MicroRNA-27a/b controls endothelial cell repulsion and angiogenesis by targeting semaphorin 6A
MicroRNAs (miRs) are small RNAs that regulate gene expression at the posttranscriptional level. miR-27 is expressed in endothelial cells, but the specific functions of miR-27b and its family member miR-27a are largely unknown. Here we demonstrate that overexpression of miR-27a and miR-27b significan...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
2012
|
| In: |
Blood
Year: 2012, Volume: 119, Issue: 6, Pages: 1607-1616 |
| ISSN: | 1528-0020 |
| DOI: | 10.1182/blood-2011-08-373886 |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1182/blood-2011-08-373886 Verlag, Volltext: http://www.bloodjournal.org/content/119/6/1607 
|
| Author Notes: | Carmen Urbich, David Kaluza, Timo Frömel, Andrea Knau, Katrin Bennewitz, Reinier A. Boon, Angelika Bonauer, Carmen Doebele, Jes-Niels Boeckel, Eduard Hergenreider, Andreas M. Zeiher, Jens Kroll, Ingrid Fleming, Stefanie Dimmeler |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 158787251X | ||
| 003 | DE-627 | ||
| 005 | 20220815110936.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 190221s2012 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1182/blood-2011-08-373886 |2 doi | |
| 035 | |a (DE-627)158787251X | ||
| 035 | |a (DE-576)51787251X | ||
| 035 | |a (DE-599)BSZ51787251X | ||
| 035 | |a (OCoLC)1341039420 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Urbich, Carmen |d 1973- |e VerfasserIn |0 (DE-588)123013593 |0 (DE-627)08229447X |0 (DE-576)187082227 |4 aut | |
| 245 | 1 | 0 | |a MicroRNA-27a/b controls endothelial cell repulsion and angiogenesis by targeting semaphorin 6A |c Carmen Urbich, David Kaluza, Timo Frömel, Andrea Knau, Katrin Bennewitz, Reinier A. Boon, Angelika Bonauer, Carmen Doebele, Jes-Niels Boeckel, Eduard Hergenreider, Andreas M. Zeiher, Jens Kroll, Ingrid Fleming, Stefanie Dimmeler |
| 264 | 1 | |c 2012 | |
| 300 | |a 10 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Prepublished online as Blood first edition paper, December 19, 2011 | ||
| 500 | |a Gesehen am 21.02.2019 | ||
| 520 | |a MicroRNAs (miRs) are small RNAs that regulate gene expression at the posttranscriptional level. miR-27 is expressed in endothelial cells, but the specific functions of miR-27b and its family member miR-27a are largely unknown. Here we demonstrate that overexpression of miR-27a and miR-27b significantly increased endothelial cell sprouting. Inhibition of both miR-27a and miR-27b impaired endothelial cell sprout formation and induced endothelial cell repulsion in vitro. In vivo, inhibition of miR-27a/b decreased the number of perfused vessels in Matrigel plugs and impaired embryonic vessel formation in zebrafish. Mechanistically, miR-27 regulated the expression of the angiogenesis inhibitor semaphorin 6A (SEMA6A) in vitro and in vivo and targeted the 3′-untranslated region of SEMA6A. Silencing of SEMA6A partially reversed the inhibition of endothelial cell sprouting and abrogated the repulsion of endothelial cells mediated by miR-27a/b inhibition, indicating that SEMA6A is a functionally relevant miR-27 downstream target regulating endothelial cell repulsion. In summary, we show that miR-27a/b promotes angiogenesis by targeting the angiogenesis inhibitor SEMA6A, which controls repulsion of neighboring endothelial cells. | ||
| 700 | 1 | |a Bennewitz, Katrin |d 1981- |e VerfasserIn |0 (DE-588)1154553140 |0 (DE-627)1015942172 |0 (DE-576)501101209 |4 aut | |
