Systematic approaches to dissect biological processes in stem cells by image-based screening
High-throughput RNAi or small molecule screens have proven to be powerful methodologies for the systematic dissection of cellular processes. In model organisms and cell lines, large-scale screens have identified key components of many cellular pathways and helped to identify novel targets in disease...
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| Hauptverfasser: | , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
01 June 2012
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| In: |
Biotechnology journal
Year: 2012, Jahrgang: 7, Heft: 6, Pages: 768-778 |
| ISSN: | 1860-7314 |
| DOI: | 10.1002/biot.201200117 |
| Online-Zugang: | Verlag, Pay-per-use, Volltext: http://dx.doi.org/10.1002/biot.201200117 Verlag, Pay-per-use, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/biot.201200117 
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| Verfasserangaben: | Gerrit Erdmann, H. Christian Volz and Michael Boutros |
MARC
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| 520 | |a High-throughput RNAi or small molecule screens have proven to be powerful methodologies for the systematic dissection of cellular processes. In model organisms and cell lines, large-scale screens have identified key components of many cellular pathways and helped to identify novel targets in disease-relevant pathways. Image-based high-content screening has become an increasingly important tool in high-throughput screening, enabling changes in phenotype characteristics, such as cell morphology and cell differentiation, to be monitored. In this review, we discuss the use of image-based screening approaches to explore the behavior of adult, embryonic, and induced pluripotent stem cells. First, we review how current pluripotency and differentiation assays can be adapted to high-throughput formats. We then describe general aspects of image-based screening of cells and present an outlook on challenges for screening stem cells. | ||
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