Buchumschlag

Overexpression of calretinin enhances short-term synaptic depression

Analysis of the effects of various proteins on short-term synaptic plasticity is a difficult task, which may require the use of knockout animals. Here, we propose alternative experimental approach for studying the roles of desired proteins in synaptic plasticity. We packed Ca2+-binding protein calre...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Bolshakov, Alexey P. (VerfasserIn) , Rozov, Andrei (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 13 March 2019
In: Frontiers in cellular neuroscience
Year: 2019, Jahrgang: 13, Pages: 91
ISSN:1662-5102
DOI:10.3389/fncel.2019.00091
Online-Zugang:Verlag, Volltext: https://doi.org/10.3389/fncel.2019.00091
Verlag, Volltext: https://www.frontiersin.org/articles/10.3389/fncel.2019.00091/full
Volltext
Verfasserangaben:Alexey P. Bolshakov, Alexander Kolleker, Evgenia P. Volkova, Fliza Valiullina-Rakhmatullina, Peter M. Kolosov, Andrei Rozov

MARC

LEADER 00000caa a2200000 c 4500
001 1663301506
003 DE-627
005 20220816134949.0
007 cr uuu---uuuuu
008 190416s2019 xx |||||o 00| ||eng c
024 7 |a 10.3389/fncel.2019.00091  |2 doi 
035 |a (DE-627)1663301506 
035 |a (DE-599)KXP1663301506 
035 |a (OCoLC)1341209673 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 33  |2 sdnb 
100 1 |a Bolshakov, Alexey P.  |e VerfasserIn  |0 (DE-588)1183724055  |0 (DE-627)1663303029  |4 aut 
245 1 0 |a Overexpression of calretinin enhances short-term synaptic depression  |c Alexey P. Bolshakov, Alexander Kolleker, Evgenia P. Volkova, Fliza Valiullina-Rakhmatullina, Peter M. Kolosov, Andrei Rozov 
264 1 |c 13 March 2019 
300 |a 7 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Gesehen am 16.04.2019 
520 |a Analysis of the effects of various proteins on short-term synaptic plasticity is a difficult task, which may require the use of knockout animals. Here, we propose alternative experimental approach for studying the roles of desired proteins in synaptic plasticity. We packed Ca2+-binding protein calretinin and fluorescent protein Venus into AAV and injected the concentrated viral suspension into the neocortex of newborn rats. The infected layer 2/3 pyramidal cells were identified in rat cortical slices using Venus fluorescence. Analysis of short-term synaptic plasticity using paired patch clamp recordings between layer 2/3 pyramidal cells (presynaptic cell) and fast-spiking interneurons (postsynaptic cell) showed that calretinin expression in the pyramidal cells did not change the failure rate in this synapse but did decrease synaptic delay. Analysis of the parameters of short-term synaptic plasticity showed that the amplitude of the first EPSP in the train was not affected by calretinin, however calretinin strongly enhanced short-term depression. In addition, we found that the effect of calretinin depended on the presynaptic firing frequency - an increase in frequency resulted in enhancement of synaptic depression. 
650 4 |a calretenin 
650 4 |a interneuron 
650 4 |a Neocortex 
650 4 |a Pyramidal Cells 
650 4 |a release probability 
650 4 |a viral expression 
700 1 |a Rozov, Andrei  |d 1968-  |e VerfasserIn  |0 (DE-588)122769686  |0 (DE-627)706036190  |0 (DE-576)293413312  |4 aut 
773 0 8 |i Enthalten in  |t Frontiers in cellular neuroscience  |d Lausanne : Frontiers Research Foundation, 2007  |g 13(2019) Artikel-Nummer 91, 7 Seiten  |h Online-Ressource  |w (DE-627)579826414  |w (DE-600)2452963-1  |w (DE-576)286502240  |x 1662-5102  |7 nnas  |a Overexpression of calretinin enhances short-term synaptic depression 
773 1 8 |g volume:13  |g year:2019  |g pages:91  |g extent:7  |a Overexpression of calretinin enhances short-term synaptic depression 
856 4 0 |u https://doi.org/10.3389/fncel.2019.00091  |x Verlag  |x Resolving-System  |3 Volltext 
856 4 0 |u https://www.frontiersin.org/articles/10.3389/fncel.2019.00091/full  |x Verlag  |3 Volltext 
951 |a AR 
992 |a 20190416 
993 |a Article 
994 |a 2019 
998 |g 122769686  |a Rozov, Andrei  |m 122769686:Rozov, Andrei  |d 50000  |d 53200  |e 50000PR122769686  |e 53200PR122769686  |k 0/50000/  |k 1/50000/53200/  |p 6  |y j 
999 |a KXP-PPN1663301506  |e 342257705X 
BIB |a Y 
SER |a journal 
JSO |a {"relHost":[{"id":{"eki":["579826414"],"zdb":["2452963-1"],"issn":["1662-5102"]},"origin":[{"publisherPlace":"Lausanne","dateIssuedDisp":"2007-","dateIssuedKey":"2007","publisher":"Frontiers Research Foundation"}],"name":{"displayForm":["Frontiers Research Foundation"]},"physDesc":[{"extent":"Online-Ressource"}],"title":[{"title":"Frontiers in cellular neuroscience","title_sort":"Frontiers in cellular neuroscience"}],"part":{"year":"2019","pages":"91","volume":"13","text":"13(2019) Artikel-Nummer 91, 7 Seiten","extent":"7"},"pubHistory":["2007 -"],"language":["eng"],"recId":"579826414","type":{"media":"Online-Ressource","bibl":"periodical"},"disp":"Overexpression of calretinin enhances short-term synaptic depressionFrontiers in cellular neuroscience","note":["Gesehen am 04.06.20"]}],"physDesc":[{"extent":"7 S."}],"name":{"displayForm":["Alexey P. Bolshakov, Alexander Kolleker, Evgenia P. Volkova, Fliza Valiullina-Rakhmatullina, Peter M. Kolosov, Andrei Rozov"]},"id":{"doi":["10.3389/fncel.2019.00091"],"eki":["1663301506"]},"origin":[{"dateIssuedDisp":"13 March 2019","dateIssuedKey":"2019"}],"language":["eng"],"recId":"1663301506","note":["Gesehen am 16.04.2019"],"type":{"media":"Online-Ressource","bibl":"article-journal"},"person":[{"given":"Alexey P.","family":"Bolshakov","role":"aut","roleDisplay":"VerfasserIn","display":"Bolshakov, Alexey P."},{"role":"aut","display":"Rozov, Andrei","roleDisplay":"VerfasserIn","given":"Andrei","family":"Rozov"}],"title":[{"title_sort":"Overexpression of calretinin enhances short-term synaptic depression","title":"Overexpression of calretinin enhances short-term synaptic depression"}]} 
SRT |a BOLSHAKOVAOVEREXPRES1320 

Ähnliche Einträge