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The ESCRT complexes: methods and protocols

Analysis of the Archaeal ESCRT Apparatus -- Genetic and Cell Biology Methods to Study ESCRTs in Drosophila melanogaster -- Functional Analysis of ESCRT-Positive Extracellular Vesicles in the Drosophila Wing Imaginal Disc -- Subcellular Localization of ESCRT-II in the Nematode C. elegans by Correlati...

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Bibliographic Details
Other Authors: Culetto, Emmanuel (Editor) , Legouis, Renaud (Editor)
Format: Book/Monograph
Language:English
Published: New York, NY Humana 2019
Edition:1st ed. 2019
Series:Methods in molecular biology 1998
In: Methods in molecular biology (1998)

Volumes / Articles: Show Volumes / Articles.
DOI:10.1007/978-1-4939-9492-2
Online Access:Resolving-System: https://doi.org/10.1007/978-1-4939-9492-2
Resolving-System, lizenzpflichtig: https://doi.org/10.1007/978-1-4939-9492-2
Cover: https://swbplus.bsz-bw.de/bsz1668655756cov.jpg
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Author Notes:edited by Emmanuel Culetto, Renaud Legouis

MARC

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520 |a This detailed collection gathers both established and recent technical procedures to study the Endosomal Sorting Complex Required for Transport (ESCRT) complexes in a wide range of biological systems: Archaea, A. thaliana, U. maydis, S. cerevisiae, S. pombe, C. elegans, D. melanogaster, and mammalian cells. Opening with a section on imaging techniques, the book continues with chapters covering biochemical approaches presenting strategies for production and characterization of recombinant ESCRT proteins, or of specific ESCRT protein domains, as well as genetic and proteomic experimental approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, The ESCRT Complexes: Methods and Protocols serves as a compact guide for researchers interested in establishing an integrated approach to investigate the ESCRT machinery functions in cell biology 
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