Evaluation of immunophenotypic and molecular biomarkers for Sézary syndrome using standard operating procedures: a multicenter study of 59 patients
Differentiation between Sézary syndrome and erythrodermic inflammatory dermatoses can be challenging, and a number of studies have attempted to identify characteristic immunophenotypic changes and molecular biomarkers in Sézary cells that could be useful as additional diagnostic criteria. In this...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
29 April 2016
|
| In: |
The journal of investigative dermatology
Year: 2016, Volume: 136, Issue: 7, Pages: 1364-1372 |
| ISSN: | 1523-1747 |
| DOI: | 10.1016/j.jid.2016.01.038 |
| Online Access: | Verlag, Volltext: https://doi.org/10.1016/j.jid.2016.01.038 Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S0022202X1600573X 
|
| Author Notes: | Stephanie E. Boonk, Willem H. Zoutman, Anne Marie-Cardine, Leslie van der Fits, Jacoba J. Out-Luiting, Tracey J. Mitchell, Isabella Tosi, Stephen L. Morris, Blaithin Moriarty, Nina Booken, Moritz Felcht, Pietro Quaglino, Renata Ponti, Emanuela Barberio, Caroline Ram-Wolff, Kirsi Jäntti, Annamari Ranki, Maria Grazia Bernengo, Claus-Detlev Klemke, Armand Bensussan, Laurence Michel, Sean Whittaker, Martine Bagot, Cornelis P. Tensen, Rein Willemze and Maarten H. Vermeer |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1668487551 | ||
| 003 | DE-627 | ||
| 005 | 20240323101142.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 190703s2016 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.jid.2016.01.038 |2 doi | |
| 035 | |a (DE-627)1668487551 | ||
| 035 | |a (DE-599)KXP1668487551 | ||
| 035 | |a (OCoLC)1341231838 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Boonk, Stéphanie E. |e VerfasserIn |0 (DE-588)1155942434 |0 (DE-627)1018497765 |0 (DE-576)501945237 |4 aut | |
| 245 | 1 | 0 | |a Evaluation of immunophenotypic and molecular biomarkers for Sézary syndrome using standard operating procedures |b a multicenter study of 59 patients |c Stephanie E. Boonk, Willem H. Zoutman, Anne Marie-Cardine, Leslie van der Fits, Jacoba J. Out-Luiting, Tracey J. Mitchell, Isabella Tosi, Stephen L. Morris, Blaithin Moriarty, Nina Booken, Moritz Felcht, Pietro Quaglino, Renata Ponti, Emanuela Barberio, Caroline Ram-Wolff, Kirsi Jäntti, Annamari Ranki, Maria Grazia Bernengo, Claus-Detlev Klemke, Armand Bensussan, Laurence Michel, Sean Whittaker, Martine Bagot, Cornelis P. Tensen, Rein Willemze and Maarten H. Vermeer |
| 264 | 1 | |c 29 April 2016 | |
| 300 | |a 9 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 03.07.2019 | ||
| 520 | |a Differentiation between Sézary syndrome and erythrodermic inflammatory dermatoses can be challenging, and a number of studies have attempted to identify characteristic immunophenotypic changes and molecular biomarkers in Sézary cells that could be useful as additional diagnostic criteria. In this European multicenter study, the sensitivity and specificity of these immunophenotypic and recently proposed but unconfirmed molecular biomarkers in Sézary syndrome were investigated. Peripheral blood CD4+ T cells from 59 patients with Sézary syndrome and 19 patients with erythrodermic inflammatory dermatoses were analyzed for cell surface proteins by flow cytometry and for copy number alterations and differential gene expression using custom-made quantitative PCR plates. Experiments were performed in duplicate in two independent centers using standard operating procedures with almost identical results. Sézary cells showed MYC gain (40%) and MNT loss (66%); up-regulation of DNM3 (75%), TWIST1 (69%), EPHA4 (66%), and PLS3 (66%); and down-regulation of STAT4 (91%). Loss of CD26 (≥80% CD4+ T cells) and/or CD7 (≥40% CD4+ T cells) and combination of altered expression of STAT4, TWIST1, and DNM3 or PLS3 could distinguish, respectively, 83% and 98% of patients with Sézary syndrome from patients with erythrodermic inflammatory dermatoses with 100% specificity. These additional diagnostic panels will be useful adjuncts in the differential diagnosis of Sézary syndrome versus erythrodermic inflammatory dermatoses. | ||
| 700 | 1 | |a Booken, Nina |d 1974- |e VerfasserIn |0 (DE-588)1030557462 |0 (DE-627)735716862 |0 (DE-576)378415654 |4 aut | |
| 700 | 1 | |a Felcht, Moritz |d 1979- |e VerfasserIn |0 (DE-588)133643441 |0 (DE-627)55029001X |0 (DE-576)299992721 |4 aut | |
