Necrotic cell debris induces a NF-κB-driven inflammasome response in vascular smooth muscle cells derived from abdominal aortic aneurysms (AAA-SMC)
Abdominal aortic aneurysm (AAA) is a multi-factorial progressive vascular disease with life-threatening complications. Increasing evidence suggests that smooth muscle cell (SMC) dysfunction and cell death contribute to dilatation and rupture of the aorta by inducing an inflammatory response. The exa...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
16 February 2019
|
| In: |
Biochemical and biophysical research communications
Year: 2019, Volume: 511, Issue: 2, Pages: 343-349 |
| ISSN: | 1090-2104 |
| DOI: | 10.1016/j.bbrc.2019.02.051 |
| Online Access: | Verlag, Volltext: https://doi.org/10.1016/j.bbrc.2019.02.051 Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S0006291X19302384 
|
| Author Notes: | Markus Wortmann, Ekaterina Skorubskaya, Andreas S. Peters, Maani Hakimi, Dittmar Böckler, Susanne Dihlmann |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1662537182 | ||
| 003 | DE-627 | ||
| 005 | 20240323101130.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 190329s2019 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.bbrc.2019.02.051 |2 doi | |
| 035 | |a (DE-627)1662537182 | ||
| 035 | |a (DE-599)KXP1662537182 | ||
| 035 | |a (OCoLC)1341205815 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Wortmann, Markus |d 1987- |e VerfasserIn |0 (DE-588)1077045328 |0 (DE-627)835863344 |0 (DE-576)445886722 |4 aut | |
| 245 | 1 | 0 | |a Necrotic cell debris induces a NF-κB-driven inflammasome response in vascular smooth muscle cells derived from abdominal aortic aneurysms (AAA-SMC) |c Markus Wortmann, Ekaterina Skorubskaya, Andreas S. Peters, Maani Hakimi, Dittmar Böckler, Susanne Dihlmann |
| 264 | 1 | |c 16 February 2019 | |
| 300 | |a 7 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 29.03.2019 | ||
| 520 | |a Abdominal aortic aneurysm (AAA) is a multi-factorial progressive vascular disease with life-threatening complications. Increasing evidence suggests that smooth muscle cell (SMC) dysfunction and cell death contribute to dilatation and rupture of the aorta by inducing an inflammatory response. The exact mechanism of this response however, is incompletely understood. We here investigated in vitro the capacity of autologous necrotic cell debris (CD) to induce inflammasome components and inflammatory mediators in aortic SMC (AAA-SMC) isolated from patients with AAA undergoing surgical repair. AAA-SMCs were additionally primed with Interferon- γ (IFN-γ) before treatment with CD in order to mimic the proinflammatory status caused by higher IFN-γ concentrations that have been demonstrated in the wall of AAAs. Real-time RT-PCR revealed that CD significantly increased NLRP3 and IL1B mRNA expression in different SMC cultures within 6h of exposure. Priming of the AAA-SMC with IFN-γ significantly increased expression of NLRP3, AIM2, IFI16 and CASP1 mRNAs, whereas IL1B mRNA was reduced. Additional exposure of IFN-γ-primed AAA-SMC to CD for 6-24h, further augmented expression of AIM2, NLRP3, and Caspase-1 protein levels. Analysis of the SMC supernatants by ELISA revealed CD-induced release of the senescence-associated cytokines IL-6 and MCP-1 in native and IFN-γ-primed SMC, whereas no secretion of Interleukin-(IL) 1α and IL-1β secretion were observed. Our results implicate a role of necrotic cell debris derived from dead neighboring cells in SMC dysfunction and in inflammatory response of AAA tissue. | ||
| 650 | 4 | |a Abdominal aortic aneurysm | |
| 650 | 4 | |a AIM2 | |
| 650 | 4 | |a Inflammasome | |
| 650 | 4 | |a Necrosis | |
| 650 | 4 | |a NLRP3 | |
| 650 | 4 | |a VSMC | |
| 700 | 1 | |a Peters, Andreas |d 1982- |e VerfasserIn |0 (DE-588)143674587 |0 (DE-627)704565943 |0 (DE-576)338312617 |4 aut | |
| 700 | 1 | |a Hakimi, Maani |d 1972- |e VerfasserIn |0 (DE-588)130842494 |0 (DE-627)506359727 |0 (DE-576)298370891 |4 aut | |
| 700 | 1 | |a Böckler, Dittmar |d 1966- |e VerfasserIn |0 (DE-588)1032367881 |0 (DE-627)738382868 |0 (DE-576)173503969 |4 aut | |
