bejarano, Maria C. Puertas, Kathleen Börner, Javier Martinez-Picado, Barbara Müller and Hans-Georg Kräusslich 
264 1 |c 10 November 2018 
300 |a 25 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Gesehen am 20.11.2018 
520 |a Macrophages are natural target cells of human immunodeficiency virus type 1 (HIV-1). Viral replication appears to be delayed in these cells compared to lymphocytes; however, little is known about the kinetics of early post-entry events. Time-of-addition experiments using several HIV-1 inhibitors and the detection of reverse transcriptase (RT) products with droplet digital PCR (ddPCR) revealed that early replication was delayed in primary human monocyte-derived macrophages of several donors and peaked late after infection. Direct imaging of reverse-transcription and pre-integration complexes (RTC/PIC) by click-labeling of newly synthesized DNA further confirmed our findings and showed a concomitant shift to the nuclear stage over time. Altering the entry pathway enhanced infectivity but did not affect kinetics of viral replication. The addition of viral protein X (Vpx) enhanced productive infection and accelerated completion of reverse transcription and nuclear entry. We propose that sterile alpha motif (SAM) and histidine/aspartate (HD) domain-containing protein 1 (SAMHD1) activity lowering deoxyribonucleotide triphosphate (dNTP) pools is the principal factor delaying early HIV-1 replication in macrophages. 
650 4 |a human immunodeficiency virus 
650 4 |a pre-integration complex 
650 4 |a primary macrophages 
650 4 |a replication kinetics 
650 4 |a reverse-transcription complex 
650 4 |a SAMHD1 
700 1 |a Börner, Kathleen  |e VerfasserIn  |0 (DE-588)1030276382  |0 (DE-627)734977727  |0 (DE-576)378068806  |4 aut 
700 1 |a Müller, Barbara  |d 1961-  |e VerfasserIn  |0 (DE-588)1028058667  |0 (DE-627)730238385  |0 (DE-576)375540024  |4 aut 
700 1 |a Kräusslich, Hans-Georg  |d 1958-  |e VerfasserIn  |0 (DE-588)1027601782  |0 (DE-627)729459004  |0 (DE-576)373450524  |4 aut 
773 0 8 |i Enthalten in  |t Viruses  |d Basel : MDPI, 2009  |g 10(2018,11) Artikel-Nummer 620, 25 Seiten  |h Online-Ressource  |w (DE-627)609775871  |w (DE-600)2516098-9  |w (DE-576)311326080  |x 1999-4915  |7 nnas  |a Detailed characterization of early HIV-1 replication dynamics in primary human macrophages 
773 1 8 |g volume:10  |g year:2018  |g number:11  |g extent:25  |a Detailed characterization of early HIV-1 replication dynamics in primary human macrophages 
856 4 0 |u http://dx.doi.org/10.3390/v10110620  |x Verlag  |x Resolving-System  |z kostenfrei  |3 Volltext 
856 4 0 |u https://www.mdpi.com/1999-4915/10/11/620  |x Verlag  |z kostenfrei  |3 Volltext 
951 |a AR 
992 |a 20181120 
993 |a Article 
994 |a 2018 
998 |g 1027601782  |a Kräusslich, Hans-Georg  |m 1027601782:Kräusslich, Hans-Georg  |d 910000  |d 911700  |e 910000PK1027601782  |e 911700PK1027601782  |k 0/910000/  |k 1/910000/911700/  |p 6  |y j 
998 |g 1028058667  |a Müller, Barbara  |m 1028058667:Müller, Barbara  |d 910000  |d 911700  |e 910000PM1028058667  |e 911700PM1028058667  |k 0/910000/  |k 1/910000/911700/  |p 5 
998 |g 1030276382  |a Börner, Kathleen  |m 1030276382:Börner, Kathleen  |d 910000  |d 911700  |e 910000PB1030276382  |e 911700PB1030276382  |k 0/910000/  |k 1/910000/911700/  |p 3 
998 |g 1171866437  |a Bejarano Acuña, David Alejandro  |m 1171866437:Bejarano Acuña, David Alejandro  |d 910000  |d 911700  |e 910000PB1171866437  |e 911700PB1171866437  |k 0/910000/  |k 1/910000/911700/  |p 1  |x j 
999 |a KXP-PPN1583895442  |e 3032811082 
BIB |a Y 
SER |a journal 
JSO |a {"person":[{"given":"David Alejandro","role":"aut","family":"Bejarano Acuña","display":"Bejarano Acuña, David Alejandro"},{"family":"Börner","display":"Börner, Kathleen","role":"aut","given":"Kathleen"},{"family":"Müller","display":"Müller, Barbara","role":"aut","given":"Barbara"},{"given":"Hans-Georg","role":"aut","family":"Kräusslich","display":"Kräusslich, Hans-Georg"}],"language":["eng"],"note":["Gesehen am 20.11.2018"],"origin":[{"dateIssuedDisp":"10 November 2018","dateIssuedKey":"2018"}],"title":[{"title_sort":"Detailed characterization of early HIV-1 replication dynamics in primary human macrophages","title":"Detailed characterization of early HIV-1 replication dynamics in primary human macrophages"}],"type":{"media":"Online-Ressource","bibl":"article-journal"},"relHost":[{"language":["eng"],"part":{"year":"2018","text":"10(2018,11) Artikel-Nummer 620, 25 Seiten","extent":"25","issue":"11","volume":"10"},"physDesc":[{"extent":"Online-Ressource"}],"name":{"displayForm":["Molecular Diversity Preservation International (MDPI)"]},"recId":"609775871","id":{"eki":["609775871"],"zdb":["2516098-9"],"issn":["1999-4915"]},"title":[{"title":"Viruses","title_sort":"Viruses"}],"origin":[{"dateIssuedKey":"2009","publisher":"MDPI","dateIssuedDisp":"2009-","publisherPlace":"Basel"}],"pubHistory":["1.2009 -"],"type":{"media":"Online-Ressource","bibl":"periodical"},"disp":"Detailed characterization of early HIV-1 replication dynamics in primary human macrophagesViruses"}],"recId":"1583895442","name":{"displayForm":["David Alejandro Bejarano, Maria C. Puertas, Kathleen Börner, Javier Martinez-Picado, Barbara Müller and Hans-Georg Kräusslich"]},"physDesc":[{"extent":"25 S."}],"id":{"doi":["10.3390/v10110620"],"eki":["1583895442"]}} 
SRT |a BEJARANOACDETAILEDCH1020 

Ähnliche Einträge