Calcium signaling
Md. Shahidul Islam
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Dokumenttyp: | Buch/Monographie |
| Sprache: | Englisch |
| Veröffentlicht: |
Dordrecht
Springer Netherlands
2012
|
| Schriftenreihe: | Advances in experimental medicine and biology
740 |
| In: |
Advances in experimental medicine and biology (740)
|
| Volumes / Articles: | Show Volumes / Articles. |
| DOI: | 10.1007/978-94-007-2888-2 |
| Schlagworte: | |
| Online-Zugang: | Verlag, Volltext: https://doi.org/10.1007/978-94-007-2888-2 Verlag, Volltext: http://dx.doi.org/10.1007/978-94-007-2888-2 Cover: https://swbplus.bsz-bw.de/bsz363415033cov.jpg 
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| Verfasserangaben: | edited by Md. Shahidul Islam |
MARC
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| 505 | 8 | |a Calcium Signaling; Preface; Contents; Chapter 1: The Regulation of a Cell's Ca 2+ Signaling Toolkit: The Ca 2+ Homeostasome; Components of the Calcium Signaling Toolkit with a Special Focus on Ca 2+ -Binding Proteins (CaBP) Classified as "Ca 2+ Buffers"; General Considerations; Ca 2+ Buffers/Ca 2+ Sensors Are Components of the Ca 2+ Homeostasome; Adaptation/Compensation/Homeostatic Mechanisms; General Considerations; Temporally Overlapping Activities of Ca 2+ Signaling Toolkit Components Impede the Precise Determination of Their Contribution to Intra- and Intercellular Ca 2+ Signaling | |
| 505 | 8 | |a Cellular Modifications Caused by Ablation/Blocking of a Ca 2+ Signaling Toolkit ComponentThe Ca 2+ Homeostasome; The Cellular Ca 2+ Homeostasome; The "Global" Ca 2+ Homeostasome; Conclusions, Hypotheses and Outlook; Note added in proof; References; Chapter 2: Methods to Detect Ca 2+ in Living Cells; Methods to Measure [Ca 2+ ] i Before the Era of Fluorescent Ca 2+ Indicators; Fluorescent Ca 2+ Indicators; How Does One Decide on Which Ca 2+ Indicator to Use?; How Does One Introduce Indicators into Cells?; Equipment Overview; Calibration; Future Directions; References | |
| 505 | 8 | |a Chapter 3: Development and Optimization of FLIPR High Throughput Calcium Assays for Ion Channels and GPCRsIntroduction; Calcium - A Universal Signaling Molecule; GPCRs; Ca 2+ -Permeable Ion Channels; Calcium Assays; Chemical Ca 2+ Indicator Dyes; Ratiometric Dyes; Quin-2; Fura-2; Bis-Fura-2; Fura-4F, Fura-5F, Fura-6F and Fura-FF; Fura-PE3 (Fura-2 LeakRes); FFP18; Fura-Red; Mag-Fura-2; Indo-1; Indo-1-PE3 (Indo-1 LeakRes); Mag-Indo-1; Single Wavelength Dyes; Fluo-3; Fluo-4; Mag-Fluo-4; Fluo-5F, Fluo-5Cl, Fluo-5N and Fluo-4FF; Fluo-8, Fluo-8H and Fluo-8L | |
| 505 | 8 | |a Rhod-2, X-Rhod-1 and Low Affinity DerivativesCalcium-Green-1 and Calcium-Green-2 Indicators; Oregon Green 488 BAPTA Indicators; Calcium Crimson, Calcium Ruby and Calcium Orange; Dye Loading; AM Ester Loading Technique; No-Wash Extracellular Quenchers; Problems with AM Ester Loading; FLIPR; Excitation Optics; Emission Optics; Emission Filters; Fluidics; Plate Stage; Software and Optional Accessories; Materials and Methods; Design and Optimization of FLIPR Assays; Agonists; Materials; Methods; PDL Coating; Cell Plating; Reagent Plate Preparation; Dye Preparation; Loading Cells with Dye | |
| 505 | 8 | |a Assay on FLIPR TETRA (Table 3.4)Applications; Examples; a 1B Adrenoreceptor Transiently Expressed in Cos-1 Cells (Figs. 3.2 and 3.3); Transfection; Cell Plating; Dye Loading; FLIPR Assay; Data Analysis; PAR2 Endogenously Expressed in HEK293 Cells (Fig. 3.4); Cell Plating; Dye Loading; FLIPR Assay; Data Analysis; a 7 nAChR Endogenously Expressed in SH-SY5Y Cells (Fig 3.5); Cell Plating; Dye Loading; FLIPR Assay; Data Analysis; References; Chapter 4: Two-Photon Calcium Imaging in the Intact Brain; Introduction; Two-Photon Microscopy for In Vivo Fluorescence Imaging | |
| 505 | 8 | |a Fluorescent Calcium Indicators | |
| 520 | |a Md. Shahidul Islam | ||
| 520 | |a Calcium signaling contains a unique selection of chapters that cover a wide range of contemporary topics in this ubiquitous and diverse system of cell signaling. This book has the flavor of a primary text book, but it is much more than that. It covers topics ranging from the fundamental aspects of calcium signaling to its clinical implications, in a thoughtful and comprehensive way. It discusses cutting edge researches, and critical issues at depth, and it presents many testable hypotheses for future research. It includes the theoretical and the methodological topics as well as topics related to mathematical modeling, and simulations. If you want to read about calcium signaling in different mammalian cells, oocytes, Zebrafishes, and even in plants, in one and the same book, then this book will not disappoint you. From the beginners to the experts in the field of calcium signaling, everybody will find something useful in this very timely book. | ||
| 650 | 0 | |a Biomedicine | |
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