Cryopreservation of ovarian tissue: detailed description of methods for transport, freezing and thawing
Purpose: In many cases cancer therapy leads to an irreversible reduction or even loss of ovarian reserve. Cryopreservation of ovarian tissue with subsequent thawing and re-transplantation of tissue after the cancer is in remission constitutes a promising method to preserve fertility in women. To dat...
Gespeichert in:
| Hauptverfasser: | , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
7.8.2012
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| In: |
Geburtshilfe und Frauenheilkunde
Year: 2012, Jahrgang: 72, Heft: 10, Pages: 927-932 |
| ISSN: | 1438-8804 |
| DOI: | 10.1055/s-0032-1327812 |
| Online-Zugang: | Verlag, Volltext: http://dx.doi.org/10.1055/s-0032-1327812 Verlag, Volltext: http://www.thieme-connect.de/DOI/DOI?10.1055/s-0032-1327812 
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| Verfasserangaben: | V. Isachenko, R. Dittrich, G. Keck, E. Isachenko, G. Rahimi, H. van der Ven, M. Montag, I. Hoffmann, A. Müller, W. Distler, M.W. Beckmann, P. Mallmann |
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| 245 | 1 | 0 | |a Cryopreservation of ovarian tissue |b detailed description of methods for transport, freezing and thawing |c V. Isachenko, R. Dittrich, G. Keck, E. Isachenko, G. Rahimi, H. van der Ven, M. Montag, I. Hoffmann, A. Müller, W. Distler, M.W. Beckmann, P. Mallmann |
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| 520 | |a Purpose: In many cases cancer therapy leads to an irreversible reduction or even loss of ovarian reserve. Cryopreservation of ovarian tissue with subsequent thawing and re-transplantation of tissue after the cancer is in remission constitutes a promising method to preserve fertility in women. To date, more than 25 cases of live births after re-transplantation of cryopreserved ovarian tissue have been published worldwide. In Germany the first live birth after re-transplantation of cryopreserved tissue was in 2011. Material and Methods: After surgical removal of ovarian tissue in the Gynaecological Clinic of Dresden University, the tissue was sent to the Gynaecological Clinic of Bonn University in a special transport container at 5 °C and was frozen the next day using 1.5 M dimethyl sulfoxide cryosolution. In 2010 this ovarian tissue was thawed using a sucrose solution in the Gynaecological Clinic of Erlangen University Clinical Centre and was laparoscopically re-transplanted into the patient. Results: The patient became pregnant, the pregnancy was uneventful, and she gave birth to a healthy boy. Conclusion: Freezing of ovarian tissue with subsequent re-transplantation as described here is a viable method to preserve fertility in cancer patients. | ||
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