Cover Image

Glutathione redox potential in the mitochondrial intermembrane space is linked to the cytosol and impacts the Mia40 redox state

Glutathione is an important mediator and regulator of cellular redox processes. Detailed knowledge of local glutathione redox potential (EGSH) dynamics is critical to understand the network of redox processes and their influence on cellular function. Using dynamic oxidant recovery assays together wi...

Full description

Saved in:
Bibliographic Details
Main Authors: Kojer, Kerstin (Author) , Bien, Melanie (Author) , Dick, Tobias P. (Author)
Format: Article (Journal)
Language:English
Published: 15.06.2012
In: The EMBO journal
Year: 2012, Volume: 31, Issue: 14, Pages: 3169-3182
ISSN:1460-2075
DOI:10.1038/emboj.2012.165
Online Access:Verlag, Volltext: http://dx.doi.org/10.1038/emboj.2012.165
Verlag, Volltext: http://emboj.embopress.org/content/31/14/3169
Get full text
Author Notes:Kerstin Kojer, Melanie Bien, Heike Gangel, Bruce Morgan, Tobias P. Dick, Jan Riemer

MARC

LEADER 00000caa a2200000 c 4500
001 1583974792
003 DE-627
005 20220815063434.0
007 cr uuu---uuuuu
008 181122s2012 xx |||||o 00| ||eng c
024 7 |a 10.1038/emboj.2012.165  |2 doi 
035 |a (DE-627)1583974792 
035 |a (DE-576)513974792 
035 |a (DE-599)BSZ513974792 
035 |a (OCoLC)1341024129 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 33  |2 sdnb 
100 1 |a Kojer, Kerstin  |d 1984-  |e VerfasserIn  |0 (DE-588)1043180176  |0 (DE-627)770196527  |0 (DE-576)394522818  |4 aut 
245 1 0 |a Glutathione redox potential in the mitochondrial intermembrane space is linked to the cytosol and impacts the Mia40 redox state  |c Kerstin Kojer, Melanie Bien, Heike Gangel, Bruce Morgan, Tobias P. Dick, Jan Riemer 
264 1 |c 15.06.2012 
300 |a 14 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Gesehen am 22.11.2018 
520 |a Glutathione is an important mediator and regulator of cellular redox processes. Detailed knowledge of local glutathione redox potential (EGSH) dynamics is critical to understand the network of redox processes and their influence on cellular function. Using dynamic oxidant recovery assays together with EGSH‐specific fluorescent reporters, we investigate the glutathione pools of the cytosol, mitochondrial matrix and intermembrane space (IMS). We demonstrate that the glutathione pools of IMS and cytosol are dynamically interconnected via porins. In contrast, no appreciable communication was observed between the glutathione pools of the IMS and matrix. By modulating redox pathways in the cytosol and IMS, we find that the cytosolic glutathione reductase system is the major determinant of EGSH in the IMS, thus explaining a steady‐state EGSH in the IMS which is similar to the cytosol. Moreover, we show that the local EGSH contributes to the partially reduced redox state of the IMS oxidoreductase Mia40 in vivo. Taken together, we provide a comprehensive mechanistic picture of the IMS redox milieu and define the redox influences on Mia40 in living cells. 
650 4 |a glutathione 
650 4 |a Mia40 
650 4 |a mitochondrial intermembrane space 
650 4 |a porin 
650 4 |a roGFP 
700 1 |a Bien, Melanie  |d 1978-  |e VerfasserIn  |0 (DE-588)136081592  |0 (DE-627)624913597  |0 (DE-576)322207029  |4 aut 
