Identification of MALDI imaging proteolytic peptides using LC-MS/MS-based biomarker discovery data: a proof of concept
Purpose Identification of proteolytic peptides from matrix-assisted laser desorption/ionization (MALDI) imaging remains a challenge. The low fragmentation yields obtained using in situ post source decay impairs identification. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is an alternati...
Gespeichert in:
| Hauptverfasser: | , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
January 2019
|
| In: |
Proteomics. Clinical applications
Year: 2018, Jahrgang: 13, Heft: 1 |
| ISSN: | 1862-8354 |
| DOI: | 10.1002/prca.201800158 |
| Online-Zugang: | Verlag, Volltext: https://doi.org/10.1002/prca.201800158 Verlag, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/prca.201800158 
|
| Verfasserangaben: | Rémi Longuespée, Alice Ly, Rita Casadonte, Kristina Schwamborn, Daniel Kazdal, Christiane Zgorzelski, Christine Bollwein, Katharina Kriegsmann, Wilko Weichert, Jörg Kriegsmann, Peter Schirmacher, Margaux Fresnais, Cristiano Oliveira, Mark Kriegsmann |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1668719347 | ||
| 003 | DE-627 | ||
| 005 | 20220816184519.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 190708r20192018xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1002/prca.201800158 |2 doi | |
| 035 | |a (DE-627)1668719347 | ||
| 035 | |a (DE-599)KXP1668719347 | ||
| 035 | |a (OCoLC)1341233101 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Longuespée, Rémi |e VerfasserIn |0 (DE-588)113707146X |0 (DE-627)89404690X |0 (DE-576)491093179 |4 aut | |
| 245 | 1 | 0 | |a Identification of MALDI imaging proteolytic peptides using LC-MS/MS-based biomarker discovery data |b a proof of concept |c Rémi Longuespée, Alice Ly, Rita Casadonte, Kristina Schwamborn, Daniel Kazdal, Christiane Zgorzelski, Christine Bollwein, Katharina Kriegsmann, Wilko Weichert, Jörg Kriegsmann, Peter Schirmacher, Margaux Fresnais, Cristiano Oliveira, Mark Kriegsmann |
| 264 | 1 | |c January 2019 | |
| 300 | |b Illustrationen | ||
| 300 | |a 5 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a First published: 07 December 2018 | ||
| 500 | |a Gesehen am 08.07.2019 | ||
| 520 | |a Purpose Identification of proteolytic peptides from matrix-assisted laser desorption/ionization (MALDI) imaging remains a challenge. The low fragmentation yields obtained using in situ post source decay impairs identification. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is an alternative to in situ MS/MS, but leads to multiple identification candidates for a given mass. The authors propose to use LC-MS/MS-based biomarker discovery results to reliably identify proteolytic peptides from MALDI imaging. Experimental design The authors defined m/z values of interest for high grade squamous intraepithelial lesion (HSIL) by MALDI imaging. In parallel the authors used data from a biomarker discovery study to correlate m/z from MALDI imaging with masses of peptides identified by LC-MS/MS in HSIL. The authors neglected candidates that were not significantly more abundant in HSIL according to the biomarker discovery investigation. Results The authors assigned identifications to three m/z of interest. The number of possible identifiers for MALDI imaging m/z peaks using LC-MS/MS-based biomarker discovery studies was reduced by about tenfold compared using a single LC-MS/MS experiment. One peptide identification candidate was validated by immunohistochemistry. Conclusion and clinical relevance This concept combines LC-MS/MS-based quantitative proteomics with MALDI imaging and allows reliable peptide identification. Public datasets from LC-MS/MS biomarker discovery experiments will be useful to identify MALDI imaging m/z peaks. | ||
| 534 | |c 2018 | ||
| 650 | 4 | |a identification | |
| 650 | 4 | |a liquid chromatography tandem mass spectrometry | |
| 650 | 4 | |a matrix-assisted laser desorption/ionization imaging | |
| 650 | 4 | |a microproteomics | |
| 700 | 1 | |a Kazdal, Daniel |d 1983- |e VerfasserIn |0 (DE-588)114929650X |0 (DE-627)1009614088 |0 (DE-576)496635867 |4 aut | |
| 700 | 1 | |a Zgorzelski, Christiane |e VerfasserIn |0 (DE-588)1167972317 |0 (DE-627)1031669019 |0 (DE-576)511377622 |4 aut | |
| 700 | 1 | |a Kriegsmann, Katharina |d 1986- |e VerfasserIn |0 (DE-588)1049422449 |0 (DE-627)781924006 |0 (DE-576)40339774X |4 aut | |
| 700 | 1 | |a Schirmacher, Peter |d 1961- |e VerfasserIn |0 (DE-588)1020440112 |0 (DE-627)691221197 |0 (DE-576)360427448 |4 aut | |
| 700 | 1 | |a Fresnais, Margaux |e VerfasserIn |0 (DE-588)1190043157 |0 (DE-627)1668720108 |4 aut | |
| 700 | 1 | |a Oliveira, Cristiano |e VerfasserIn |0 (DE-588)1157020739 |0 (DE-627)1020012528 |0 (DE-576)50257268X |4 aut | |
