Structural basis for the assembly and nucleic acid binding of the TREX-2 transcription-export complex
The conserved TREX-2 transcription-export complex integrates transcription and processing of many actively transcribed nascent mRNAs with the recruitment of export factors at nuclear pores and also contributes to transcriptional memory and genomic stability. We report the crystal structure of the Sa...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
19 February 2012
|
| In: |
Nature structural & molecular biology
Year: 2012, Volume: 19, Issue: 3, Pages: 328-336 |
| ISSN: | 1545-9985 |
| DOI: | 10.1038/nsmb.2235 |
| Online Access: | Verlag, Volltext: https://doi.org/10.1038/nsmb.2235 Verlag, Volltext: https://www.nature.com/articles/nsmb.2235 
|
| Author Notes: | Andrew M. Ellisdon, Lyudmila Dimitrova, Ed Hurt & Murray Stewart |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1668060949 | ||
| 003 | DE-627 | ||
| 005 | 20220816175743.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 190627s2012 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1038/nsmb.2235 |2 doi | |
| 035 | |a (DE-627)1668060949 | ||
| 035 | |a (DE-599)KXP1668060949 | ||
| 035 | |a (OCoLC)1341230319 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Ellisdon, Andrew M. |e VerfasserIn |0 (DE-588)1186097884 |0 (DE-627)1665456728 |4 aut | |
| 245 | 1 | 0 | |a Structural basis for the assembly and nucleic acid binding of the TREX-2 transcription-export complex |c Andrew M. Ellisdon, Lyudmila Dimitrova, Ed Hurt & Murray Stewart |
| 264 | 1 | |c 19 February 2012 | |
| 300 | |a 9 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 27.06.2019 | ||
| 520 | |a The conserved TREX-2 transcription-export complex integrates transcription and processing of many actively transcribed nascent mRNAs with the recruitment of export factors at nuclear pores and also contributes to transcriptional memory and genomic stability. We report the crystal structure of the Sac3-Thp1-Sem1 segment of Saccharomyces cerevisiae TREX-2 that interfaces with the gene expression machinery. Sac3-Thp1-Sem1 forms a previously uncharacterized PCI-domain complex characterized by the juxtaposition of Sac3 and Thp1 winged helix domains, forming a platform that mediates nucleic acid binding. Our structure-guided mutations support the idea that the Thp1-Sac3 interaction is an essential requirement for mRNA binding and for the coupling of transcription and processing to mRNP assembly and export. These results provide insight into how newly synthesized transcripts are efficiently transferred from TREX-2 to the principal mRNA export factor, and they reveal how Sem1 stabilizes PCI domain-containing proteins and promotes complex assembly. | ||
| 700 | 1 | |a Dimitrova-Paternoga, Lyudmila Nikolova |e VerfasserIn |0 (DE-588)1081379626 |0 (DE-627)846034697 |0 (DE-576)454353138 |4 aut | |
| 700 | 1 | |a Hurt, Ed |d 1955- |e VerfasserIn |0 (DE-588)11021952X |0 (DE-627)729291863 |0 (DE-576)373308574 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Nature structural & molecular biology |d London [u.a.] : Nature Publishing Group, 2004 |g 19(2012), 3, Seite 328-336 |h Online-Ressource |w (DE-627)37683126X |w (DE-600)2131437-8 |w (DE-576)109293967 |x 1545-9985 |7 nnas |a Structural basis for the assembly and nucleic acid binding of the TREX-2 transcription-export complex |
| 773 | 1 | 8 | |g volume:19 |g year:2012 |g number:3 |g pages:328-336 |g extent:9 |a Structural basis for the assembly and nucleic acid binding of the TREX-2 transcription-export complex |
| 856 | 4 | 0 | |u https://doi.org/10.1038/nsmb.2235 |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u https://www.nature.com/articles/nsmb.2235 |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20190627 | ||
| 993 | |a Article | ||
| 994 | |a 2012 | ||
| 998 | |g 11021952X |a Hurt, Ed |m 11021952X:Hurt, Ed |d 700000 |d 711000 |e 700000PH11021952X |e 711000PH11021952X |k 0/700000/ |k 1/700000/711000/ |p 3 | ||
| 998 | |g 1081379626 |a Dimitrova-Paternoga, Lyudmila Nikolova |m 1081379626:Dimitrova-Paternoga, Lyudmila Nikolova |d 700000 |d 711000 |e 700000PD1081379626 |e 711000PD1081379626 |k 0/700000/ |k 1/700000/711000/ |p 2 | ||
| 999 | |a KXP-PPN1668060949 |e 3490053125 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"physDesc":[{"extent":"9 S."}],"relHost":[{"type":{"bibl":"periodical","media":"Online-Ressource"},"note":["Gesehen am 01.03.05"],"disp":"Structural basis for the assembly and nucleic acid binding of the TREX-2 transcription-export complexNature structural & molecular biology","recId":"37683126X","language":["eng"],"pubHistory":["11.2004 -"],"part":{"issue":"3","pages":"328-336","year":"2012","extent":"9","volume":"19","text":"19(2012), 3, Seite 328-336"},"title":[{"title_sort":"Nature structural & molecular biology","title":"Nature structural & molecular biology"}],"physDesc":[{"extent":"Online-Ressource"}],"origin":[{"publisher":"Nature Publishing Group","dateIssuedKey":"2004","dateIssuedDisp":"2004-","publisherPlace":"London [u.a.]"}],"id":{"eki":["37683126X"],"zdb":["2131437-8"],"issn":["1545-9985"]}}],"name":{"displayForm":["Andrew M. Ellisdon, Lyudmila Dimitrova, Ed Hurt & Murray Stewart"]},"origin":[{"dateIssuedKey":"2012","dateIssuedDisp":"19 February 2012"}],"id":{"eki":["1668060949"],"doi":["10.1038/nsmb.2235"]},"type":{"bibl":"article-journal","media":"Online-Ressource"},"note":["Gesehen am 27.06.2019"],"language":["eng"],"recId":"1668060949","person":[{"roleDisplay":"VerfasserIn","display":"Ellisdon, Andrew M.","role":"aut","family":"Ellisdon","given":"Andrew M."},{"role":"aut","display":"Dimitrova-Paternoga, Lyudmila Nikolova","roleDisplay":"VerfasserIn","given":"Lyudmila Nikolova","family":"Dimitrova-Paternoga"},{"family":"Hurt","given":"Ed","display":"Hurt, Ed","roleDisplay":"VerfasserIn","role":"aut"}],"title":[{"title":"Structural basis for the assembly and nucleic acid binding of the TREX-2 transcription-export complex","title_sort":"Structural basis for the assembly and nucleic acid binding of the TREX-2 transcription-export complex"}]} | ||
| SRT | |a ELLISDONANSTRUCTURAL1920 | ||