Transcutaneous assessment of renal function in conscious rodents
Glomerular filtration rate (GFR) is the gold standard to assess overall kidney function. However, traditional methods to evaluate GFR are cumbersome and time-consuming. In addition, serial blood or urine samples are required, with the associated stress for the experimental animals. A recent techniqu...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article (Journal) Video |
| Language: | English |
| Published: |
3/26/2016
|
| In: |
JoVE. Video journal
Year: 2016, Issue: 109 |
| ISSN: | 1940-087X |
| DOI: | 10.3791/53767 |
| Subjects: | |
| Online Access: | Verlag, Volltext: https://doi.org/10.3791/53767 Verlag, Volltext: https://www.jove.com/video/53767/transcutaneous-assessment-of-renal-function-in-conscious-rodents 
|
| Author Notes: | Zeneida Herrera Pérez, Stefanie Weinfurter, Norbert Gretz |
MARC
| LEADER | 00000cga a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1666211958 | ||
| 003 | DE-627 | ||
| 005 | 20220816160559.0 | ||
| 006 | m o | | | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 190523s2016 xx ||| | o ||eng c | ||
| 024 | 7 | |a 10.3791/53767 |2 doi | |
| 035 | |a (DE-627)1666211958 | ||
| 035 | |a (DE-599)KXP1666211958 | ||
| 035 | |a (OCoLC)1341225386 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Herrera Pérez, Zeneida |d 1987- |e VerfasserIn |0 (DE-588)1070668591 |0 (DE-627)861238737 |0 (DE-576)432189580 |4 aut | |
| 245 | 1 | 0 | |a Transcutaneous assessment of renal function in conscious rodents |c Zeneida Herrera Pérez, Stefanie Weinfurter, Norbert Gretz |
| 264 | 1 | |c 3/26/2016 | |
| 300 | |a 1 Online-Ressource (1Videodatei, 7:18 min) |b farbig | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 336 | |a zweidimensionales bewegtes Bild |b tdi |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 23.05.2019 | ||
| 500 | |a Enthält auch den Video-Artikel | ||
| 500 | |a Wissenschaftlicher Film. Deutschland. 2016 | ||
| 520 | |a Glomerular filtration rate (GFR) is the gold standard to assess overall kidney function. However, traditional methods to evaluate GFR are cumbersome and time-consuming. In addition, serial blood or urine samples are required, with the associated stress for the experimental animals. A recent technique significantly reduces the investment in time and resources, minimizing the invasiveness and the animal stress, but being equally valid as the traditional approaches. The method measures transcutaneously renal function. Using an optical device and the exogenous renal marker fluorescein isothiocyanate (FITC)-sinistrin, this technique is capable of measuring the elimination kinetics of the marker through the skin. With neither blood nor urine samples nor the associated laboratory assays needed, the results of the transcutaneous measurement are almost instantaneously available. The method has been already validated in different species and successfully applied in several models of renal pathology. Moreover, due to its minimally invasive characteristics, it is suitable for sequential measurements within the same animal. Here is provided a detailed protocol to carry out the transcutaneous assessment of renal function in rodents. | ||
| 655 | 7 | |a Film |0 (DE-588)4017102-4 |0 (DE-627)104559683 |0 (DE-576)208918531 |2 gnd-content | |
| 700 | 1 | |a Weinfurter, Stefanie |d 1985- |e VerfasserIn |0 (DE-588)1129391728 |0 (DE-627)883961628 |0 (DE-576)486387933 |4 aut | |
| 700 | 1 | |a Gretz, Norbert |d 1954-2023 |e VerfasserIn |0 (DE-588)1020104589 |0 (DE-627)691105154 |0 (DE-576)359544843 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t JoVE. Video journal |d [Erscheinungsort nicht ermittelbar] : [Verlag nicht ermittelbar], 2006 |g (2016,109) Artikel-Nummer e53767, 8 Seiten |h Online-Ressource |w (DE-627)521454778 |w (DE-600)2259946-0 |w (DE-576)338522980 |x 1940-087X |7 nngs |a Transcutaneous assessment of renal function in conscious rodents |