| 700 | 1 | |a Klemke, Claus-Detlev |d 1969- |e VerfasserIn |0 (DE-588)122909615 |0 (DE-627)082229074 |0 (DE-576)293473145 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t The journal of investigative dermatology |d Amsterdam : Elsevier, 1938 |g 136(2016), 7, Seite 1364-1372 |h Online-Ressource |w (DE-627)320457990 |w (DE-600)2006902-9 |w (DE-576)09114289X |x 1523-1747 |7 nnas |a Evaluation of immunophenotypic and molecular biomarkers for Sézary syndrome using standard operating procedures a multicenter study of 59 patients |
| 773 | 1 | 8 | |g volume:136 |g year:2016 |g number:7 |g pages:1364-1372 |g extent:9 |a Evaluation of immunophenotypic and molecular biomarkers for Sézary syndrome using standard operating procedures a multicenter study of 59 patients |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.jid.2016.01.038 |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u http://www.sciencedirect.com/science/article/pii/S0022202X1600573X |x Verlag |x Resolving-System |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20190703 | ||
| 993 | |a Article | ||
| 994 | |a 2016 | ||
| 998 | |g 122909615 |a Klemke, Claus-Detlev |m 122909615:Klemke, Claus-Detlev |d 60000 |e 60000PK122909615 |k 0/60000/ |p 19 | ||
| 998 | |g 133643441 |a Felcht, Moritz |m 133643441:Felcht, Moritz |d 60000 |d 61900 |e 60000PF133643441 |e 61900PF133643441 |k 0/60000/ |k 1/60000/61900/ |p 11 | ||
| 998 | |g 1030557462 |a Booken, Nina |m 1030557462:Booken, Nina |d 60000 |d 61900 |e 60000PB1030557462 |e 61900PB1030557462 |k 0/60000/ |k 1/60000/61900/ |p 10 | ||
| 999 | |a KXP-PPN1668487551 |e 349078930X | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"origin":[{"dateIssuedKey":"2016","dateIssuedDisp":"29 April 2016"}],"language":["eng"],"physDesc":[{"extent":"9 S."}],"name":{"displayForm":["Stephanie E. Boonk, Willem H. Zoutman, Anne Marie-Cardine, Leslie van der Fits, Jacoba J. Out-Luiting, Tracey J. Mitchell, Isabella Tosi, Stephen L. Morris, Blaithin Moriarty, Nina Booken, Moritz Felcht, Pietro Quaglino, Renata Ponti, Emanuela Barberio, Caroline Ram-Wolff, Kirsi Jäntti, Annamari Ranki, Maria Grazia Bernengo, Claus-Detlev Klemke, Armand Bensussan, Laurence Michel, Sean Whittaker, Martine Bagot, Cornelis P. Tensen, Rein Willemze and Maarten H. Vermeer"]},"title":[{"title_sort":"Evaluation of immunophenotypic and molecular biomarkers for Sézary syndrome using standard operating procedures","title":"Evaluation of immunophenotypic and molecular biomarkers for Sézary syndrome using standard operating procedures","subtitle":"a multicenter study of 59 patients"}],"recId":"1668487551","id":{"doi":["10.1016/j.jid.2016.01.038"],"eki":["1668487551"]},"relHost":[{"pubHistory":["1.1938 -"],"type":{"bibl":"periodical","media":"Online-Ressource"},"note":["Gesehen am 16.02.16"],"physDesc":[{"extent":"Online-Ressource"}],"titleAlt":[{"title":"an international journal for research in cutaneous biology"}],"part":{"text":"136(2016), 7, Seite 1364-1372","volume":"136","year":"2016","issue":"7","pages":"1364-1372","extent":"9"},"recId":"320457990","disp":"Evaluation of immunophenotypic and molecular biomarkers for Sézary syndrome using standard operating procedures a multicenter study of 59 patientsThe journal of investigative dermatology","title":[{"title_sort":"journal of investigative dermatology","title":"The journal of investigative dermatology"}],"name":{"displayForm":["Society for Investigative Dermatology ; European Society for Dermatological Research"]},"corporate":[{"role":"isb","roleDisplay":"Herausgebendes Organ","display":"Society for Investigative Dermatology"}],"id":{"issn":["1523-1747"],"eki":["320457990"],"zdb":["2006902-9"]},"origin":[{"publisher":"Elsevier ; Blackwell ; Wiley-Blackwell ; Nature Publishing Group","publisherPlace":"Amsterdam ; Oxford [u.a.] ; [Erscheinungsort nicht ermittelbar] ; Basingstoke [u.a.]","dateIssuedKey":"1938","dateIssuedDisp":"1938-"}],"language":["eng"]}],"note":["Gesehen am 03.07.2019"],"person":[{"display":"Boonk, Stéphanie E.","given":"Stéphanie E.","role":"aut","family":"Boonk","roleDisplay":"VerfasserIn"},{"display":"Booken, Nina","given":"Nina","role":"aut","family":"Booken","roleDisplay":"VerfasserIn"},{"given":"Moritz","display":"Felcht, Moritz","family":"Felcht","roleDisplay":"VerfasserIn","role":"aut"},{"roleDisplay":"VerfasserIn","family":"Klemke","role":"aut","given":"Claus-Detlev","display":"Klemke, Claus-Detlev"}],"type":{"media":"Online-Ressource","bibl":"article-journal"}} | ||
| SRT | |a BOONKSTEPHEVALUATION2920 | ||