Assaying the contribution of membrane tension to clathrin-mediated endocytosis
Nowadays, live fluorescent microscopes allow us to study the dynamics of cellular processes in living cells with high spatial and temporal resolution. Since the implementation of this methodology to the field of clathrin-mediated endocytosis (CME), this approach has revolutionized our molecular unde...
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Dokumenttyp: | Kapitel/Artikel |
| Sprache: | Englisch |
| Veröffentlicht: |
21 August 2018
|
| In: |
Clathrin-mediated endoytosis
Year: 2018, Pages: 37-50 |
| Online-Zugang: | Verlag, Volltext: https://link.springer.com/protocol/10.1007%2F978-1-4939-8719-1_4
|
| Verfasserangaben: | Steeve Boulant |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1668619288 | ||
| 003 | DE-627 | ||
| 005 | 20220816183726.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 190704s2018 xx |||||o 00| ||eng c | ||
| 035 | |a (DE-627)1668619288 | ||
| 035 | |a (DE-599)KXP1668619288 | ||
| 035 | |a (OCoLC)1341232165 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Boulant, Steeve |e VerfasserIn |0 (DE-588)1130687430 |0 (DE-627)884888509 |0 (DE-576)48692243X |4 aut | |
| 245 | 1 | 0 | |a Assaying the contribution of membrane tension to clathrin-mediated endocytosis |c Steeve Boulant |
| 264 | 1 | |c 21 August 2018 | |
| 300 | |a 14 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 04.07.2019 | ||
| 520 | |a Nowadays, live fluorescent microscopes allow us to study the dynamics of cellular processes in living cells with high spatial and temporal resolution. Since the implementation of this methodology to the field of clathrin-mediated endocytosis (CME), this approach has revolutionized our molecular understanding of clathrin-driven cellular uptake. Conventional live cell microscopy approaches are used to determine the precise functions of specific proteins or lipids in orchestrating CME. Here, we will describe, in depth, the procedure to investigate the contribution of membrane tension in regulating clathrin-dependent endocytosis. We will explain two alternative methods to manipulate membrane tension while performing live fluorescence microscopy: cellular swelling through osmotic shock and cellular stretching of cells grown on stretchable silicon inserts. | ||
| 650 | 4 | |a Cellular stretching | |
| 650 | 4 | |a Clathrin-mediated endocytosis | |
| 650 | 4 | |a CME | |
| 650 | 4 | |a Live confocal microscopy | |
| 650 | 4 | |a Membrane tension | |
| 650 | 4 | |a Osmotic shock | |
| 773 | 0 | 8 | |i Enthalten in |t Clathrin-mediated endoytosis |d New York, NY : Humana Press, 2018 |g (2018), Seite 37-50 |h 1 Online-Ressource (xi, 253 pages) |w (DE-627)1030774803 |z 9781493987191 |z 1493987194 |7 nnam |a Assaying the contribution of membrane tension to clathrin-mediated endocytosis |
| 773 | 1 | 8 | |g year:2018 |g pages:37-50 |g extent:14 |a Assaying the contribution of membrane tension to clathrin-mediated endocytosis |
| 856 | 4 | 0 | |u https://link.springer.com/protocol/10.1007%2F978-1-4939-8719-1_4 |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20190704 | ||
| 993 | |a BookComponentPart | ||
| 994 | |a 2018 | ||
| 998 | |g 1130687430 |a Boulant, Steeve |m 1130687430:Boulant, Steeve |d 910000 |d 911700 |e 910000PB1130687430 |e 911700PB1130687430 |k 0/910000/ |k 1/910000/911700/ |p 1 |x j |y j | ||
| 999 | |a KXP-PPN1668619288 |e 3490926439 | ||
| BIB | |a Y | ||
| JSO | |a {"note":["Gesehen am 04.07.2019"],"relHost":[{"title":[{"subtitle":"methods and protocols","title":"Clathrin-mediated endoytosis","title_sort":"Clathrin-mediated endoytosis"}],"part":{"text":"(2018), Seite 37-50","year":"2018","extent":"14","pages":"37-50"},"language":["eng"],"type":{"media":"Online-Ressource","bibl":"edited-book"},"origin":[{"publisherPlace":"New York, NY","publisher":"Humana Press","dateIssuedDisp":"2018","dateIssuedKey":"2018"}],"recId":"1030774803","disp":"Assaying the contribution of membrane tension to clathrin-mediated endocytosisClathrin-mediated endoytosis","id":{"eki":["1030774803"],"doi":["10.1007/978-1-4939-8719-1"],"isbn":["9781493987191","1493987194","9781493987177","1493987178"]},"physDesc":[{"noteIll":"illustrations (some color)","extent":"1 Online-Ressource (xi, 253 pages)"}],"note":["Includes bibliographical references and index","Includes bibliographical references and index"],"name":{"displayForm":["edited by Laura E. Swan"]},"person":[{"given":"Laura","role":"edt","display":"Swan, Laura","family":"Swan"}]}],"physDesc":[{"extent":"14 S."}],"id":{"eki":["1668619288"]},"origin":[{"dateIssuedDisp":"21 August 2018","dateIssuedKey":"2018"}],"recId":"1668619288","type":{"bibl":"chapter","media":"Online-Ressource"},"person":[{"given":"Steeve","role":"aut","display":"Boulant, Steeve","family":"Boulant"}],"name":{"displayForm":["Steeve Boulant"]},"language":["eng"],"title":[{"title_sort":"Assaying the contribution of membrane tension to clathrin-mediated endocytosis","title":"Assaying the contribution of membrane tension to clathrin-mediated endocytosis"}]} | ||
| SRT | |a BOULANTSTEASSAYINGTH2120 | ||