Buchumschlag

Attenuation of renovascular damage in zucker diabetic fatty rat by NWT-03, an egg protein hydrolysate with ACE- and DPP4-Inhibitory activity

Background Dipeptidyl peptidase 4 (DPP4) and angiotensin-converting enzyme (ACE) are important target enzymes in glycemic control and renovascular protection. Here, we studied the effect of NWT-03, an egg protein hydrolysate with DPP4- and ACE-inhibitory activity, on renovascular damage in Zucker di...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Wang, Yumei (VerfasserIn) , Landheer, Sjoerd (VerfasserIn) , Hammes, Hans-Peter (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: October 10, 2012
In: PLOS ONE
Year: 2012, Jahrgang: 7, Heft: 10
ISSN:1932-6203
DOI:10.1371/journal.pone.0046781
Online-Zugang:Verlag, Volltext: http://dx.doi.org/10.1371/journal.pone.0046781
Verlag, Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0046781
Volltext
Verfasserangaben:Yumei Wang, Sjoerd Landheer, Wiek H. van Gilst, Aart van Amerongen, Hans-Peter Hammes, Robert H. Henning, Leo E. Deelman, Hendrik Buikema

MARC

LEADER 00000caa a2200000 c 4500
001 1588047792
003 DE-627
005 20220815112135.0
007 cr uuu---uuuuu
008 190226s2012 xx |||||o 00| ||eng c
024 7 |a 10.1371/journal.pone.0046781  |2 doi 
035 |a (DE-627)1588047792 
035 |a (DE-576)518047792 
035 |a (DE-599)BSZ518047792 
035 |a (OCoLC)1341040020 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 33  |2 sdnb 
100 1 |a Wang, Yumei  |d 1974-  |e VerfasserIn  |0 (DE-588)1019459492  |0 (DE-627)690907605  |0 (DE-576)356088383  |4 aut 
245 1 0 |a Attenuation of renovascular damage in zucker diabetic fatty rat by NWT-03, an egg protein hydrolysate with ACE- and DPP4-Inhibitory activity  |c Yumei Wang, Sjoerd Landheer, Wiek H. van Gilst, Aart van Amerongen, Hans-Peter Hammes, Robert H. Henning, Leo E. Deelman, Hendrik Buikema 
264 1 |c October 10, 2012 
300 |a 11 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Gesehen am 26.02.2019 
520 |a Background Dipeptidyl peptidase 4 (DPP4) and angiotensin-converting enzyme (ACE) are important target enzymes in glycemic control and renovascular protection. Here, we studied the effect of NWT-03, an egg protein hydrolysate with DPP4- and ACE-inhibitory activity, on renovascular damage in Zucker diabetic fatty (ZDF) rats. Comparisons were made to rats treated with vildagliptin (VIL), included as a positive control for the effect of DPP4 inhibition. Methods ZDF rats received NWT-03 (1 g/kg/day) or VIL (3 mg/kg/day) from 10 to 25 weeks of age. Metabolic and renal functions were assessed; the kidney was removed for histological analysis of glomerulosclerosis and expression of pro-inflammatory/fibrotic markers (RT-PCR and Western blotting); and the aorta was removed for studies of endothelium-dependent relaxation (EDR). Findings Hyperinsulinemic ZDF rats typically developed signs of type-2 diabetes and renovascular damage, as evidenced by albuminuria, glomerulosclerosis, and impaired EDR. Neither NWT-03 nor VIL improved metabolic parameters; for VIL, this was despite a 5-fold increase in glucagon-like peptide (GLP)-1 levels. NWT-03 and VIL both reduced renal interleukin (Il)-1β/Il-13 mRNA expression and glomerulosclerosis. However, only NWT-03 additionally decreased renal tumor necrosis factor (TNF)-α mRNA and P22phox protein expression, reduced albuminuria, and restored aortic EDR. Indomethacin added to the organ bath instantly improved aortic EDR, indicating a role for cyclooxygenase (COX)-derived contractile prostanoids in opposing relaxation in ZDF rats. This indomethacin effect was reduced by NWT-03, but not by VIL, and coincided with decreased renal COX-1/2 protein expression. Conclusion and Interpretation Long-term supplementation with the egg protein hydrolysate NWT-03 attenuated renovascular damage in this preclinical rat model of type 2 diabetes. A comparison to the DPP4-inhibitor VIL suggests that the effects of NWT-03 were related to both ACE- and DPP4-inhibitory properties. The development of protein hydrolysates with a multiple-targeting strategy may be of benefit to functional food formulations. 