700 1 |a Dick, Tobias P.  |e VerfasserIn  |0 (DE-588)1142415805  |0 (DE-627)100217807X  |0 (DE-576)494813431  |4 aut 
773 0 8 |i Enthalten in  |a European Molecular Biology Organization  |t The EMBO journal  |d [London] : Nature Publishing Group UK, 1982  |g 31(2012), 14, Seite 3169-3182  |h Online-Ressource  |w (DE-627)266022529  |w (DE-600)1467419-1  |w (DE-576)075961377  |x 1460-2075  |7 nnas 
773 1 8 |g volume:31  |g year:2012  |g number:14  |g pages:3169-3182  |g extent:14  |a Glutathione redox potential in the mitochondrial intermembrane space is linked to the cytosol and impacts the Mia40 redox state 
856 4 0 |u http://dx.doi.org/10.1038/emboj.2012.165  |x Verlag  |x Resolving-System  |3 Volltext 
856 4 0 |u http://emboj.embopress.org/content/31/14/3169  |x Verlag  |3 Volltext 
951 |a AR 
992 |a 20181122 
993 |a Article 
994 |a 2012 
998 |g 1142415805  |a Dick, Tobias P.  |m 1142415805:Dick, Tobias P.  |d 140000  |d 700000  |d 718000  |e 140000PD1142415805  |e 700000PD1142415805  |e 718000PD1142415805  |k 0/140000/  |k 0/700000/  |k 1/700000/718000/  |p 5 
999 |a KXP-PPN1583974792  |e 3032927552 
BIB |a Y 
SER |a journal 
JSO |a {"title":[{"title_sort":"Glutathione redox potential in the mitochondrial intermembrane space is linked to the cytosol and impacts the Mia40 redox state","title":"Glutathione redox potential in the mitochondrial intermembrane space is linked to the cytosol and impacts the Mia40 redox state"}],"person":[{"family":"Kojer","given":"Kerstin","display":"Kojer, Kerstin","roleDisplay":"VerfasserIn","role":"aut"},{"roleDisplay":"VerfasserIn","display":"Bien, Melanie","role":"aut","family":"Bien","given":"Melanie"},{"display":"Dick, Tobias P.","roleDisplay":"VerfasserIn","role":"aut","family":"Dick","given":"Tobias P."}],"type":{"media":"Online-Ressource","bibl":"article-journal"},"note":["Gesehen am 22.11.2018"],"language":["eng"],"recId":"1583974792","origin":[{"dateIssuedDisp":"15.06.2012","dateIssuedKey":"2012"}],"id":{"doi":["10.1038/emboj.2012.165"],"eki":["1583974792"]},"name":{"displayForm":["Kerstin Kojer, Melanie Bien, Heike Gangel, Bruce Morgan, Tobias P. Dick, Jan Riemer"]},"physDesc":[{"extent":"14 S."}],"relHost":[{"title":[{"title":"The EMBO journal","title_sort":"EMBO journal"}],"pubHistory":["1.1982 -"],"titleAlt":[{"title":"The EMBO journal online"}],"part":{"year":"2012","issue":"14","pages":"3169-3182","volume":"31","text":"31(2012), 14, Seite 3169-3182","extent":"14"},"disp":"European Molecular Biology OrganizationThe EMBO journal","type":{"media":"Online-Ressource","bibl":"periodical"},"note":["Gesehen am 18.02.2016","Fortsetzung der Druckausgabe"],"corporate":[{"role":"aut","display":"European Molecular Biology Organization","roleDisplay":"VerfasserIn"}],"language":["eng"],"recId":"266022529","origin":[{"publisherPlace":"[London] ; Heidelberg ; Oxford [u.a.] ; London [u.a.] ; Hoboken, NJ [u.a.]","publisher":"Nature Publishing Group UK ; EMBO Press ; Oxford Univ. Press ; Nature Publ. Group ; Wiley","dateIssuedKey":"1982","dateIssuedDisp":"1982-"}],"id":{"eki":["266022529"],"doi":["10.1002/(ISSN)1460-2075"],"zdb":["1467419-1"],"issn":["1460-2075"]},"name":{"displayForm":["publ. for the European Molecular Biology Organization"]},"physDesc":[{"extent":"Online-Ressource"}]}]} 
SRT |a KOJERKERSTGLUTATHION1506 

Similar Items