Functional glycomics
In this 3 volume collection focusing on glycomics, readers will appreciate how such discoveries were made and how such methods can be applied for readers' own research efforts Each chapter has been designed so that enough scientific background will be given in each chapter for further developme...
Gespeichert in:
| Weitere Verfasser: | |
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| Dokumenttyp: | Edited Volume |
| Sprache: | Englisch |
| Veröffentlicht: |
Amsterdam [u.a.]
Elsevier, Acad. Press
2010
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| Ausgabe: | Online-Ausg. |
| Schriftenreihe: | Methods in enzymology
479 |
| In: |
Methods in enzymology (479)
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| Volumes / Articles: | Show Volumes / Articles. |
| Schlagworte: | |
| Online-Zugang: | Verlag, Volltext: https://www.sciencedirect.com/science/bookseries/00766879/479 Verlag, Volltext: http://www.sciencedirect.com/science/bookseries/00766879/479 Verlag, ScienceDirect, Volltext: http://www.sciencedirect.com/science/book/9780123809971 Einführung/Vorwort: https://swbplus.bsz-bw.de/bsz314900136vor.htm Cover: https://swbplus.bsz-bw.de/bsz314900136cov.jpg Kapitel 1: https://swbplus.bsz-bw.de/bsz314900136kap.htm Inhaltsverzeichnis: https://swbplus.bsz-bw.de/bsz314900136inh.htm Aggregator: http://ebooks.ciando.com/book/index.cfm/bok_id/257980 
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| Verfasserangaben: | ed. by Minoru Fukuda |
Inhaltsangabe:
- Front Cover; Methods in Enzymology: Functional Glycomics; Copyright Page; Contents; Contributors; Preface; Methods in Enzymology; Section one: Stem Cell; Chapter One: beta1,4-Galactosyltransferase V: A Growth Regulator in Glioma; 1. Overview; 2. Experimental; 3. Results; 4. Conclusion and Future Direction; Acknowledgments; References; Chapter Two: Roles of Polysialic Acid in Migration and Differentiation of Neural Stem Cells; 1. Overview; Acknowledgments; References; Chapter Three: Structural and Functional Analysis of Chondroitin Sulfate Proteoglycans in the Neural Stem Cell Niche
- 1. Overview2. Immunohistochemistry of Embryonic and Postnatal Mouse Brain Sections; 3. Method to Stain Embryonic Sections for NSPC Markers; 4. Analysis of the Adult Neurogenic Niche and SVZ-Derived Cells by Immunocytochemistry; 5. Immunocytochemistry of Acutely Dissociated Cells; 6. Isolation of NSPCs by Immunopanning or by Immunoisolation Using Paramagnetic Beads (EasySep); 7. Neurosphere Cultures and Various Methods for Their Analysis; 8. Disaccharide Analysis of CS/DS Chains from Embryonic Brain and Conditioned Neurosphere Culture Media and Effect of Sodium C
- 9. Analysis of NSPC-Proliferation In Vitro and In Vivo10. Analysis of CSPG Functions in NSPCs Using Chondroitinase ABC Treatment in Culture; 11. Analysis of Chondroitin Sulfate Functions in the Neural Stem Cell Niche; 12. RT-PCR and Semiquantitative Analysis of the Synthetic Machinery for Glycosaminoglycans; 13. In Situ Hybridization of Sulfotransferases in Tissue and Neurosphere Sections; 14. Microscopy; 15. Conclusion and Outlook; Acknowledgments; References; Chapter Four: Transcript Analysis of Stem Cells; 1. Introduction