| 700 | 1 | |a Kroll, Jens |d 1969- |e VerfasserIn |0 (DE-588)1032295619 |0 (DE-627)738209503 |0 (DE-576)379947544 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Blood |d Washington, DC : American Society of Hematology, 1946 |g 119(2012), 6, Seite 1607-1616 |h Online-Ressource |w (DE-627)266886647 |w (DE-600)1468538-3 |w (DE-576)075961938 |x 1528-0020 |7 nnas |a MicroRNA-27a/b controls endothelial cell repulsion and angiogenesis by targeting semaphorin 6A |
| 773 | 1 | 8 | |g volume:119 |g year:2012 |g number:6 |g pages:1607-1616 |g extent:10 |a MicroRNA-27a/b controls endothelial cell repulsion and angiogenesis by targeting semaphorin 6A |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1182/blood-2011-08-373886 |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u http://www.bloodjournal.org/content/119/6/1607 |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20190221 | ||
| 993 | |a Article | ||
| 994 | |a 2012 | ||
| 998 | |g 1032295619 |a Kroll, Jens |m 1032295619:Kroll, Jens |d 60000 |d 999810 |e 60000PK1032295619 |e 999810PK1032295619 |k 0/60000/ |k 1/60000/999810/ |p 12 | ||
| 998 | |g 1154553140 |a Bennewitz, Katrin |m 1154553140:Bennewitz, Katrin |d 60000 |d 999810 |e 60000PB1154553140 |e 999810PB1154553140 |k 0/60000/ |k 1/60000/999810/ |p 5 | ||
| 999 | |a KXP-PPN158787251X |e 305632274X | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"id":{"eki":["158787251X"],"doi":["10.1182/blood-2011-08-373886"]},"note":["Prepublished online as Blood first edition paper, December 19, 2011","Gesehen am 21.02.2019"],"recId":"158787251X","language":["eng"],"name":{"displayForm":["Carmen Urbich, David Kaluza, Timo Frömel, Andrea Knau, Katrin Bennewitz, Reinier A. Boon, Angelika Bonauer, Carmen Doebele, Jes-Niels Boeckel, Eduard Hergenreider, Andreas M. Zeiher, Jens Kroll, Ingrid Fleming, Stefanie Dimmeler"]},"relHost":[{"id":{"zdb":["1468538-3"],"eki":["266886647"],"issn":["1528-0020"]},"note":["Gesehen am 21.04.2023"],"recId":"266886647","type":{"bibl":"periodical","media":"Online-Ressource"},"disp":"MicroRNA-27a/b controls endothelial cell repulsion and angiogenesis by targeting semaphorin 6ABlood","physDesc":[{"extent":"Online-Ressource"}],"part":{"extent":"10","year":"2012","volume":"119","pages":"1607-1616","text":"119(2012), 6, Seite 1607-1616","issue":"6"},"language":["eng"],"origin":[{"dateIssuedKey":"1946","publisherPlace":"Washington, DC ; Philadelphia, Pa. ; Stanford, Calif.","publisher":"American Society of Hematology ; Saunders ; HighWire Press","dateIssuedDisp":"1946-"}],"corporate":[{"role":"isb","roleDisplay":"Herausgebendes Organ","display":"American Society of Hematology"}],"pubHistory":["1.1946 -"],"titleAlt":[{"title":"Blood online"}],"title":[{"title_sort":"Blood","title":"Blood","subtitle":"journal of the American Society of Hematology"}]}],"origin":[{"dateIssuedDisp":"2012","dateIssuedKey":"2012"}],"type":{"media":"Online-Ressource","bibl":"article-journal"},"physDesc":[{"extent":"10 S."}],"person":[{"role":"aut","roleDisplay":"VerfasserIn","given":"Carmen","display":"Urbich, Carmen","family":"Urbich"},{"roleDisplay":"VerfasserIn","given":"Katrin","role":"aut","family":"Bennewitz","display":"Bennewitz, Katrin"},{"family":"Kroll","display":"Kroll, Jens","given":"Jens","roleDisplay":"VerfasserIn","role":"aut"}],"title":[{"title":"MicroRNA-27a/b controls endothelial cell repulsion and angiogenesis by targeting semaphorin 6A","title_sort":"MicroRNA-27a/b controls endothelial cell repulsion and angiogenesis by targeting semaphorin 6A"}]} | ||
| SRT | |a URBICHCARMMICRORNA272012 | ||