Functional proteomics of breast cancer metabolism identifies GLUL as responder during hypoxic adaptation
Hypoxia as well as metabolism are central hallmarks of cancer, and hypoxia-inducible factors (HIFs) and metabolic effectors are crucial elements in oxygen-compromised tumor environments. Knowledge of changes in the expression of metabolic proteins in response to HIF function could provide mechanisti...
Gespeichert in:
| Hauptverfasser: | , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
January 4, 2019
|
| In: |
Journal of proteome research
Year: 2019, Jahrgang: 18, Heft: 3, Pages: 1352-1362 |
| ISSN: | 1535-3907 |
| DOI: | 10.1021/acs.jproteome.8b00944 |
| Online-Zugang: | Verlag, Volltext: https://doi.org/10.1021/acs.jproteome.8b00944
|
| Verfasserangaben: | Stephan Bernhardt, Christian Tönsing, Devina Mitra, Nese Erdem, Karin Müller-Decker, Ulrike Korf, Clemens Kreutz, Jens Timmer, and Stefan Wiemann |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1665339624 | ||
| 003 | DE-627 | ||
| 005 | 20230427000815.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 190514s2019 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1021/acs.jproteome.8b00944 |2 doi | |
| 035 | |a (DE-627)1665339624 | ||
| 035 | |a (DE-599)KXP1665339624 | ||
| 035 | |a (OCoLC)1341213095 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Bernhardt, Stephan |e VerfasserIn |0 (DE-588)1147341044 |0 (DE-627)1006090363 |0 (DE-576)495806331 |4 aut | |
| 245 | 1 | 0 | |a Functional proteomics of breast cancer metabolism identifies GLUL as responder during hypoxic adaptation |c Stephan Bernhardt, Christian Tönsing, Devina Mitra, Nese Erdem, Karin Müller-Decker, Ulrike Korf, Clemens Kreutz, Jens Timmer, and Stefan Wiemann |
| 264 | 1 | |c January 4, 2019 | |
| 300 | |a 11 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 14.05.2019 | ||
| 520 | |a Hypoxia as well as metabolism are central hallmarks of cancer, and hypoxia-inducible factors (HIFs) and metabolic effectors are crucial elements in oxygen-compromised tumor environments. Knowledge of changes in the expression of metabolic proteins in response to HIF function could provide mechanistic insights into adaptation to hypoxic stress, tumorigenesis, and disease progression. We analyzed time-resolved alterations in metabolism-associated protein levels in response to different oxygen potentials across breast cancer cell lines. Effects on the cellular metabolism of both HIF-dependent and -independent processes were analyzed by reverse-phase protein array profiling and a custom statistical model. We revealed a strong induction of glucose transporter 1 (GLUT1) and lactate dehydrogenase A (LDHA) as well as reduced glutamate-ammonia ligase (GLUL) protein levels across all cell lines tested as consistent changes upon hypoxia induction. Low GLUL protein levels were correlated with aggressive molecular subtypes in breast cancer patient data sets and also with hypoxic tumor regions in a xenograft mouse tumor model. Moreover, low GLUL expression was associated with poor survival in breast cancer patients and with high HIF-1α-expressing patient subgroups. Our data reveal time-resolved changes in the regulation of metabolic proteins under oxygen-deprived conditions and elucidate GLUL as a strong responder to HIFs and the hypoxic environment. | ||
| 700 | 1 | |a Müller-Decker, Karin |e VerfasserIn |0 (DE-588)1084651351 |0 (DE-627)848630556 |0 (DE-576)169302431 |4 aut | |
| 700 | 1 | |a Wiemann, Stefan |e VerfasserIn |0 (DE-588)1105581209 |0 (DE-627)862699134 |0 (DE-576)473627191 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of proteome research |d Washington, DC : ACS Publications, 2002 |g 18(2019), 3, Seite 1352-1362 |h Online-Ressource |w (DE-627)340077174 |w (DE-600)2065254-9 |w (DE-576)096704608 |x 1535-3907 |7 nnas |a Functional proteomics of breast cancer metabolism identifies GLUL as responder during hypoxic adaptation |
| 773 | 1 | 8 | |g volume:18 |g year:2019 |g number:3 |g pages:1352-1362 |g extent:11 |a Functional proteomics of breast cancer metabolism identifies GLUL as responder during hypoxic adaptation |
| 856 | 4 | 0 | |u https://doi.org/10.1021/acs.jproteome.8b00944 |x Verlag |x Resolving-System |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20190514 | ||
| 993 | |a Article | ||
| 994 | |a 2019 | ||
| 998 | |g 1105581209 |a Wiemann, Stefan |m 1105581209:Wiemann, Stefan |d 140000 |e 140000PW1105581209 |k 0/140000/ |p 9 |y j | ||
| 998 | |g 1084651351 |a Müller-Decker, Karin |m 1084651351:Müller-Decker, Karin |d 140000 |e 140000PM1084651351 |k 0/140000/ |p 5 | ||
| 999 | |a KXP-PPN1665339624 |e 3475654997 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"recId":"1665339624","name":{"displayForm":["Stephan Bernhardt, Christian Tönsing, Devina Mitra, Nese Erdem, Karin Müller-Decker, Ulrike Korf, Clemens Kreutz, Jens Timmer, and Stefan Wiemann"]},"origin":[{"dateIssuedDisp":"January 4, 2019","dateIssuedKey":"2019"}],"id":{"eki":["1665339624"],"doi":["10.1021/acs.jproteome.8b00944"]},"physDesc":[{"extent":"11 S."}],"language":["eng"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"title":[{"title_sort":"Functional proteomics of breast cancer metabolism identifies GLUL as responder during hypoxic adaptation","title":"Functional proteomics of breast cancer metabolism identifies GLUL as responder during hypoxic adaptation"}],"person":[{"display":"Bernhardt, Stephan","family":"Bernhardt","given":"Stephan","role":"aut"},{"display":"Müller-Decker, Karin","role":"aut","given":"Karin","family":"Müller-Decker"},{"given":"Stefan","role":"aut","family":"Wiemann","display":"Wiemann, Stefan"}],"relHost":[{"part":{"extent":"11","text":"18(2019), 3, Seite 1352-1362","pages":"1352-1362","volume":"18","year":"2019","issue":"3"},"origin":[{"publisherPlace":"Washington, DC","dateIssuedDisp":"2002-","dateIssuedKey":"2002","publisher":"ACS Publications"}],"id":{"eki":["340077174"],"issn":["1535-3907"],"zdb":["2065254-9"]},"disp":"Functional proteomics of breast cancer metabolism identifies GLUL as responder during hypoxic adaptationJournal of proteome research","recId":"340077174","name":{"displayForm":["American Chemical Society"]},"title":[{"title_sort":"Journal of proteome research","title":"Journal of proteome research"}],"physDesc":[{"extent":"Online-Ressource"}],"pubHistory":["2002 -"],"language":["eng"],"note":["Gesehen am 25.08.2020"],"type":{"media":"Online-Ressource","bibl":"periodical"}}],"note":["Gesehen am 14.05.2019"]} | ||
| SRT | |a BERNHARDTSFUNCTIONAL4201 | ||