Cover Image

The intramembrane protease SPP impacts morphology of the endoplasmic reticulum by triggering degradation of morphogenic proteins

The endoplasmic reticulum (ER), as a multifunctional organelle, plays crucial roles in lipid biosynthesis and calcium homeostasis as well as the synthesis and folding of secretory and membrane proteins. Therefore, it is of high importance to maintain ER homeostasis and to adapt ER function and morph...

Full description

Saved in:
Bibliographic Details
Main Authors: Avci, Dönem (Author) , Malchus, Nicole Susanne (Author) , Heidasch, Ronny (Author) , Lorenz, Holger (Author) , Richter, Karsten (Author) , Neßling, Michelle (Author) , Lemberg, Marius (Author)
Format: Article (Journal)
Language:English
Published: 2019
In: The journal of biological chemistry
Year: 2018, Volume: 294, Issue: 8, Pages: 2786-2800
ISSN:1083-351X
DOI:10.1074/jbc.RA118.005642
Online Access:Verlag, Volltext: https://doi.org/10.1074/jbc.RA118.005642
Verlag, Volltext: http://www.jbc.org/content/294/8/2786
Get full text
Author Notes:Dönem Avci, Nicole S. Malchus, Ronny Heidasch, Holger Lorenz, Karsten Richter, Michelle Neßling, and Marius K. Lemberg

