Single-pot, solid-phase-enhanced sample preparation for proteomics experiments

This protocol describes a single-pot, solid-phase-enhanced sample-preparation (SP3) method for rapid, robust, and efficient processing of protein samples for proteomic analysis.

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Bibliographic Details
Main Authors: Hughes, Christopher (Author) , Müller, Torsten (Author) , Krijgsveld, Jeroen (Author)
Format: Article (Journal)
Language:English
Published: 2019
In: Nature protocols
Year: 2018, Volume: 14, Issue: 1, Pages: 68-85
ISSN:1750-2799
DOI:10.1038/s41596-018-0082-x
Online Access:Verlag, Volltext: https://doi.org/10.1038/s41596-018-0082-x
Verlag, Volltext: https://www.nature.com/articles/s41596-018-0082-x
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Author Notes:Christopher S. Hughes, Sophie Moggridge, Torsten Müller, Poul H. Sorensen, Gregg B. Morin & Jeroen Krijgsveld
Description
Summary:This protocol describes a single-pot, solid-phase-enhanced sample-preparation (SP3) method for rapid, robust, and efficient processing of protein samples for proteomic analysis.
Item Description: Published: 21 November 2018
Gesehen am 05.06.2019
Physical Description:Online Resource
ISSN:1750-2799
DOI:10.1038/s41596-018-0082-x