Bivalent ligand UDCA-LPE inhibits pro-fibrogenic integrin signalling by inducing lipid raft-mediated internalization
Ursodeoxycholyl lysophosphatidylethanolamide (UDCA-LPE) is a synthetic bile acid-phospholipid conjugate with profound hepatoprotective and anti-fibrogenic functions in vitro and in vivo. Herein, we aimed to demonstrate the inhibitory effects of UDCA-LPE on pro-fibrogenic integrin signalling. UDCA-LP...
Gespeichert in:
| Hauptverfasser: | , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
20 October 2018
|
| In: |
International journal of molecular sciences
Year: 2018, Jahrgang: 19, Heft: 10, Pages: 1-15 |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms19103254 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: http://dx.doi.org/10.3390/ijms19103254 Verlag, kostenfrei, Volltext: https://www.mdpi.com/1422-0067/19/10/3254 
|
| Verfasserangaben: | Jie Su, Hongying Gan-Schreier, Benjamin Goeppert, Walee Chamulitrat, Wolfgang Stremmel and Anita Pathil |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1584190817 | ||
| 003 | DE-627 | ||
| 005 | 20220330113053.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 181123s2018 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.3390/ijms19103254 |2 doi | |
| 035 | |a (DE-627)1584190817 | ||
| 035 | |a (DE-576)514190817 | ||
| 035 | |a (DE-599)BSZ514190817 | ||
| 035 | |a (OCoLC)1264010834 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Su, Jie |d 1986- |e VerfasserIn |0 (DE-588)1133222072 |0 (DE-627)888962061 |0 (DE-576)48919057X |4 aut | |
| 245 | 1 | 0 | |a Bivalent ligand UDCA-LPE inhibits pro-fibrogenic integrin signalling by inducing lipid raft-mediated internalization |c Jie Su, Hongying Gan-Schreier, Benjamin Goeppert, Walee Chamulitrat, Wolfgang Stremmel and Anita Pathil |
| 264 | 1 | |c 20 October 2018 | |
| 300 | |a 15 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 520 | |a Ursodeoxycholyl lysophosphatidylethanolamide (UDCA-LPE) is a synthetic bile acid-phospholipid conjugate with profound hepatoprotective and anti-fibrogenic functions in vitro and in vivo. Herein, we aimed to demonstrate the inhibitory effects of UDCA-LPE on pro-fibrogenic integrin signalling. UDCA-LPE treatment of human embryonic liver cell line CL48 and primary human hepatic stellate cells induced a non-classical internalization of integrin β1 resulting in dephosphorylation and inhibition of SRC and focal adhesion kinase (FAK). Signalling analyses suggested that UDCA-LPE may act as a heterobivalent ligand for integrins and lysophospholipid receptor1 (LPAR1) and co-immunoprecipitation demonstrated the bridging effect of UDCA-LPE on integrin β1 and LPAR1. The disruption of either the UDCA-moiety binding to integrins by RGD-containing peptide GRGDSP or the LPE-moiety binding to LPAR1 by LPAR1 antagonist Ki16425 reversed inhibitory functions of UDCA-LPE. The lack of inhibitory functions of UDCA-PE and UDCA-LPE derivatives (14:0 and 12:0, LPE-moiety containing shorter fatty acid chain) as well as the consistency of the translocation of UDCA-LPE and integrins, which co-fractionated with LPE but not UDCA, suggested that the observed UDCA-LPE-induced translocation of integrins was mediated by LPE endocytic transport pathway. | ||
| 650 | 4 | |a hepatic fibrosis | |
| 650 | 4 | |a integrin signalling | |
| 650 | 4 | |a lipid raft-mediated internalization | |
| 700 | 1 | |a Gan-Schreier, Hongying |d 1967- |e VerfasserIn |0 (DE-588)130102040 |0 (DE-627)490859860 |0 (DE-576)297997955 |4 aut | |
| 700 | 1 | |a Goeppert, Benjamin |d 1977- |e VerfasserIn |0 (DE-588)1022581635 |0 (DE-627)717004244 |0 (DE-576)365641324 |4 aut | |
| 700 | 1 | |a Chamulitrat, Walee |d 1959- |e VerfasserIn |0 (DE-588)1037336828 |0 (DE-627)755493915 |0 (DE-576)39134479X |4 aut | |
| 700 | 1 | |a Stremmel, Wolfgang |d 1952- |e VerfasserIn |0 (DE-588)142773638 |0 (DE-627)640118747 |0 (DE-576)33331560X |4 aut | |
