Buchumschlag

In vitro cell migration quantification method for scratch assays

The scratch assay is an in vitro technique used to assess the contribution of molecular and cellular mechanisms to cell migration. The assay can also be used to evaluate therapeutic compounds before clinical use. Current quantification methods of scratch assays deal poorly with irregular cell-free a...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Ponce Bobadilla, Ana Victoria (VerfasserIn) , Carraro, Thomas (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 13 February 2019
In: Interface
Year: 2019, Jahrgang: 16, Heft: 151
ISSN:1742-5662
DOI:10.1098/rsif.2018.0709
Online-Zugang:Resolving-System, Volltext: http://dx.doi.org/10.1098/rsif.2018.0709
Verlag, Volltext: https://royalsocietypublishing.org/doi/full/10.1098/rsif.2018.0709
Verlag, Volltext: https://arxiv.org/abs/1806.09219
Volltext
Verfasserangaben:Bobadilla Ana Victoria Ponce, Arévalo Jazmine, Sarró Eduard, Byrne Helen M., Maini Philip K., Carraro Thomas, Balocco Simone, Meseguer Anna, Alarcón Tomás

MARC

LEADER 00000caa a22000002c 4500
001 1588493245
003 DE-627
005 20220815121016.0
007 cr uuu---uuuuu
008 190308s2019 xx |||||o 00| ||eng c
024 7 |a 10.1098/rsif.2018.0709  |2 doi 
035 |a (DE-627)1588493245 
035 |a (DE-576)518493245 
035 |a (DE-599)BSZ518493245 
035 |a (OCoLC)1341041107 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 27  |2 sdnb 
100 1 |a Ponce Bobadilla, Ana Victoria  |d 1989-  |e VerfasserIn  |0 (DE-588)1180084985  |0 (DE-627)1067635092  |0 (DE-576)518493504  |4 aut 
245 1 0 |a In vitro cell migration quantification method for scratch assays  |c Bobadilla Ana Victoria Ponce, Arévalo Jazmine, Sarró Eduard, Byrne Helen M., Maini Philip K., Carraro Thomas, Balocco Simone, Meseguer Anna, Alarcón Tomás 
264 1 |c 13 February 2019 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Gesehen am 08.03.2019 
520 |a The scratch assay is an in vitro technique used to assess the contribution of molecular and cellular mechanisms to cell migration. The assay can also be used to evaluate therapeutic compounds before clinical use. Current quantification methods of scratch assays deal poorly with irregular cell-free areas and crooked leading edges which are features typically present in the experimental data. We introduce a new migration quantification method, called ‘monolayer edge velocimetry’, that permits analysis of low-quality experimental data and better statistical classification of migration rates than standard quantification methods. The new method relies on quantifying the horizontal component of the cell monolayer velocity across the leading edge. By performing a classification test on in silico data, we show that the method exhibits significantly lower statistical errors than standard methods. When applied to in vitro data, our method outperforms standard methods by detecting differences in the migration rates between different cell groups that the other methods could not detect. Application of this new method will enable quantification of migration rates from in vitro scratch assay data that cannot be analysed using existing methods. 
700 1 |a Carraro, Thomas  |e VerfasserIn  |0 (DE-588)13134868X  |0 (DE-627)507963369  |0 (DE-576)25094829X  |4 aut 
773 0 8 |i Enthalten in  |t Interface  |d London : The Royal Society, 2004  |g 16(2019,151) Artikel-Nummer 20180709, 18 Seiten  |h Online-Ressource  |w (DE-627)391783718  |w (DE-600)2156283-0  |w (DE-576)113423152  |x 1742-5662  |7 nnas  |a In vitro cell migration quantification method for scratch assays 
773 1 8 |g volume:16  |g year:2019  |g number:151  |a In vitro cell migration quantification method for scratch assays 
856 4 0 |u http://dx.doi.org/10.1098/rsif.2018.0709  |x Resolving-System  |x Verlag  |3 Volltext 
856 4 0 |u https://royalsocietypublishing.org/doi/full/10.1098/rsif.2018.0709  |x Verlag  |3 Volltext 
856 4 0 |u https://arxiv.org/abs/1806.09219  |x Verlag  |3 Volltext 
951 |a AR 
992 |a 20190308 
993 |a Article 
994 |a 2019 
998 |g 13134868X  |a Carraro, Thomas  |m 13134868X:Carraro, Thomas  |d 700000  |d 708000  |d 110000  |d 110200  |d 110000  |d 110400  |e 700000PC13134868X  |e 708000PC13134868X  |e 110000PC13134868X  |e 110200PC13134868X  |e 110000PC13134868X  |e 110400PC13134868X  |k 0/700000/  |k 1/700000/708000/  |k 0/110000/  |k 1/110000/110200/  |k 0/110000/  |k 1/110000/110400/  |p 6 
998 |g 1180084985  |a Ponce Bobadilla, Ana Victoria  |m 1180084985:Ponce Bobadilla, Ana Victoria  |d 110000  |d 110200  |d 110000  |d 110400  |e 110000PP1180084985  |e 110200PP1180084985  |e 110000PP1180084985  |e 110400PP1180084985  |k 0/110000/  |k 1/110000/110200/  |k 0/110000/  |k 1/110000/110400/  |p 1  |x j 
999 |a KXP-PPN1588493245  |e 3057870279 
BIB |a Y 
SER |a journal 
JSO |a {"type":{"bibl":"article-journal","media":"Online-Ressource"},"note":["Gesehen am 08.03.2019"],"language":["eng"],"relHost":[{"title":[{"subtitle":"journal of the Royal Society ; physical and life sciences","title":"Interface","title_sort":"Interface"}],"pubHistory":["1.2004 -"],"titleAlt":[{"title":"Journal of the Royal Society interface"}],"part":{"year":"2019","issue":"151","text":"16(2019,151) Artikel-Nummer 20180709, 18 Seiten","volume":"16"},"disp":"In vitro cell migration quantification method for scratch assaysInterface","type":{"media":"Online-Ressource","bibl":"periodical"},"note":["Gesehen am 22.06.2020","Fortsetzung der Druck-Ausgabe"],"recId":"391783718","corporate":[{"display":"Royal Society","roleDisplay":"Herausgebendes Organ","role":"isb"}],"language":["eng"],"origin":[{"publisherPlace":"London","dateIssuedDisp":"2004-","publisher":"The Royal Society","dateIssuedKey":"2004"}],"id":{"eki":["391783718"],"zdb":["2156283-0"],"issn":["1742-5662"]},"physDesc":[{"extent":"Online-Ressource"}]}],"recId":"1588493245","person":[{"role":"aut","roleDisplay":"VerfasserIn","display":"Ponce Bobadilla, Ana Victoria","given":"Ana Victoria","family":"Ponce Bobadilla"},{"display":"Carraro, Thomas","roleDisplay":"VerfasserIn","role":"aut","family":"Carraro","given":"Thomas"}],"name":{"displayForm":["Bobadilla Ana Victoria Ponce, Arévalo Jazmine, Sarró Eduard, Byrne Helen M., Maini Philip K., Carraro Thomas, Balocco Simone, Meseguer Anna, Alarcón Tomás"]},"origin":[{"dateIssuedDisp":"13 February 2019","dateIssuedKey":"2019"}],"title":[{"title_sort":"In vitro cell migration quantification method for scratch assays","title":"In vitro cell migration quantification method for scratch assays"}],"id":{"doi":["10.1098/rsif.2018.0709"],"eki":["1588493245"]}} 
SRT |a PONCEBOBADINVITROCEL1320 

Ähnliche Einträge