Bioinformatic and experimental fishing for artemisinin-interacting proteins from human nasopharyngeal cancer cells
Determining interacting cellular partners of drugs by chemical proteomic techniques is complex and tedious. Most approaches rely on activity-based probe profiling and compound-centric chemical proteomics. The anti-malarial artemisinin also exerts profound anti-cancer activity, but the mechanisms of...
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| Hauptverfasser: | , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
06 Feb 2012
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| In: |
Molecular BioSystems
Year: 2012, Jahrgang: 8, Heft: 4, Pages: 1311-1318 |
| ISSN: | 1742-2051 |
| DOI: | 10.1039/C2MB05437J |
| Online-Zugang: | Verlag, Volltext: https://doi.org/10.1039/C2MB05437J Verlag, Volltext: https://pubs.rsc.org/en/content/articlelanding/2012/mb/c2mb05437j 
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| Verfasserangaben: | T. Eichhorn, S. Schloissnig, B. Hahn, A. Wendler, Rolf Mertens, W.D. Lehmann, R.L. Krauth-Siegel and T. Efferth |
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| 520 | |a Determining interacting cellular partners of drugs by chemical proteomic techniques is complex and tedious. Most approaches rely on activity-based probe profiling and compound-centric chemical proteomics. The anti-malarial artemisinin also exerts profound anti-cancer activity, but the mechanisms of action are incompletely understood. In the present investigation, we present a novel approach to identify artemisinin-interacting target proteins. Our approach overcomes usual problems in traditional fishing procedures, because the drug was attached to a surface without further chemical modification. The proteins identified effect among others, cell cycle arrest, apoptosis, inhibition of angiogenesis, disruption of cell migration, and modulation of nuclear receptor responsiveness. Furthermore, a bioinformatic approach confirmed experimentally identified proteins and suggested a large number of other interacting proteins. Theoretically predicted interaction partners may serve as a starting point to complete the whole set of proteins binding artemisinin. | ||
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