Calcium signaling
Md. Shahidul Islam
Gespeichert in:
| Weitere Verfasser: | |
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| Dokumenttyp: | Book/Monograph |
| Sprache: | Englisch |
| Veröffentlicht: |
Dordrecht
Springer Netherlands
2012
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| Schriftenreihe: | Advances in experimental medicine and biology
740 |
| In: |
Advances in experimental medicine and biology (740)
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| Volumes / Articles: | Show Volumes / Articles. |
| DOI: | 10.1007/978-94-007-2888-2 |
| Schlagworte: | |
| Online-Zugang: | Verlag, Volltext: https://doi.org/10.1007/978-94-007-2888-2 Verlag, Volltext: http://dx.doi.org/10.1007/978-94-007-2888-2 Cover: https://swbplus.bsz-bw.de/bsz363415033cov.jpg 
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| Verfasserangaben: | edited by Md. Shahidul Islam |
Inhaltsangabe:
- Calcium Signaling; Preface; Contents; Chapter 1: The Regulation of a Cell's Ca 2+ Signaling Toolkit: The Ca 2+ Homeostasome; Components of the Calcium Signaling Toolkit with a Special Focus on Ca 2+ -Binding Proteins (CaBP) Classified as "Ca 2+ Buffers"; General Considerations; Ca 2+ Buffers/Ca 2+ Sensors Are Components of the Ca 2+ Homeostasome; Adaptation/Compensation/Homeostatic Mechanisms; General Considerations; Temporally Overlapping Activities of Ca 2+ Signaling Toolkit Components Impede the Precise Determination of Their Contribution to Intra- and Intercellular Ca 2+ Signaling
- Cellular Modifications Caused by Ablation/Blocking of a Ca 2+ Signaling Toolkit ComponentThe Ca 2+ Homeostasome; The Cellular Ca 2+ Homeostasome; The "Global" Ca 2+ Homeostasome; Conclusions, Hypotheses and Outlook; Note added in proof; References; Chapter 2: Methods to Detect Ca 2+ in Living Cells; Methods to Measure [Ca 2+ ] i Before the Era of Fluorescent Ca 2+ Indicators; Fluorescent Ca 2+ Indicators; How Does One Decide on Which Ca 2+ Indicator to Use?; How Does One Introduce Indicators into Cells?; Equipment Overview; Calibration; Future Directions; References
- Chapter 3: Development and Optimization of FLIPR High Throughput Calcium Assays for Ion Channels and GPCRsIntroduction; Calcium - A Universal Signaling Molecule; GPCRs; Ca 2+ -Permeable Ion Channels; Calcium Assays; Chemical Ca 2+ Indicator Dyes; Ratiometric Dyes; Quin-2; Fura-2; Bis-Fura-2; Fura-4F, Fura-5F, Fura-6F and Fura-FF; Fura-PE3 (Fura-2 LeakRes); FFP18; Fura-Red; Mag-Fura-2; Indo-1; Indo-1-PE3 (Indo-1 LeakRes); Mag-Indo-1; Single Wavelength Dyes; Fluo-3; Fluo-4; Mag-Fluo-4; Fluo-5F, Fluo-5Cl, Fluo-5N and Fluo-4FF; Fluo-8, Fluo-8H and Fluo-8L
- Rhod-2, X-Rhod-1 and Low Affinity DerivativesCalcium-Green-1 and Calcium-Green-2 Indicators; Oregon Green 488 BAPTA Indicators; Calcium Crimson, Calcium Ruby and Calcium Orange; Dye Loading; AM Ester Loading Technique; No-Wash Extracellular Quenchers; Problems with AM Ester Loading; FLIPR; Excitation Optics; Emission Optics; Emission Filters; Fluidics; Plate Stage; Software and Optional Accessories; Materials and Methods; Design and Optimization of FLIPR Assays; Agonists; Materials; Methods; PDL Coating; Cell Plating; Reagent Plate Preparation; Dye Preparation; Loading Cells with Dye
- Assay on FLIPR TETRA (Table 3.4)Applications; Examples; a 1B Adrenoreceptor Transiently Expressed in Cos-1 Cells (Figs. 3.2 and 3.3); Transfection; Cell Plating; Dye Loading; FLIPR Assay; Data Analysis; PAR2 Endogenously Expressed in HEK293 Cells (Fig. 3.4); Cell Plating; Dye Loading; FLIPR Assay; Data Analysis; a 7 nAChR Endogenously Expressed in SH-SY5Y Cells (Fig 3.5); Cell Plating; Dye Loading; FLIPR Assay; Data Analysis; References; Chapter 4: Two-Photon Calcium Imaging in the Intact Brain; Introduction; Two-Photon Microscopy for In Vivo Fluorescence Imaging
- Fluorescent Calcium Indicators