| 700 | 1 | |a Kriegsmann, Mark |d 1987- |e VerfasserIn |0 (DE-588)103740324X |0 (DE-627)755668782 |0 (DE-576)39141870X |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Proteomics. Clinical applications |d Weinheim : Wiley VCH, 2007 |g 13(2019,1) Artikel-Nummer 1800158, Seite 1-5, 5 Seiten |h Online-Ressource |w (DE-627)530281872 |w (DE-600)2317130-3 |w (DE-576)277494516 |x 1862-8354 |7 nnas |a Identification of MALDI imaging proteolytic peptides using LC-MS/MS-based biomarker discovery data a proof of concept |
| 773 | 1 | 8 | |g volume:13 |g year:2019 |g number:1 |g extent:5 |a Identification of MALDI imaging proteolytic peptides using LC-MS/MS-based biomarker discovery data a proof of concept |
| 856 | 4 | 0 | |u https://doi.org/10.1002/prca.201800158 |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u https://onlinelibrary.wiley.com/doi/abs/10.1002/prca.201800158 |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20190708 | ||
| 993 | |a Article | ||
| 994 | |a 2019 | ||
| 998 | |g 103740324X |a Kriegsmann, Mark |m 103740324X:Kriegsmann, Mark |d 910000 |d 912000 |e 910000PK103740324X |e 912000PK103740324X |k 0/910000/ |k 1/910000/912000/ |p 14 |y j | ||
| 998 | |g 1157020739 |a Oliveira, Cristiano |m 1157020739:Oliveira, Cristiano |p 13 | ||
| 998 | |g 1190043157 |a Fresnais, Margaux |m 1190043157:Fresnais, Margaux |p 12 | ||
| 998 | |g 1020440112 |a Schirmacher, Peter |m 1020440112:Schirmacher, Peter |d 910000 |d 912000 |e 910000PS1020440112 |e 912000PS1020440112 |k 0/910000/ |k 1/910000/912000/ |p 11 | ||
| 998 | |g 1049422449 |a Kriegsmann, Katharina |m 1049422449:Kriegsmann, Katharina |d 910000 |d 910100 |e 910000PK1049422449 |e 910100PK1049422449 |k 0/910000/ |k 1/910000/910100/ |p 8 | ||
| 998 | |g 1167972317 |a Zgorzelski, Christiane |m 1167972317:Zgorzelski, Christiane |d 910000 |d 912000 |e 910000PZ1167972317 |e 912000PZ1167972317 |k 0/910000/ |k 1/910000/912000/ |p 6 | ||
| 998 | |g 114929650X |a Kazdal, Daniel |m 114929650X:Kazdal, Daniel |d 910000 |d 912000 |e 910000PK114929650X |e 912000PK114929650X |k 0/910000/ |k 1/910000/912000/ |p 5 | ||
| 998 | |g 113707146X |a Longuespée, Rémi |m 113707146X:Longuespée, Rémi |d 910000 |d 912000 |e 910000PL113707146X |e 912000PL113707146X |k 0/910000/ |k 1/910000/912000/ |p 1 |x j | ||
| 999 | |a KXP-PPN1668719347 |e 3491815169 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"title":[{"subtitle":"a proof of concept","title":"Identification of MALDI imaging proteolytic peptides using LC-MS/MS-based biomarker discovery data","title_sort":"Identification of MALDI imaging proteolytic peptides using LC-MS/MS-based biomarker discovery data"}],"person":[{"display":"Longuespée, Rémi","family":"Longuespée","given":"Rémi","role":"aut"},{"display":"Kazdal, Daniel","given":"Daniel","role":"aut","family":"Kazdal"},{"role":"aut","given":"Christiane","family":"Zgorzelski","display":"Zgorzelski, Christiane"},{"display":"Kriegsmann, Katharina","family":"Kriegsmann","role":"aut","given":"Katharina"},{"role":"aut","given":"Peter","family":"Schirmacher","display":"Schirmacher, Peter"},{"role":"aut","given":"Margaux","family":"Fresnais","display":"Fresnais, Margaux"},{"display":"Oliveira, Cristiano","given":"Cristiano","role":"aut","family":"Oliveira"},{"given":"Mark","role":"aut","family":"Kriegsmann","display":"Kriegsmann, Mark"}],"type":{"media":"Online-Ressource","bibl":"article-journal"},"physDesc":[{"extent":"5 S.","noteIll":"Illustrationen"}],"language":["eng"],"origin":[{"dateIssuedKey":"2019","dateIssuedDisp":"January 2019"}],"id":{"doi":["10.1002/prca.201800158"],"eki":["1668719347"]},"recId":"1668719347","name":{"displayForm":["Rémi Longuespée, Alice Ly, Rita Casadonte, Kristina Schwamborn, Daniel Kazdal, Christiane Zgorzelski, Christine Bollwein, Katharina Kriegsmann, Wilko Weichert, Jörg Kriegsmann, Peter Schirmacher, Margaux Fresnais, Cristiano Oliveira, Mark Kriegsmann"]},"note":["First published: 07 December 2018","Gesehen am 08.07.2019"],"relHost":[{"pubHistory":["1.2007 -"],"language":["eng"],"physDesc":[{"extent":"Online-Ressource"}],"titleAlt":[{"title":"Proteomics / Clinical applications"},{"title":"Proteomics clinical applications"}],"note":["Fortsetzung der Druckausgabe","Gesehen am 5. November 2022"],"type":{"bibl":"periodical","media":"Online-Ressource"},"title":[{"partname":"Clinical applications","title_sort":"Proteomics","title":"Proteomics"}],"disp":"Identification of MALDI imaging proteolytic peptides using LC-MS/MS-based biomarker discovery data a proof of conceptProteomics. Clinical applications","recId":"530281872","part":{"year":"2019","issue":"1","extent":"5","text":"13(2019,1) Artikel-Nummer 1800158, Seite 1-5, 5 Seiten","volume":"13"},"origin":[{"dateIssuedDisp":"2007-","dateIssuedKey":"2007","publisher":"Wiley VCH","publisherPlace":"Weinheim"}],"id":{"issn":["1862-8354"],"zdb":["2317130-3"],"eki":["530281872"],"doi":["10.1002/(ISSN)1862-8354"]}}]} | ||
| SRT | |a LONGUESPEEIDENTIFICA2019 | ||