Cover Image

Visualizing RNA in live bacterial cells using fluorophore- and quencher-binding aptamers

To elucidate the roles, dynamics, and regulation of RNAs, it is vital to be able to visualize the RNA of interest (ROI) in living cells noninvasively. Here, we describe a novel live-cell RNA imaging method using fluorophore- and quencher-binding aptamers, which can be genetically fused to the ROI. I...

Full description

Saved in:
Bibliographic Details
Main Authors: Sünbül, Murat (Author) , Arora, Ankita (Author) , Jäschke, Andres (Author)
Format: Chapter/Article
Language:English
Published: 2018
In: RNA Detection
Year: 2018, Pages: 289-304
DOI:10.1007/978-1-4939-7213-5_19
Online Access:Verlag, Volltext: https://doi.org/10.1007/978-1-4939-7213-5_19
Get full text
Author Notes:Murat Sunbul, Ankita Arora, Andres Jäschke

MARC

LEADER 00000caa a2200000 c 4500
001 1663702055
003 DE-627
005 20220816142418.0
007 cr uuu---uuuuu
008 190429s2018 xx |||||o 00| ||eng c
024 7 |a 10.1007/978-1-4939-7213-5_19  |2 doi 
035 |a (DE-627)1663702055 
035 |a (DE-599)KXP1663702055 
035 |a (OCoLC)1341211848 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 32  |2 sdnb 
100 1 |a Sünbül, Murat  |e VerfasserIn  |0 (DE-588)118481189X  |0 (DE-627)166370385X  |4 aut 
245 1 0 |a Visualizing RNA in live bacterial cells using fluorophore- and quencher-binding aptamers  |c Murat Sunbul, Ankita Arora, Andres Jäschke 
264 1 |c 2018 
300 |a 16 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Gesehen am 29.04.2019 
520 |a To elucidate the roles, dynamics, and regulation of RNAs, it is vital to be able to visualize the RNA of interest (ROI) in living cells noninvasively. Here, we describe a novel live-cell RNA imaging method using fluorophore- and quencher-binding aptamers, which can be genetically fused to the ROI. In this method, new membrane permeable and nonfluorescent fluorophore-quencher conjugates were utilized, and we showed that their fluorescence increases dramatically upon binding to fluorophore- or quencher-binding aptamers. This phenomenon allowed for labeling the ROI with many different colored fluorophores and also dual-color imaging of two distinct RNAs in live bacteria. Our approach uses small RNA tags and small molecule fluorophores for labeling, thereby minimal perturbation on the function and dynamics of the RNA of interest is expected. 
650 4 |a Aptamer 
650 4 |a Contact quenching 
650 4 |a DNB aptamer 
650 4 |a Fluorescence microscopy 
650 4 |a Fluorophore 
650 4 |a Live cell imaging 
650 4 |a RNA imaging 
650 4 |a RNA localization 
650 4 |a RNA trafficking 
650 4 |a SRB-2 aptamer 
700 1 |a Arora, Ankita  |e VerfasserIn  |0 (DE-588)1081430591  |0 (DE-627)846108291  |0 (DE-576)45440221X  |4 aut 
700 1 |a Jäschke, Andres  |d 1962-  |e VerfasserIn  |0 (DE-588)1025112261  |0 (DE-627)751682756  |0 (DE-576)170797481  |4 aut 
773 0 8 |i Enthalten in  |t RNA Detection  |d New York, NY : Humana Press, 2018  |g (2018), Seite 289-304  |h Online-Ressource (XIV, 492 p. 94 illus., 78 illus. in color, online resource)  |w (DE-627)165720314X  |w (DE-576)495982733  |z 9781493972135  |7 nnam  |a Visualizing RNA in live bacterial cells using fluorophore- and quencher-binding aptamers 
773 1 8 |g year:2018  |g pages:289-304  |g extent:16  |a Visualizing RNA in live bacterial cells using fluorophore- and quencher-binding aptamers 
