Buchumschlag

The anatomical location shapes the immune infiltrate in tumors of same etiology and affects survival

Purpose: The tumor immune microenvironment determines clinical outcome. Whether the original tissue in which a primary tumor develops influences this microenvironment is not well understood. - Experimental Design: We applied high-dimensional single-cell mass cytometry [Cytometry by Time-Of-Flight (C...

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Hauptverfasser: Santegoets, Saskia J. A. M. (VerfasserIn) , Charoentong, Pornpimol (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 2019
In: Clinical cancer research
Year: 2018, Jahrgang: 25, Heft: 1, Pages: 240-252
ISSN:1557-3265
DOI:10.1158/1078-0432.CCR-18-1749
Online-Zugang:Verlag, Volltext: https://doi.org/10.1158/1078-0432.CCR-18-1749
Verlag, Volltext: http://clincancerres.aacrjournals.org/content/25/1/240
Volltext
Verfasserangaben:Saskia J. Santegoets, Vanessa J. van Ham, Ilina Ehsan, Pornpimol Charoentong, Chantal L. Duurland, Vincent van Unen, Thomas Höllt, Lilly-Ann van der Velden, Sylvia L. van Egmond, Kim E. Kortekaas, Peggy J. de Vos van Steenwijk, Mariëtte I.E. van Poelgeest, Marij J.P. Welters, and Sjoerd H. van der Burg
Beschreibung
Zusammenfassung:Purpose: The tumor immune microenvironment determines clinical outcome. Whether the original tissue in which a primary tumor develops influences this microenvironment is not well understood. - Experimental Design: We applied high-dimensional single-cell mass cytometry [Cytometry by Time-Of-Flight (CyTOF)] analysis and functional studies to analyze immune cell populations in human papillomavirus (HPV)-induced primary tumors of the cervix (cervical carcinoma) and oropharynx (oropharyngeal squamous cell carcinoma, OPSCC). - Results: Despite the same etiology of these tumors, the composition and functionality of their lymphocytic infiltrate substantially differed. Cervical carcinoma displayed a 3-fold lower CD4:CD8 ratio and contained more activated CD8+CD103+CD161+ effector T cells and less CD4+CD161+ effector memory T cells than OPSCC. CD161+ effector cells produced the highest cytokine levels among tumor-specific T cells. Differences in CD4+ T-cell infiltration between cervical carcinoma and OPSCC were reflected in the detection rate of intratumoral HPV-specific CD4+ T cells and in their impact on OPSCC and cervical carcinoma survival. The peripheral blood mononuclear cell composition of these patients, however, was similar. - Conclusions: The tissue of origin significantly affects the overall shape of the immune infiltrate in primary tumors.
Beschreibung:published first September 17, 2018
Gesehen am 17.05.2019
Beschreibung:Online Resource
ISSN:1557-3265
DOI:10.1158/1078-0432.CCR-18-1749

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