| 773 | 1 | 8 | |g year:2016 |g number:109 |a Transcutaneous assessment of renal function in conscious rodents |
| 856 | 4 | 0 | |u https://doi.org/10.3791/53767 |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u https://www.jove.com/video/53767/transcutaneous-assessment-of-renal-function-in-conscious-rodents |x Verlag |3 Volltext |
| 935 | |c vide | ||
| 937 | |a Wissenschaftlicher Film |b Deutschland |c 2016 | ||
| 951 | |a AR | ||
| 992 | |a 20190523 | ||
| 993 | |a Video | ||
| 994 | |a 2016 | ||
| 998 | |g 1020104589 |a Gretz, Norbert |m 1020104589:Gretz, Norbert |d 60000 |d 999811 |e 60000PG1020104589 |e 999811PG1020104589 |k 0/60000/ |k 1/60000/999811/ |p 3 |y j | ||
| 998 | |g 1129391728 |a Weinfurter, Stefanie |m 1129391728:Weinfurter, Stefanie |d 60000 |d 999811 |e 60000PW1129391728 |e 999811PW1129391728 |k 0/60000/ |k 1/60000/999811/ |p 2 | ||
| 998 | |g 1070668591 |a Herrera Pérez, Zeneida |m 1070668591:Herrera Pérez, Zeneida |d 60000 |d 999811 |e 60000PH1070668591 |e 999811PH1070668591 |k 0/60000/ |k 1/60000/999811/ |p 1 |x j | ||
| 999 | |a KXP-PPN1666211958 |e 3479059791 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"relHost":[{"title":[{"title":"JoVE","title_sort":"JoVE","partname":"Video journal"}],"pubHistory":["1.2006 -"],"titleAlt":[{"title":"JoVE journal"},{"title":"JoVE journal Biology"},{"title":"JoVE journal Medicine"},{"title":"JoVE journal Behavior"},{"title":"JoVE journal Biochemistry"},{"title":"JoVE journal Bioengineering"},{"title":"JoVE journal Cancer research"},{"title":"JoVE journal Chemistry"},{"title":"JoVE journal Developmental biology"},{"title":"JoVE journal Engineering"},{"title":"JoVE journal Environment"},{"title":"JoVE journal Genetics"},{"title":"JoVE journal Immunology and infection"},{"title":"JoVE journal Neuroscience"},{"title":"Journal of visualized experiments"}],"part":{"text":"(2016,109) Artikel-Nummer e53767, 8 Seiten","issue":"109","year":"2016"},"disp":"Transcutaneous assessment of renal function in conscious rodentsJoVE. Video journal","note":["Gesehen am 10.02.25","Inhaltliche Gliederung in: Behavior; Biochemistry; Bioengineering; Biology; Cancer research; Chemistry; Developmental biology; Engineering; Environment; Genetics; Immunology and infection; Medicine; Neuroscience"],"type":{"media":"Online-Ressource","bibl":"periodical"},"language":["eng"],"recId":"521454778","origin":[{"publisherPlace":"[Erscheinungsort nicht ermittelbar]","dateIssuedDisp":"2006-","publisher":"[Verlag nicht ermittelbar]","dateIssuedKey":"2006"}],"id":{"eki":["521454778"],"zdb":["2259946-0"],"issn":["1940-087X"]},"physDesc":[{"extent":"Online-Ressource"}]}],"physDesc":[{"noteIll":"farbig","extent":"1 Online-Ressource (1Videodatei, 7:18 min) S."}],"name":{"displayForm":["Zeneida Herrera Pérez, Stefanie Weinfurter, Norbert Gretz"]},"id":{"doi":["10.3791/53767"],"eki":["1666211958"]},"origin":[{"dateIssuedKey":"2016","dateIssuedDisp":"3/26/2016"}],"language":["eng"],"recId":"1666211958","type":{"bibl":"article-journal","media":"Online-Ressource"},"note":["Gesehen am 23.05.2019","Enthält auch den Video-Artikel","Wissenschaftlicher Film. Deutschland. 2016"],"person":[{"family":"Herrera Pérez","given":"Zeneida","roleDisplay":"VerfasserIn","display":"Herrera Pérez, Zeneida","role":"aut"},{"role":"aut","display":"Weinfurter, Stefanie","roleDisplay":"VerfasserIn","given":"Stefanie","family":"Weinfurter"},{"role":"aut","display":"Gretz, Norbert","roleDisplay":"VerfasserIn","given":"Norbert","family":"Gretz"}],"title":[{"title":"Transcutaneous assessment of renal function in conscious rodents","title_sort":"Transcutaneous assessment of renal function in conscious rodents"}]} | ||
| SRT | |a HERRERAPERTRANSCUTAN3262 | ||