| 700 | 1 | |a Dihlmann, Susanne |d 1966- |e VerfasserIn |0 (DE-588)114711054 |0 (DE-627)530925842 |0 (DE-576)289805821 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Biochemical and biophysical research communications |d [Amsterdam] : Elsevier B.V., 1959 |g 511(2019), 2, Seite 343-349 |h Online-Ressource |w (DE-627)254231691 |w (DE-600)1461396-7 |w (DE-576)103373039 |x 1090-2104 |7 nnas |a Necrotic cell debris induces a NF-κB-driven inflammasome response in vascular smooth muscle cells derived from abdominal aortic aneurysms (AAA-SMC) |
| 773 | 1 | 8 | |g volume:511 |g year:2019 |g number:2 |g pages:343-349 |g extent:7 |a Necrotic cell debris induces a NF-κB-driven inflammasome response in vascular smooth muscle cells derived from abdominal aortic aneurysms (AAA-SMC) |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.bbrc.2019.02.051 |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u http://www.sciencedirect.com/science/article/pii/S0006291X19302384 |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20190329 | ||
| 993 | |a Article | ||
| 994 | |a 2019 | ||
| 998 | |g 114711054 |a Dihlmann, Susanne |m 114711054:Dihlmann, Susanne |d 910000 |d 910200 |e 910000PD114711054 |e 910200PD114711054 |k 0/910000/ |k 1/910000/910200/ |p 6 |y j | ||
| 998 | |g 1032367881 |a Böckler, Dittmar |m 1032367881:Böckler, Dittmar |d 910000 |d 910200 |e 910000PB1032367881 |e 910200PB1032367881 |k 0/910000/ |k 1/910000/910200/ |p 5 | ||
| 998 | |g 130842494 |a Hakimi, Maani |m 130842494:Hakimi, Maani |d 910000 |d 910200 |e 910000PH130842494 |e 910200PH130842494 |k 0/910000/ |k 1/910000/910200/ |p 4 | ||
| 998 | |g 143674587 |a Peters, Andreas |m 143674587:Peters, Andreas |d 910000 |d 910200 |e 910000PP143674587 |e 910200PP143674587 |k 0/910000/ |k 1/910000/910200/ |p 3 | ||
| 998 | |g 1077045328 |a Wortmann, Markus |m 1077045328:Wortmann, Markus |d 910000 |d 910200 |e 910000PW1077045328 |e 910200PW1077045328 |k 0/910000/ |k 1/910000/910200/ |p 1 |x j | ||
| 999 | |a KXP-PPN1662537182 |e 3417380804 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"name":{"displayForm":["Markus Wortmann, Ekaterina Skorubskaya, Andreas S. Peters, Maani Hakimi, Dittmar Böckler, Susanne Dihlmann"]},"language":["eng"],"note":["Gesehen am 29.03.2019"],"recId":"1662537182","person":[{"given":"Markus","display":"Wortmann, Markus","role":"aut","family":"Wortmann"},{"role":"aut","family":"Peters","display":"Peters, Andreas","given":"Andreas"},{"display":"Hakimi, Maani","given":"Maani","family":"Hakimi","role":"aut"},{"role":"aut","family":"Böckler","display":"Böckler, Dittmar","given":"Dittmar"},{"role":"aut","family":"Dihlmann","given":"Susanne","display":"Dihlmann, Susanne"}],"physDesc":[{"extent":"7 S."}],"id":{"eki":["1662537182"],"doi":["10.1016/j.bbrc.2019.02.051"]},"title":[{"title":"Necrotic cell debris induces a NF-κB-driven inflammasome response in vascular smooth muscle cells derived from abdominal aortic aneurysms (AAA-SMC)","title_sort":"Necrotic cell debris induces a NF-κB-driven inflammasome response in vascular smooth muscle cells derived from abdominal aortic aneurysms (AAA-SMC)"}],"type":{"media":"Online-Ressource","bibl":"article-journal"},"origin":[{"dateIssuedKey":"2019","dateIssuedDisp":"16 February 2019"}],"relHost":[{"titleAlt":[{"title":"BBRC"}],"type":{"media":"Online-Ressource","bibl":"periodical"},"disp":"Necrotic cell debris induces a NF-κB-driven inflammasome response in vascular smooth muscle cells derived from abdominal aortic aneurysms (AAA-SMC)Biochemical and biophysical research communications","note":["Gesehen am 14.06.2024"],"pubHistory":["1.1959 -"],"part":{"extent":"7","pages":"343-349","issue":"2","text":"511(2019), 2, Seite 343-349","year":"2019","volume":"511"},"language":["eng"],"origin":[{"dateIssuedDisp":"1959-","publisher":"Elsevier B.V. ; Academic Press","publisherPlace":"[Amsterdam] ; Orlando, Fla.","dateIssuedKey":"1959"}],"id":{"zdb":["1461396-7"],"issn":["1090-2104"],"eki":["254231691"]},"physDesc":[{"extent":"Online-Ressource"}],"title":[{"title_sort":"Biochemical and biophysical research communications","title":"Biochemical and biophysical research communications","subtitle":"BBRC"}],"recId":"254231691"}]} | ||
| SRT | |a WORTMANNMANECROTICCE1620 | ||