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Random motion of chromatin is influenced by lamin a interconnections

Using fluorescence correlation spectroscopy in single-plane illumination microscopy, we investigated the dynamics of chromatin in interphase mouse adult fibroblast cell nuclei under the influence of the intermediate filament protein lamin A. We find that 1) lamin A-eGFP and histone H2A-mRFP show sig...

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Main Authors: Taheri, Fereydoon (Author) , Isbilir, Buse (Author) , Müller, Gabriele (Author) , Krieger, Jan Wolfgang (Author) , Chirico, Giuseppe (Author) , Langowski, Jörg (Author) , Tóth, Katalin (Author)
Format: Article (Journal)
Language:English
Published: 11 May 2018
In: Biophysical journal
Year: 2018, Volume: 114, Issue: 10, Pages: 2465-2472
ISSN:1542-0086
DOI:10.1016/j.bpj.2018.04.037
Online Access:Verlag, Volltext: https://doi.org/10.1016/j.bpj.2018.04.037
Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S0006349518305320
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Author Notes:Fereydoon Taheri, Buse Isbilir, Gabriele Müller, Jan W. Krieger, Giuseppe Chirico, Jörg Langowski, Katalin Tóth

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520 |a Using fluorescence correlation spectroscopy in single-plane illumination microscopy, we investigated the dynamics of chromatin in interphase mouse adult fibroblast cell nuclei under the influence of the intermediate filament protein lamin A. We find that 1) lamin A-eGFP and histone H2A-mRFP show significant comobility, indicating that their motions are clearly interconnected in the nucleus, and 2) that the random motion of histones H2A within the chromatin network is subdiffusive, i.e., the effective diffusion coefficient decreases for slow timescales. Knocking out lamin A changes the diffusion back to normal. Thus, lamin A influences the dynamics of the entire chromatin network. Our conclusion is that lamin A plays a central role in determining the viscoelasticity of the chromatin network and helping to maintain local ordering of interphase chromosomes. 
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