- 2. qRT-PCR as a Tool for Determining Transcript Analysis for Glycan-Related Gene Expression3. Examples of the Applications of qRT-PCR Transcript Analysis to Investigate Changes in Glycan-Related Gene Expression in Ste; Acknowledgments; References; Chapter Five: Directing Stem Cell Trafficking via GPS; 1. Overview; 2. Rationale for GPS; 3. Guiding Principles and Method for GPS; 4. Method of a(1,3)-Fucosylation of Cell Surface Using FTVI; 5. Detection of E-Selectin Ligand Expression Following GPS
- 6. Testing for E-Selectin Ligand Activity Using E-Selectin-Ig Chimera (Three-Step Method for Flow Cytometry)7. Summary; Acknowledgments; References; Chapter Six: Functional Assays for the Molecular Chaperone Cosmc; 1. Overview; 2. T-Synthase and Cosmc; 3. T-Synthase Activity Assay; 4. Assay Activity for Function of Cosmc; 5. Conclusion and Future Directions; Acknowledgments; References; Chapter Seven: Core 3-Derived O-Glycans Are Essential for Intestinal Mucus Barrier Function; 1. Introduction; 2. Generation of C3GnT-/- Mice
- 3. Disruption of the C3GnT Gene Eliminates Core 3-Derived O-Glycans and Exposes the Tn Antigen in Murine Colon
- [beta]1,4-galactosyltransferase V: a growth regulator in glioma Jianhai Jiang and Jianxin GuRoles of polysialic acid in migration and differentiation of neural stem cells / Kiyohiko Angata and Minoru Fukuda
- Structural and functional analysis of chondroitin sulfate proteoglycans in the neural stem cell niche Swetlana Sirko ... [et al.]
- Transcript analysis of stem cells Alison V. Nairn, Mitche dela Rosa and Kelley W. Moremen
- Directing stem cell trafficking via GPS Robert Sackstein
- Functional assays for the molecular chaperone cosmc Tongzhong Ju and Richard D. Cummings
- Core 3-derived O-glycans are essential for intestinal mucus barrier function Lijun Xia
- Core3 glycan as tumor suppressor Seung Ho Lee and Minoru Fukuda
- Characterization of mice with targeted deletion of the gene encoding core 2 [beta]1,6-N-acetylglucosaminyltransferase-2 Erica L. Stone ... [et al.]
- Analyzing physiological function of polypeptide GalNAcT-1-deficient mice in humoral immunity Mari Tenno
- [beta]3GnT2 (B3GNT2), a major polylactosamine synthase: analysis of B3gnt2-deficient mice Akira Togayachi ... [er al.]
- Targeted genetic inactivation of N-acetylglucosaminyltransferase-IVa impairs insulin secretion from pancreatic [beta] cells and evokes type 2 diabetes Kazuaki Ohtsuboa
- The Ashwell-Morell receptor Prabhjit K. Grewal
- Roles of GlcNAc-6-O-sulfotransferases in lymphoid and nonlymphoid tissues Hiroto Kawashima
- Core O-glycans required for lymphocyte homing: gene knockout mice of core 1 [beta]1,3-N-acetylglucosaminyltransferase and core 2 N-acetylglucosaminyltransferase Junya Mitoma and Minoru Fukuda
- Immunohistochemical analysis of carbohydrate antigens in chronic inflammatory gastrointestinal diseases Motohiro Kobayashi and Jun Nakayama
- Genetic defects in muscular dystrophy Kumaran Chandrasekharan and Paul T. Martin
- POMT1 is essential for protein O-mannosylation in mammals Mark Lommel ... [et al.]
- POMGnT1, POMT1, and POMT2 mutations in congenital muscular dystrophies Tamao Endo ... [et al.]
- Cellular and molecular characterization of abnormal brain development in protein O-mannose N-acetylglucosaminyltransferase 1 knockout mice Jianmin Liu ... [et al.]
- Investigating the functions of LARGE: lessons from mutant mice Jane E. Hewitt
- A tumor suppressor function of laminin-binding [alpha]-dystroglycan Xingfeng Bao and Minoru Fukuda
- Tumor formation assays Shingo Hatakeyama, Hayato Yamamoto and Chikara Ohyama.