MARC

LEADER 00000caa a2200000 c 4500
001 166325821X
003 DE-627
005 20220816134452.0
007 cr uuu---uuuuu
008 190415r20192018xx |||||o 00| ||eng c
024 7 |a 10.1074/jbc.RA118.005642  |2 doi 
035 |a (DE-627)166325821X 
035 |a (DE-599)KXP166325821X 
035 |a (OCoLC)1341209644 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 32  |2 sdnb 
100 1 |a Avci, Dönem  |e VerfasserIn  |0 (DE-588)1034783394  |0 (DE-627)746277199  |0 (DE-576)382417135  |4 aut 
245 1 4 |a The intramembrane protease SPP impacts morphology of the endoplasmic reticulum by triggering degradation of morphogenic proteins  |c Dönem Avci, Nicole S. Malchus, Ronny Heidasch, Holger Lorenz, Karsten Richter, Michelle Neßling, and Marius K. Lemberg 
264 1 |c 2019 
300 |a 15 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a First published on December 21, 2018 
500 |a Gesehen am 15.04.2019 
520 |a The endoplasmic reticulum (ER), as a multifunctional organelle, plays crucial roles in lipid biosynthesis and calcium homeostasis as well as the synthesis and folding of secretory and membrane proteins. Therefore, it is of high importance to maintain ER homeostasis and to adapt ER function and morphology to cellular needs. Here, we show that signal peptide peptidase (SPP) modulates the ER shape through degradation of morphogenic proteins. Elevating SPP activity induces rapid rearrangement of the ER and formation of dynamic ER clusters. Inhibition of SPP activity rescues the phenotype without the need for new protein synthesis, and this rescue depends on a pre-existing pool of proteins in the Golgi. With the help of organelle proteomics, we identified certain membrane proteins to be diminished upon SPP expression and further show that the observed morphology changes depend on SPP-mediated cleavage of ER morphogenic proteins, including the SNARE protein syntaxin-18. Thus, we suggest that SPP-mediated protein abundance control by a regulatory branch of ER-associated degradation (ERAD-R) has a role in shaping the early secretory pathway. 
534 |c 2018 
650 4 |a cellular regulation 
650 4 |a endoplasmic reticulum-associated protein degradation (ERAD) 
650 4 |a intramembrane proteolysis 
650 4 |a organelle 
650 4 |a organelle morphology 
650 4 |a proteolysis 
650 4 |a substrate identification 
650 4 |a tail-anchored protein 
700 1 |a Malchus, Nicole Susanne  |e VerfasserIn  |0 (DE-588)1082259977  |0 (DE-627)847268071  |0 (DE-576)455299331  |4 aut 
700 1 |a Heidasch, Ronny  |d 1988-  |e VerfasserIn  |0 (DE-588)1183630212  |0 (DE-627)1663260222  |4 aut 
700 1 |a Lorenz, Holger  |d 1971-  |e VerfasserIn  |0 (DE-588)123660424  |0 (DE-627)706385497  |0 (DE-576)293813450  |4 aut 
700 1 |a Richter, Karsten  |e VerfasserIn  |0 (DE-588)1184444447  |0 (DE-627)1663571104  |4 aut 
700 1 |a Neßling, Michelle  |d 1971-  |e VerfasserIn  |0 (DE-588)123316340  |0 (DE-627)706253159  |0 (DE-576)293651884  |4 aut 
700 1 |a Lemberg, Marius  |e VerfasserIn  |0 (DE-588)1063794293  |0 (DE-627)812604490  |0 (DE-576)422912328  |4 aut 
773 0 8 |i Enthalten in  |t The journal of biological chemistry  |d [Amsterdam] : Elsevier B.V., 1905  |g 294(2019), 8, Seite 2786-2800  |h Online-Ressource  |w (DE-627)269247025  |w (DE-600)1474604-9  |w (DE-576)077883837  |x 1083-351X  |7 nnas  |a The intramembrane protease SPP impacts morphology of the endoplasmic reticulum by triggering degradation of morphogenic proteins 
773 1 8 |g volume:294  |g year:2019  |g number:8  |g pages:2786-2800  |g extent:15  |a The intramembrane protease SPP impacts morphology of the endoplasmic reticulum by triggering degradation of morphogenic proteins 
856 4 0 |u https://doi.org/10.1074/jbc.RA118.005642  |x Verlag  |x Resolving-System  |3 Volltext 
856 4 0 |u http://www.jbc.org/content/294/8/2786  |x Verlag  |3 Volltext 
951 |a AR 
992 |a 20190415 
993 |a Article 
994 |a 2019 
998 |g 1063794293  |a Lemberg, Marius  |m 1063794293:Lemberg, Marius  |d 700000  |d 706000  |e 700000PL1063794293  |e 706000PL1063794293  |k 0/700000/  |k 1/700000/706000/  |p 7  |y j 
998 |g 1184444447  |a Richter, Karsten  |m 1184444447:Richter, Karsten  |d 140000  |e 140000PR1184444447  |k 0/140000/  |p 5 
998 |g 123660424  |a Lorenz, Holger  |m 123660424:Lorenz, Holger  |d 700000  |d 706000  |e 700000PL123660424  |e 706000PL123660424  |k 0/700000/  |k 1/700000/706000/  |p 4 
998 |g 1183630212  |a Heidasch, Ronny  |m 1183630212:Heidasch, Ronny  |d 700000  |d 706000  |e 700000PH1183630212  |e 706000PH1183630212  |k 0/700000/  |k 1/700000/706000/  |p 3 
998 |g 1082259977  |a Malchus, Nicole Susanne  |m 1082259977:Malchus, Nicole Susanne  |p 2 
998 |g 1034783394  |a Avci, Dönem  |m 1034783394:Avci, Dönem  |d 700000  |d 706000  |e 700000PA1034783394  |e 706000PA1034783394  |k 0/700000/  |k 1/700000/706000/  |p 1  |x j 
999 |a KXP-PPN166325821X  |e 3422451269 
BIB |a Y 
SER |a journal 
JSO |a {"title":[{"title_sort":"intramembrane protease SPP impacts morphology of the endoplasmic reticulum by triggering degradation of morphogenic proteins","title":"The intramembrane protease SPP impacts morphology of the endoplasmic reticulum by triggering degradation of morphogenic proteins"}],"name":{"displayForm":["Dönem Avci, Nicole S. Malchus, Ronny Heidasch, Holger Lorenz, Karsten Richter, Michelle Neßling, and Marius K. Lemberg"]},"type":{"media":"Online-Ressource","bibl":"article-journal"},"physDesc":[{"extent":"15 S."}],"recId":"166325821X","language":["eng"],"note":["First published on December 21, 2018","Gesehen am 15.04.2019"],"person":[{"family":"Avci","given":"Dönem","roleDisplay":"VerfasserIn","role":"aut","display":"Avci, Dönem"},{"roleDisplay":"VerfasserIn","role":"aut","display":"Malchus, Nicole Susanne","given":"Nicole Susanne","family":"Malchus"},{"roleDisplay":"VerfasserIn","role":"aut","display":"Heidasch, Ronny","given":"Ronny","family":"Heidasch"},{"role":"aut","roleDisplay":"VerfasserIn","display":"Lorenz, Holger","family":"Lorenz","given":"Holger"},{"display":"Richter, Karsten","roleDisplay":"VerfasserIn","role":"aut","family":"Richter","given":"Karsten"},{"given":"Michelle","family":"Neßling","roleDisplay":"VerfasserIn","role":"aut","display":"Neßling, Michelle"},{"given":"Marius","family":"Lemberg","display":"Lemberg, Marius","roleDisplay":"VerfasserIn","role":"aut"}],"id":{"doi":["10.1074/jbc.RA118.005642"],"eki":["166325821X"]},"origin":[{"dateIssuedKey":"2019","dateIssuedDisp":"2019"}],"relHost":[{"physDesc":[{"extent":"Online-Ressource"}],"language":["eng"],"corporate":[{"roleDisplay":"Herausgebendes Organ","role":"isb","display":"American Society for Biochemistry and Molecular Biology"}],"recId":"269247025","note":["Gesehen am 19.05.2021"],"type":{"bibl":"periodical","media":"Online-Ressource"},"disp":"The intramembrane protease SPP impacts morphology of the endoplasmic reticulum by triggering degradation of morphogenic proteinsThe journal of biological chemistry","id":{"eki":["269247025"],"issn":["1083-351X"],"zdb":["1474604-9"]},"origin":[{"dateIssuedDisp":"2021-","publisher":"Elsevier B.V. ; American Soc. of Biological Chemists ; ASBMB Publications","publisherPlace":"[Amsterdam] ; Baltimore, Md. [u.a.] ; Bethesda, Md.","dateIssuedKey":"2021"}],"part":{"year":"2019","extent":"15","text":"294(2019), 8, Seite 2786-2800","issue":"8","volume":"294","pages":"2786-2800"},"title":[{"subtitle":"JBC","title":"The journal of biological chemistry","title_sort":"journal of biological chemistry"}],"name":{"displayForm":["publ. by the American Society for Biochemistry and Molecular Biology"]},"pubHistory":["1.1905/06 -"],"titleAlt":[{"title":"JBC online"},{"title":"JBC"}]}]} 
SRT |a AVCIDOENEMINTRAMEMBR2019 

Similar Items