Buchumschlag

Cdk12 is a gene-selective RNA polymerase II kinase that regulates a subset of the transcriptome, including Nrf2 target genes

The Nrf2 transcription factor is well conserved throughout metazoan evolution and serves as a central regulator of adaptive cellular responses to oxidative stress. We carried out an RNAi screen in Drosophila S2 cells to better understand the regulatory mechanisms governing Nrf2 target gene expressio...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Li, Xuan (VerfasserIn) , Boutros, Michael (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 25 February 2016
In: Scientific reports
Year: 2016, Jahrgang: 6
ISSN:2045-2322
DOI:10.1038/srep21455
Online-Zugang:Verlag, Volltext: http://dx.doi.org/10.1038/srep21455
Verlag, Volltext: https://www-nature-com.ezproxy.medma.uni-heidelberg.de/articles/srep21455
Volltext
Verfasserangaben:Xuan Li, Nirmalya Chatterjee, Kerstin Spirohn, Michael Boutros and Dirk Bohmann

MARC

LEADER 00000caa a2200000 c 4500
001 1587210800
003 DE-627
005 20220815095837.0
007 cr uuu---uuuuu
008 190204s2016 xx |||||o 00| ||eng c
024 7 |a 10.1038/srep21455  |2 doi 
035 |a (DE-627)1587210800 
035 |a (DE-576)517210800 
035 |a (DE-599)BSZ517210800 
035 |a (OCoLC)1341035068 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 33  |2 sdnb 
100 1 |a Li, Xuan  |e VerfasserIn  |0 (DE-588)1176983946  |0 (DE-627)1048761436  |0 (DE-576)517209969  |4 aut 
245 1 0 |a Cdk12 is a gene-selective RNA polymerase II kinase that regulates a subset of the transcriptome, including Nrf2 target genes  |c Xuan Li, Nirmalya Chatterjee, Kerstin Spirohn, Michael Boutros and Dirk Bohmann 
264 1 |c 25 February 2016 
300 |a 13 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Gesehen am 04.02.2019 
520 |a The Nrf2 transcription factor is well conserved throughout metazoan evolution and serves as a central regulator of adaptive cellular responses to oxidative stress. We carried out an RNAi screen in Drosophila S2 cells to better understand the regulatory mechanisms governing Nrf2 target gene expression. This paper describes the identification and characterization of the RNA polymerase II (Pol II) kinase Cdk12 as a factor that is required for Nrf2 target gene expression in cell culture and in vivo. Cdk12 is, however, not essential for bulk mRNA transcription and cells lacking CDK12 function are viable and able to proliferate. Consistent with previous findings on the DNA damage and heat shock responses, it emerges that Cdk12 may be specifically required for stress activated gene expression. Transcriptome analysis revealed that antioxidant gene expression is compromised in flies with reduced Cdk12 function, which makes them oxidative stress sensitive. In addition to supporting Reactive Oxygen Species (ROS) induced gene activation, Cdk12 suppresses genes that support metabolic functions in stressed conditions. We suggest that Cdk12 acts as a gene-selective Pol II kinase that engages a global shift in gene expression to switch cells from a metabolically active state to “stress-defence mode” when challenged by external stress. 
700 1 |a Boutros, Michael  |e VerfasserIn  |0 (DE-588)1023031132  |0 (DE-627)717350045  |0 (DE-576)366291831  |4 aut 
773 0 8 |i Enthalten in  |t Scientific reports  |d [London] : Springer Nature, 2011  |g 6(2016) Artikel-Nummer 21455, 13 Seiten  |h Online-Ressource  |w (DE-627)663366712  |w (DE-600)2615211-3  |w (DE-576)346641179  |x 2045-2322  |7 nnas  |a Cdk12 is a gene-selective RNA polymerase II kinase that regulates a subset of the transcriptome, including Nrf2 target genes 
773 1 8 |g volume:6  |g year:2016  |g extent:13  |a Cdk12 is a gene-selective RNA polymerase II kinase that regulates a subset of the transcriptome, including Nrf2 target genes 
856 4 0 |u http://dx.doi.org/10.1038/srep21455  |x Verlag  |x Resolving-System  |3 Volltext 
856 4 0 |u https://www-nature-com.ezproxy.medma.uni-heidelberg.de/articles/srep21455  |x Verlag  |3 Volltext 
951 |a AR 
992 |a 20190204 
993 |a Article 
994 |a 2016 
998 |g 1023031132  |a Boutros, Michael  |m 1023031132:Boutros, Michael  |d 60000  |e 60000PB1023031132  |k 0/60000/  |p 4 
999 |a KXP-PPN1587210800  |e 3053760665 
BIB |a Y 
SER |a journal 
JSO |a {"title":[{"title_sort":"Cdk12 is a gene-selective RNA polymerase II kinase that regulates a subset of the transcriptome, including Nrf2 target genes","title":"Cdk12 is a gene-selective RNA polymerase II kinase that regulates a subset of the transcriptome, including Nrf2 target genes"}],"language":["eng"],"relHost":[{"type":{"bibl":"periodical","media":"Online-Ressource"},"id":{"zdb":["2615211-3"],"issn":["2045-2322"],"eki":["663366712"]},"pubHistory":["1, article number 1 (2011)-"],"recId":"663366712","origin":[{"publisher":"Springer Nature ; Nature Publishing Group","dateIssuedKey":"2011","dateIssuedDisp":"2011-","publisherPlace":"[London] ; London"}],"physDesc":[{"extent":"Online-Ressource"}],"note":["Gesehen am 12.07.24"],"title":[{"title_sort":"Scientific reports","title":"Scientific reports"}],"language":["eng"],"disp":"Cdk12 is a gene-selective RNA polymerase II kinase that regulates a subset of the transcriptome, including Nrf2 target genesScientific reports","part":{"year":"2016","extent":"13","text":"6(2016) Artikel-Nummer 21455, 13 Seiten","volume":"6"}}],"note":["Gesehen am 04.02.2019"],"recId":"1587210800","physDesc":[{"extent":"13 S."}],"origin":[{"dateIssuedDisp":"25 February 2016","dateIssuedKey":"2016"}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"name":{"displayForm":["Xuan Li, Nirmalya Chatterjee, Kerstin Spirohn, Michael Boutros and Dirk Bohmann"]},"id":{"eki":["1587210800"],"doi":["10.1038/srep21455"]},"person":[{"family":"Li","display":"Li, Xuan","role":"aut","given":"Xuan"},{"role":"aut","given":"Michael","family":"Boutros","display":"Boutros, Michael"}]} 
SRT |a LIXUANBOUTCDK12ISAGE2520 

Ähnliche Einträge