Identification of novel proteins associated with the development of chemoresistance in malignant melanoma using two-dimensional electrophoresis
A model system for studying chemoresistance in human melanoma cells (MeWo) has been established utilizing the four commonly used cytotoxic drugs vindesine, cisplatin, fotemustine and etoposide to yield stable drug-resistant sublines. We analyzed phenotypical differences between MeWo cells and their...
Gespeichert in:
| Hauptverfasser: | , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
24 August 2000
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| In: |
Electrophoresis
Year: 2000, Jahrgang: 21, Heft: 14, Pages: 3048-3057 |
| ISSN: | 1522-2683 |
| DOI: | 10.1002/1522-2683(20000801)21:14<3048::AID-ELPS3048>3.0.CO;2-W |
| Online-Zugang: | Verlag, Volltext: http://dx.doi.org/10.1002/1522-2683(20000801)21:14<3048::AID-ELPS3048>3.0.CO;2-W Verlag, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/1522-2683%2820000801%2921%3A14%3C3048%3A%3AAID-ELPS3048%3E3.0.CO%3B2-W 
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| Verfasserangaben: | Pranav Sinha, Sandra Kohl, Jochen Fischer, Gero Hütter, Monika Kern, Eckard Köttgen, Manfred Dietel, Hermann Lage, Martina Schnölzer, Dirk Schadendorf |
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| 245 | 1 | 0 | |a Identification of novel proteins associated with the development of chemoresistance in malignant melanoma using two-dimensional electrophoresis |c Pranav Sinha, Sandra Kohl, Jochen Fischer, Gero Hütter, Monika Kern, Eckard Köttgen, Manfred Dietel, Hermann Lage, Martina Schnölzer, Dirk Schadendorf |
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| 520 | |a A model system for studying chemoresistance in human melanoma cells (MeWo) has been established utilizing the four commonly used cytotoxic drugs vindesine, cisplatin, fotemustine and etoposide to yield stable drug-resistant sublines. We analyzed phenotypical differences between MeWo cells and their chemoresistant counterparts using two-dimensional electrophoresis. Proteins that were overexpressed in chemoresistant cell lines were purified and identified using matrix assisted laser desorption/ionization-time of flight — mass spectrometry (MALDI-TOF-MS) and microsequencing. Here we show that four proteins, namely the translationally controlled tumor protein, the human elongation factor 1-δ, tetratricopeptide repeat protein and the isoform 14-3-3-γ of the 14-3-3-family are overexpressed in chemoresistant melanoma cell lines. The significance of these findings is now being verified using transfection experiments with the aim of developing more effective chemotherapy protocols. | ||
| 650 | 4 | |a Chemoresistance | |
| 650 | 4 | |a Melanoma cells | |
| 650 | 4 | |a Two-dimensional electrophoresis | |
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