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Identification of novel proteins associated with the development of chemoresistance in malignant melanoma using two-dimensional electrophoresis

A model system for studying chemoresistance in human melanoma cells (MeWo) has been established utilizing the four commonly used cytotoxic drugs vindesine, cisplatin, fotemustine and etoposide to yield stable drug-resistant sublines. We analyzed phenotypical differences between MeWo cells and their...

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Bibliographische Detailangaben
Hauptverfasser: Sinha-von Jaschke, Pranav (VerfasserIn) , Kern, Monika (VerfasserIn) , Schnölzer, Martina (VerfasserIn) , Schadendorf, Dirk (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 24 August 2000
In: Electrophoresis
Year: 2000, Jahrgang: 21, Heft: 14, Pages: 3048-3057
ISSN:1522-2683
DOI:10.1002/1522-2683(20000801)21:14<3048::AID-ELPS3048>3.0.CO;2-W
Online-Zugang:Verlag, Volltext: http://dx.doi.org/10.1002/1522-2683(20000801)21:14<3048::AID-ELPS3048>3.0.CO;2-W
Verlag, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/1522-2683%2820000801%2921%3A14%3C3048%3A%3AAID-ELPS3048%3E3.0.CO%3B2-W
Volltext
Verfasserangaben:Pranav Sinha, Sandra Kohl, Jochen Fischer, Gero Hütter, Monika Kern, Eckard Köttgen, Manfred Dietel, Hermann Lage, Martina Schnölzer, Dirk Schadendorf
Beschreibung
Zusammenfassung:A model system for studying chemoresistance in human melanoma cells (MeWo) has been established utilizing the four commonly used cytotoxic drugs vindesine, cisplatin, fotemustine and etoposide to yield stable drug-resistant sublines. We analyzed phenotypical differences between MeWo cells and their chemoresistant counterparts using two-dimensional electrophoresis. Proteins that were overexpressed in chemoresistant cell lines were purified and identified using matrix assisted laser desorption/ionization-time of flight — mass spectrometry (MALDI-TOF-MS) and microsequencing. Here we show that four proteins, namely the translationally controlled tumor protein, the human elongation factor 1-δ, tetratricopeptide repeat protein and the isoform 14-3-3-γ of the 14-3-3-family are overexpressed in chemoresistant melanoma cell lines. The significance of these findings is now being verified using transfection experiments with the aim of developing more effective chemotherapy protocols.
Beschreibung:Gesehen am 26.02.2019
Beschreibung:Online Resource
ISSN:1522-2683
DOI:10.1002/1522-2683(20000801)21:14<3048::AID-ELPS3048>3.0.CO;2-W

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