Inhibitor-induced dimerization of an essential oxidoreductase from african trypanosomes
Trypanosomal and leishmanial infections claim tens of thousands of lives each year. The metabolism of these unicellular eukaryotic parasites differs from the human host and their enzymes thus constitute promising drug targets. Tryparedoxin (Tpx) from Trypanosoma brucei is the essential oxidoreductas...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
03 January 2019
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| In: |
Angewandte Chemie. International edition
Year: 2019, Volume: 58, Issue: 11, Pages: 3640-3644 |
| ISSN: | 1521-3773 |
| DOI: | 10.1002/anie.201810470 |
| Online Access: | Verlag, Volltext: https://doi.org/10.1002/anie.201810470 Verlag, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.201810470 
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| Author Notes: | Annika Wagner, Thien Anh Le, Martha Brennich, Philipp Klein, Nicole Bader, Erika Diehl, Daniel Paszek, A. Katharina Weickhmann, Natalie Dirdjaja, R. Luise Krauth‐Siegel, Bernd Engels, Till Opatz, Hermann Schindelin, and Ute A. Hellmich |
| Summary: | Trypanosomal and leishmanial infections claim tens of thousands of lives each year. The metabolism of these unicellular eukaryotic parasites differs from the human host and their enzymes thus constitute promising drug targets. Tryparedoxin (Tpx) from Trypanosoma brucei is the essential oxidoreductase in the parasite's hydroperoxide-clearance cascade. In vitro and in vivo functional assays show that a small, selective inhibitor efficiently inhibits Tpx. With X-ray crystallography, SAXS, analytical SEC, SEC-MALS, MD simulations, ITC, and NMR spectroscopy, we show how covalent binding of this monofunctional inhibitor leads to Tpx dimerization. Intra- and intermolecular inhibitor-inhibitor, protein-protein, and inhibitor-protein interactions stabilize the dimer. The behavior of this efficient antitrypanosomal molecule thus constitutes an exquisite example of chemically induced dimerization with a small, monovalent ligand that can be exploited for future drug design. |
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| Item Description: | Gesehen am 27.05.2019 |
| Physical Description: | Online Resource |
| ISSN: | 1521-3773 |
| DOI: | 10.1002/anie.201810470 |