856 4 0 |u https://doi.org/10.1007/978-1-4939-7213-5_19  |x Verlag  |x Resolving-System  |3 Volltext 
951 |a AR 
992 |a 20190429 
993 |a BookComponentPart 
994 |a 2018 
998 |g 1025112261  |a Jäschke, Andres  |m 1025112261:Jäschke, Andres  |d 160000  |d 160100  |d 700000  |d 718000  |e 160000PJ1025112261  |e 160100PJ1025112261  |e 700000PJ1025112261  |e 718000PJ1025112261  |k 0/160000/  |k 1/160000/160100/  |k 0/700000/  |k 1/700000/718000/  |p 3  |y j 
998 |g 1081430591  |a Arora, Ankita  |m 1081430591:Arora, Ankita  |d 160000  |d 160100  |e 160000PA1081430591  |e 160100PA1081430591  |k 0/160000/  |k 1/160000/160100/  |p 2 
998 |g 118481189X  |a Sünbül, Murat  |m 118481189X:Sünbül, Murat  |d 160000  |d 160100  |e 160000PS118481189X  |e 160100PS118481189X  |k 0/160000/  |k 1/160000/160100/  |p 1  |x j 
999 |a KXP-PPN1663702055  |e 345363120X 
BIB |a Y 
JSO |a {"physDesc":[{"extent":"16 S."}],"origin":[{"dateIssuedKey":"2018","dateIssuedDisp":"2018"}],"type":{"bibl":"chapter","media":"Online-Ressource"},"title":[{"title":"Visualizing RNA in live bacterial cells using fluorophore- and quencher-binding aptamers","title_sort":"Visualizing RNA in live bacterial cells using fluorophore- and quencher-binding aptamers"}],"language":["eng"],"name":{"displayForm":["Murat Sunbul, Ankita Arora, Andres Jäschke"]},"note":["Gesehen am 29.04.2019"],"id":{"eki":["1663702055"],"doi":["10.1007/978-1-4939-7213-5_19"]},"recId":"1663702055","relHost":[{"physDesc":[{"extent":"Online-Ressource (XIV, 492 p. 94 illus., 78 illus. in color, online resource)"}],"origin":[{"publisherPlace":"New York, NY","dateIssuedDisp":"2018","dateIssuedKey":"2018","publisher":"Humana Press"}],"type":{"media":"Online-Ressource","bibl":"edited-book"},"part":{"extent":"16","pages":"289-304","year":"2018","text":"(2018), Seite 289-304"},"relMultPart":[{"note":["Gesehen am 05.11.12"],"id":{"eki":["599671882"],"zdb":["2493551-7"],"issn":["1940-6029"]},"recId":"599671882","disp":"Methods in Molecular Biology","language":["eng"],"title":[{"title_sort":"Methods in molecular biology","title":"Methods in molecular biology"}],"type":{"media":"Online-Ressource","bibl":"serial"},"pubHistory":["1.1984 -"],"part":{"number_sort":["1649"],"number":["1649"]},"dispAlt":"Methods in molecular biology","physDesc":[{"extent":"Online-Ressource"}],"origin":[{"publisherPlace":"Totowa, NJ ; [Heidelberg]","dateIssuedKey":"1984","publisher":"Humana Press ; [Springer]","dateIssuedDisp":"1984-"}]}],"disp":"Visualizing RNA in live bacterial cells using fluorophore- and quencher-binding aptamersRNA Detection","title":[{"title_sort":"RNA Detection","title":"RNA Detection","subtitle":"Methods and Protocols"}],"language":["eng"],"name":{"displayForm":["edited by Imre Gaspar"]},"id":{"isbn":["9781493972135"],"doi":["10.1007/978-1-4939-7213-5"],"eki":["165720314X"]},"recId":"165720314X","person":[{"display":"Gaspar, Imre","family":"Gaspar","roleDisplay":"HerausgeberIn","role":"edt","given":"Imre"}]}],"person":[{"family":"Sünbül","display":"Sünbül, Murat","roleDisplay":"VerfasserIn","role":"aut","given":"Murat"},{"roleDisplay":"VerfasserIn","role":"aut","family":"Arora","display":"Arora, Ankita","given":"Ankita"},{"role":"aut","roleDisplay":"VerfasserIn","family":"Jäschke","display":"Jäschke, Andres","given":"Andres"}]} 
SRT |a SUENBUELMUVISUALIZIN2018 

Similar Items