Cross-reactivity of antibodies directed to the gram-negative bacterium Neisseria gonorrhoeae with heat shock protein 60 and ATP-binding protein correlates to reduced mitochondrial activity in HIBCPP choroid plexus papilloma cells
Antibacterial antibodies can cause neurologic side-effects by cross-reactivity with cellular antigens. Here we investigated interactions of antibodies to Neisseria gonorrhoeae (α-NG) - maternal infections by which increases the offspring’s risk for later psychosis—with HIBCPP cells, a cell culture m...
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| Hauptverfasser: | , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
17 June 2015
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| In: |
Journal of molecular neuroscience
Year: 2015, Jahrgang: 57, Heft: 1, Pages: 123-138 |
| ISSN: | 1559-1166 |
| DOI: | 10.1007/s12031-015-0585-7 |
| Online-Zugang: | Verlag, Volltext: http://dx.doi.org/10.1007/s12031-015-0585-7 Verlag, Volltext: https://doi.org/10.1007/s12031-015-0585-7 
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| Verfasserangaben: | B. Reuss, H. Schroten, H. Ishikawa, A.R. Asif |
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| 245 | 1 | 0 | |a Cross-reactivity of antibodies directed to the gram-negative bacterium Neisseria gonorrhoeae with heat shock protein 60 and ATP-binding protein correlates to reduced mitochondrial activity in HIBCPP choroid plexus papilloma cells |c B. Reuss, H. Schroten, H. Ishikawa, A.R. Asif |
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| 520 | |a Antibacterial antibodies can cause neurologic side-effects by cross-reactivity with cellular antigens. Here we investigated interactions of antibodies to Neisseria gonorrhoeae (α-NG) - maternal infections by which increases the offspring’s risk for later psychosis—with HIBCPP cells, a cell culture model of choroid plexus epithelium. Immunocytochemistry and Western blotting with α-NG, revealed organelle-like intracellular staining in HIBCPP cells, and labelling of several immunoreactive bands in cellular protein. Two-dimensional Western blotting revealed several immunopositive spots, most prominent of which were identified by mass spectrometry as mitochondrially localized proteins heat shock protein 60 (Hsp60) and ATP-binding protein β-subunit (ATPB). Similarly α-NG interacted with commercial samples of these proteins as revealed by Western blotting. Three alternative methods (JC-1, Janus green and MTT staining) revealed α-NG to cause in HIBCPP cells a significant decrease in mitochondrial activity, which could be reverted by neuroleptic drugs. Immunoreactivity of α-NG with choroid plexus epithelium in human post mortem samples suggests in vivo relevance of these findings. Finally, distinctly different staining patterns of antibodies against Neisseria meningitidis (α-NM), confirmed antibody specificity. To our knowledge this is the first report that α-NG cross-reactivity with Hsp60 and ATPB impairs mitochondrial activity in choroid plexus epithelial cells, pathogenetic relevance of which needs further clarification. | ||
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