The efficiency of heart protection with HTK or HTK-N depending on the type of ischemia
We investigated isolated guinea pig hearts (n=121) in an ischemia/ reperfusion model with the aim to compare the efficiency of the cardioplegic solution HTK with its novel replacement HTKN. Following consolidation with Tyrode's solution, ischemia started either immediately or after preceding ca...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
2019
|
| In: |
Bioelectrochemistry
Year: 2018, Volume: 125, Pages: 58-69 |
| ISSN: | 1878-562X |
| DOI: | 10.1016/j.bioelechem.2018.09.005 |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1016/j.bioelechem.2018.09.005 Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S1567539418303098 
|
| Author Notes: | Michael Schaefer, Martha-Maria Gebhard, Wolfgang Gross |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1587825783 | ||
| 003 | DE-627 | ||
| 005 | 20220815110118.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 190220r20192018xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.bioelechem.2018.09.005 |2 doi | |
| 035 | |a (DE-627)1587825783 | ||
| 035 | |a (DE-576)517825783 | ||
| 035 | |a (DE-599)BSZ517825783 | ||
| 035 | |a (OCoLC)1341038858 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Schäfer, Michael |d 1962- |e VerfasserIn |0 (DE-588)121913007 |0 (DE-627)705700550 |0 (DE-576)29298877X |4 aut | |
| 245 | 1 | 4 | |a The efficiency of heart protection with HTK or HTK-N depending on the type of ischemia |c Michael Schaefer, Martha-Maria Gebhard, Wolfgang Gross |
| 264 | 1 | |c 2019 | |
| 300 | |a 12 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Available online 20 September 2018 | ||
| 500 | |a Gesehen am 20.02.2019 | ||
| 520 | |a We investigated isolated guinea pig hearts (n=121) in an ischemia/ reperfusion model with the aim to compare the efficiency of the cardioplegic solution HTK with its novel replacement HTKN. Following consolidation with Tyrode's solution, ischemia started either immediately or after preceding cardioplegia with HTK, HTKN, or modified HTK enriched with Ca. Ischemia lasted either 80min at 30°C, or 360min at 5°C, or 81min at 30°C with intermittent cardioplegic perfusion. During ischemia we measured intracellular calcium (iCa++) and the time of gap junction uncoupling (t-in). During reperfusion we measured the reestablishment of cell coupling (t-ret), left ventricular developed pressure (LVDP), and heart rhythm (VC-RR). In 5°C groups, iCa++ at t-in was significantly higher than before ischemia, and longest t-in, shortest t-ret, and best VC-RR were observed after HTK-protection. Of all 30°C groups, the intermittent group with modified HTK showed shortest t-ret, best VC-RR, and the highest LVDP. At 5°C, HTK groups had higher LVDP than HTK-N groups, but not at 30°C. The data suggest that the higher calcium level in the HTK-N solution improves reperfusion after short ischemia at 30°C but for long lasting ischemia at 5°C it is beneficial to use the HTK solution. | ||
| 534 | |c 2018 | ||
| 650 | 4 | |a Calcium | |
| 650 | 4 | |a Cardioplegia | |
| 650 | 4 | |a Gap junctions | |
| 650 | 4 | |a Heart protection | |
| 650 | 4 | |a Ischemia | |
| 650 | 4 | |a Reperfusion | |
| 700 | 1 | |a Gebhard, Martha-Maria |d 1948- |e VerfasserIn |0 (DE-588)1076763278 |0 (DE-627)835312879 |0 (DE-576)445612789 |4 aut | |
| 700 | 1 | |a Gross, Wolfgang |d 1954- |e VerfasserIn |0 (DE-588)1074045599 |0 (DE-627)83021576X |0 (DE-576)435557963 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Bioelectrochemistry |d Amsterdam [u.a.] : Elsevier Science, 2000 |g 125(2019), Seite 58-69 |h Online-Ressource |w (DE-627)306323486 |w (DE-600)1496503-3 |w (DE-576)10419412X |x 1878-562X |7 nnas |a The efficiency of heart protection with HTK or HTK-N depending on the type of ischemia |
| 773 | 1 | 8 | |g volume:125 |g year:2019 |g pages:58-69 |g extent:12 |a The efficiency of heart protection with HTK or HTK-N depending on the type of ischemia |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.bioelechem.2018.09.005 |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u http://www.sciencedirect.com/science/article/pii/S1567539418303098 |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20190220 | ||
| 993 | |a Article | ||
| 994 | |a 2019 | ||
| 998 | |g 1074045599 |a Gross, Wolfgang |m 1074045599:Gross, Wolfgang |d 910000 |d 910200 |e 910000PG1074045599 |e 910200PG1074045599 |k 0/910000/ |k 1/910000/910200/ |p 3 |y j | ||
| 998 | |g 1076763278 |a Gebhard, Martha-Maria |m 1076763278:Gebhard, Martha-Maria |d 50000 |e 50000PG1076763278 |k 0/50000/ |p 2 | ||
| 998 | |g 121913007 |a Schäfer, Michael |m 121913007:Schäfer, Michael |d 910000 |d 910200 |e 910000PS121913007 |e 910200PS121913007 |k 0/910000/ |k 1/910000/910200/ |p 1 |x j | ||
| 999 | |a KXP-PPN1587825783 |e 3056242118 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"note":["Available online 20 September 2018","Gesehen am 20.02.2019"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"recId":"1587825783","language":["eng"],"person":[{"family":"Schäfer","given":"Michael","roleDisplay":"VerfasserIn","display":"Schäfer, Michael","role":"aut"},{"role":"aut","display":"Gebhard, Martha-Maria","roleDisplay":"VerfasserIn","given":"Martha-Maria","family":"Gebhard"},{"given":"Wolfgang","family":"Gross","role":"aut","display":"Gross, Wolfgang","roleDisplay":"VerfasserIn"}],"title":[{"title":"The efficiency of heart protection with HTK or HTK-N depending on the type of ischemia","title_sort":"efficiency of heart protection with HTK or HTK-N depending on the type of ischemia"}],"physDesc":[{"extent":"12 S."}],"relHost":[{"physDesc":[{"extent":"Online-Ressource"}],"id":{"eki":["306323486"],"zdb":["1496503-3"],"issn":["1878-562X"]},"origin":[{"dateIssuedDisp":"2000-","publisher":"Elsevier Science","dateIssuedKey":"2000","publisherPlace":"Amsterdam [u.a.]"}],"part":{"volume":"125","text":"125(2019), Seite 58-69","extent":"12","year":"2019","pages":"58-69"},"pubHistory":["51.2000 - 82.2011; Vol. 83.2012 -"],"corporate":[{"role":"isb","roleDisplay":"Herausgebendes Organ","display":"Bioelectrochemical Society"}],"language":["eng"],"recId":"306323486","type":{"bibl":"periodical","media":"Online-Ressource"},"note":["Gesehen am 09.11.2020"],"disp":"The efficiency of heart protection with HTK or HTK-N depending on the type of ischemiaBioelectrochemistry","title":[{"subtitle":"official journal of the Bioelectrochemical Society ; an international journal devoted to electrochemical aspects of biology and biological aspects of electrochemistry","title":"Bioelectrochemistry","title_sort":"Bioelectrochemistry"}]}],"name":{"displayForm":["Michael Schaefer, Martha-Maria Gebhard, Wolfgang Gross"]},"origin":[{"dateIssuedDisp":"2019","dateIssuedKey":"2019"}],"id":{"eki":["1587825783"],"doi":["10.1016/j.bioelechem.2018.09.005"]}} | ||
| SRT | |a SCHAEFERMIEFFICIENCY2019 | ||