| 700 | 1 | |a Pathil-Warth, Anita |d 1981- |e VerfasserIn |0 (DE-588)132644304 |0 (DE-627)524715890 |0 (DE-576)260118915 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t International journal of molecular sciences |d Basel : Molecular Diversity Preservation International, 2000 |g 19(2018), 10, Artikel-ID 3254, Seite 1-15 |h Online-Ressource |w (DE-627)316340715 |w (DE-600)2019364-6 |w (DE-576)281194653 |x 1422-0067 |7 nnas |a Bivalent ligand UDCA-LPE inhibits pro-fibrogenic integrin signalling by inducing lipid raft-mediated internalization |
| 773 | 1 | 8 | |g volume:19 |g year:2018 |g number:10 |g elocationid:3254 |g pages:1-15 |g extent:15 |a Bivalent ligand UDCA-LPE inhibits pro-fibrogenic integrin signalling by inducing lipid raft-mediated internalization |
| 856 | 4 | 0 | |u http://dx.doi.org/10.3390/ijms19103254 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u https://www.mdpi.com/1422-0067/19/10/3254 |x Verlag |z kostenfrei |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20181123 | ||
| 993 | |a Article | ||
| 994 | |a 2018 | ||
| 998 | |g 132644304 |a Pathil-Warth, Anita |m 132644304:Pathil-Warth, Anita |d 910000 |d 910100 |e 910000PP132644304 |e 910100PP132644304 |k 0/910000/ |k 1/910000/910100/ |p 6 |y j | ||
| 998 | |g 142773638 |a Stremmel, Wolfgang |m 142773638:Stremmel, Wolfgang |d 910000 |d 910100 |e 910000PS142773638 |e 910100PS142773638 |k 0/910000/ |k 1/910000/910100/ |p 5 | ||
| 998 | |g 1037336828 |a Chamulitrat, Walee |m 1037336828:Chamulitrat, Walee |d 910000 |d 910100 |e 910000PC1037336828 |e 910100PC1037336828 |k 0/910000/ |k 1/910000/910100/ |p 4 | ||
| 998 | |g 1022581635 |a Goeppert, Benjamin |m 1022581635:Goeppert, Benjamin |d 910000 |d 912000 |e 910000PG1022581635 |e 912000PG1022581635 |k 0/910000/ |k 1/910000/912000/ |p 3 | ||
| 998 | |g 130102040 |a Gan-Schreier, Hongying |m 130102040:Gan-Schreier, Hongying |d 910000 |d 910100 |e 910000PG130102040 |e 910100PG130102040 |k 0/910000/ |k 1/910000/910100/ |p 2 | ||
| 999 | |a KXP-PPN1584190817 |e 3033382886 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"relHost":[{"part":{"volume":"19","extent":"15","pages":"1-15","text":"19(2018), 10, Artikel-ID 3254, Seite 1-15","year":"2018","issue":"10"},"type":{"media":"Online-Ressource","bibl":"periodical"},"note":["Gesehen am 17.09.20"],"origin":[{"dateIssuedKey":"2000","publisher":"Molecular Diversity Preservation International","publisherPlace":"Basel","dateIssuedDisp":"2000-"}],"titleAlt":[{"title":"IJMS"}],"title":[{"title":"International journal of molecular sciences","title_sort":"International journal of molecular sciences"}],"language":["eng"],"disp":"Bivalent ligand UDCA-LPE inhibits pro-fibrogenic integrin signalling by inducing lipid raft-mediated internalizationInternational journal of molecular sciences","id":{"zdb":["2019364-6"],"eki":["316340715"],"issn":["1422-0067","1661-6596"]},"physDesc":[{"extent":"Online-Ressource"}],"recId":"316340715","pubHistory":["1.2000 -"]}],"id":{"doi":["10.3390/ijms19103254"],"eki":["1584190817"]},"name":{"displayForm":["Jie Su, Hongying Gan-Schreier, Benjamin Goeppert, Walee Chamulitrat, Wolfgang Stremmel and Anita Pathil"]},"physDesc":[{"extent":"15 S."}],"recId":"1584190817","origin":[{"dateIssuedDisp":"20 October 2018","dateIssuedKey":"2018"}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"title":[{"title":"Bivalent ligand UDCA-LPE inhibits pro-fibrogenic integrin signalling by inducing lipid raft-mediated internalization","title_sort":"Bivalent ligand UDCA-LPE inhibits pro-fibrogenic integrin signalling by inducing lipid raft-mediated internalization"}],"language":["eng"],"person":[{"role":"aut","given":"Jie","display":"Su, Jie","family":"Su"},{"given":"Hongying","role":"aut","display":"Gan-Schreier, Hongying","family":"Gan-Schreier"},{"role":"aut","given":"Benjamin","family":"Goeppert","display":"Goeppert, Benjamin"},{"display":"Chamulitrat, Walee","family":"Chamulitrat","role":"aut","given":"Walee"},{"family":"Stremmel","display":"Stremmel, Wolfgang","role":"aut","given":"Wolfgang"},{"family":"Pathil-Warth","display":"Pathil-Warth, Anita","given":"Anita","role":"aut"}]} | ||
| SRT | |a SUJIEGANSCBIVALENTLI2020 | ||