650 4 |a Aorta 
650 4 |a Blood plasma 
650 4 |a Blood pressure 
650 4 |a Diabetes mellitus 
650 4 |a Glucose 
650 4 |a Glucose metabolism 
650 4 |a Kidneys 
650 4 |a Protein expression 
700 1 |a Landheer, Sjoerd  |e VerfasserIn  |0 (DE-588)117920106X  |0 (DE-627)106659564X  |0 (DE-576)518047636  |4 aut 
700 1 |a Hammes, Hans-Peter  |d 1955-  |e VerfasserIn  |0 (DE-588)102028353X  |0 (DE-627)691169772  |0 (DE-576)360007252  |4 aut 
773 0 8 |i Enthalten in  |t PLOS ONE  |d San Francisco, California, US : PLOS, 2006  |g 7(2012,10) Artikel-Nummer e46781, 11Seiten  |h Online-Ressource  |w (DE-627)523574592  |w (DE-600)2267670-3  |w (DE-576)281331979  |x 1932-6203  |7 nnas  |a Attenuation of renovascular damage in zucker diabetic fatty rat by NWT-03, an egg protein hydrolysate with ACE- and DPP4-Inhibitory activity 
773 1 8 |g volume:7  |g year:2012  |g number:10  |g extent:11  |a Attenuation of renovascular damage in zucker diabetic fatty rat by NWT-03, an egg protein hydrolysate with ACE- and DPP4-Inhibitory activity 
856 4 0 |u http://dx.doi.org/10.1371/journal.pone.0046781  |x Verlag  |x Resolving-System  |3 Volltext 
856 4 0 |u https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0046781  |x Verlag  |3 Volltext 
951 |a AR 
992 |a 20190226 
993 |a Article 
994 |a 2012 
998 |g 102028353X  |a Hammes, Hans-Peter  |m 102028353X:Hammes, Hans-Peter  |d 60000  |d 61400  |e 60000PH102028353X  |e 61400PH102028353X  |k 0/60000/  |k 1/60000/61400/  |p 5 
998 |g 1019459492  |a Wang, Yumei  |m 1019459492:Wang, Yumei  |d 60000  |d 61400  |e 60000PW1019459492  |e 61400PW1019459492  |k 0/60000/  |k 1/60000/61400/  |p 1  |x j 
999 |a KXP-PPN1588047792  |e 3056669596 
BIB |a Y 
SER |a journal 
JSO |a {"physDesc":[{"extent":"11 S."}],"id":{"doi":["10.1371/journal.pone.0046781"],"eki":["1588047792"]},"type":{"media":"Online-Ressource","bibl":"article-journal"},"title":[{"title":"Attenuation of renovascular damage in zucker diabetic fatty rat by NWT-03, an egg protein hydrolysate with ACE- and DPP4-Inhibitory activity","title_sort":"Attenuation of renovascular damage in zucker diabetic fatty rat by NWT-03, an egg protein hydrolysate with ACE- and DPP4-Inhibitory activity"}],"origin":[{"dateIssuedDisp":"October 10, 2012","dateIssuedKey":"2012"}],"relHost":[{"id":{"eki":["523574592"],"issn":["1932-6203"],"zdb":["2267670-3"]},"physDesc":[{"extent":"Online-Ressource"}],"title":[{"title":"PLOS ONE","title_sort":"PLOS ONE"}],"origin":[{"dateIssuedKey":"2006","publisherPlace":"San Francisco, California, US ; Lawrence, Kan.","publisher":"PLOS ; PLoS","dateIssuedDisp":"2006-"}],"name":{"displayForm":["Public Library of Science"]},"recId":"523574592","disp":"Attenuation of renovascular damage in zucker diabetic fatty rat by NWT-03, an egg protein hydrolysate with ACE- and DPP4-Inhibitory activityPLOS ONE","type":{"media":"Online-Ressource","bibl":"periodical"},"corporate":[{"role":"isb","display":"Public Library of Science"}],"part":{"text":"7(2012,10) Artikel-Nummer e46781, 11Seiten","extent":"11","issue":"10","year":"2012","volume":"7"},"language":["eng"],"note":["Schreibweise des Titels bis 2012: PLoS ONE","Gesehen am 20.03.19"],"pubHistory":["1.2006 -"]}],"language":["eng"],"name":{"displayForm":["Yumei Wang, Sjoerd Landheer, Wiek H. van Gilst, Aart van Amerongen, Hans-Peter Hammes, Robert H. Henning, Leo E. Deelman, Hendrik Buikema"]},"note":["Gesehen am 26.02.2019"],"recId":"1588047792","person":[{"role":"aut","family":"Wang","given":"Yumei","display":"Wang, Yumei"},{"given":"Sjoerd","display":"Landheer, Sjoerd","role":"aut","family":"Landheer"},{"display":"Hammes, Hans-Peter","given":"Hans-Peter","family":"Hammes","role":"aut"}]} 
SRT |a WANGYUMEILATTENUATIO1020 

Ähnliche Einträge