Microscopy-based high-content screening
Image-based screening is used to measure a variety of phenotypes in cells and whole organisms. Combined with perturbations such as RNA interference, small molecules, and mutations, such screens are a powerful method for gaining systematic insights into biological processes. Screens have been applied...
Gespeichert in:
| Hauptverfasser: | , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
December 3, 2015
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| In: |
Cell
Year: 2015, Jahrgang: 163, Heft: 6, Pages: 1314-1325 |
| ISSN: | 1097-4172 |
| DOI: | 10.1016/j.cell.2015.11.007 |
| Online-Zugang: | Verlag, Volltext: http://dx.doi.org/10.1016/j.cell.2015.11.007 Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S0092867415014877 
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| Verfasserangaben: | Michael Boutros, Florian Heigwer, and Christina Laufer |
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| 520 | |a Image-based screening is used to measure a variety of phenotypes in cells and whole organisms. Combined with perturbations such as RNA interference, small molecules, and mutations, such screens are a powerful method for gaining systematic insights into biological processes. Screens have been applied to study diverse processes, such as protein-localization changes, cancer cell vulnerabilities, and complex organismal phenotypes. Recently, advances in imaging and image-analysis methodologies have accelerated large-scale perturbation screens. Here, we describe the state of the art for image-based screening experiments and delineate experimental approaches and image-analysis approaches as well as discussing challenges and future directions, including leveraging CRISPR/Cas9-mediated genome engineering. | ||
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