Knock-down of oncohistone H3F3A-G34W counteracts the neoplastic phenotype of giant cell tumor of bone derived stromal cells
Giant cell tumors of bone (GCTB) are semi-malignant tumors associated with extensive osteolytic defects and massive bone destructions. They display a locally aggressive behavior and a very high recurrence rate. Recently, a single mutation has been identified in GCTB affecting the H3F3A gene coding f...
Gespeichert in:
| Hauptverfasser: | , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
8 February 2019
|
| In: |
Cancer letters
Year: 2019, Jahrgang: 448, Pages: 61-69 |
| ISSN: | 1872-7980 |
| DOI: | 10.1016/j.canlet.2019.02.001 |
| Online-Zugang: | Verlag, Volltext: https://doi.org/10.1016/j.canlet.2019.02.001 Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S0304383519300631 
|
| Verfasserangaben: | J. Fellenberg, H. Sähr, D. Mancarella, C. Plass, A.M. Lindroth, F. Westhauser, B. Lehner, V. Ewerbeck |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1668836467 | ||
| 003 | DE-627 | ||
| 005 | 20220816185821.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 190709s2019 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.canlet.2019.02.001 |2 doi | |
| 035 | |a (DE-627)1668836467 | ||
| 035 | |a (DE-599)KXP1668836467 | ||
| 035 | |a (OCoLC)1341233383 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Fellenberg, Jörg |e VerfasserIn |0 (DE-588)1063323932 |0 (DE-627)812309219 |0 (DE-576)171796543 |4 aut | |
| 245 | 1 | 0 | |a Knock-down of oncohistone H3F3A-G34W counteracts the neoplastic phenotype of giant cell tumor of bone derived stromal cells |c J. Fellenberg, H. Sähr, D. Mancarella, C. Plass, A.M. Lindroth, F. Westhauser, B. Lehner, V. Ewerbeck |
| 264 | 1 | |c 8 February 2019 | |
| 300 | |a 9 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 09.07.2019 | ||
| 520 | |a Giant cell tumors of bone (GCTB) are semi-malignant tumors associated with extensive osteolytic defects and massive bone destructions. They display a locally aggressive behavior and a very high recurrence rate. Recently, a single mutation has been identified in GCTB affecting the H3F3A gene coding for the histone variant H3.3 (H3.3-G34W). The aim of this study was to investigate whether H3.3-G34W is sufficient to drive tumorigenesis in GCTB. Initially, we confirmed the high frequency of this mutation in 94% of 84 analyzed tissue samples. Using a siRNA based approach we could selectively knockdown H3.3-G34W in primary neoplastic stromal cells isolated from tumor tissue (GCTSC). H3.3-G34W knockdown caused a significant inhibition of cell proliferation, migration and colony formation capacity in vitro. Xenotransplantation of GCTSCs onto the chorioallantoic membrane of fertilized chicken eggs further demonstrated a significant impact of H3.3-G34W knockdown on tumor engraftment and growth in vivo. Our data indicate that H3.3-G34W is sufficient to drive tumorigenesis in GCTB. Apart from the application of H3.3-G34W screening as diagnostic tool, our data suggest that H3.3-G4W represents a promising target for the development of new GCTB therapies. | ||
| 650 | 4 | |a CAM assay | |
| 650 | 4 | |a Giant cell tumor of bone | |
| 650 | 4 | |a H3.3-G34W | |
| 650 | 4 | |a Histone | |
| 650 | 4 | |a Neoplastic transformation | |
| 700 | 1 | |a Sähr, Heiner |e VerfasserIn |0 (DE-588)1063323940 |0 (DE-627)812309316 |0 (DE-576)42184650X |4 aut | |
| 700 | 1 | |a Mancarella, D. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Plass, C. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Lindroth, A. M. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Westhauser, Fabian |d 1990- |e VerfasserIn |0 (DE-588)1121326501 |0 (DE-627)874251060 |0 (DE-576)480821496 |4 aut | |
| 700 | 1 | |a Lehner, Burkhard |e VerfasserIn |0 (DE-588)1063147808 |0 (DE-627)810647311 |0 (DE-576)420525297 |4 aut | |
| 700 | 1 | |a Ewerbeck, Volker |d 1950- |e VerfasserIn |0 (DE-588)172066190 |0 (DE-627)696972069 |0 (DE-576)132940264 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Cancer letters |d Amsterdam [u.a.] : Elsevier Science, 1975 |g 448(2019), Seite 61-69 |h Online-Ressource |w (DE-627)320434796 |w (DE-600)2004212-7 |w (DE-576)261677926 |x 1872-7980 |7 nnas |a Knock-down of oncohistone H3F3A-G34W counteracts the neoplastic phenotype of giant cell tumor of bone derived stromal cells |
| 773 | 1 | 8 | |g volume:448 |g year:2019 |g pages:61-69 |g extent:9 |a Knock-down of oncohistone H3F3A-G34W counteracts the neoplastic phenotype of giant cell tumor of bone derived stromal cells |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.canlet.2019.02.001 |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u http://www.sciencedirect.com/science/article/pii/S0304383519300631 |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20190709 | ||
| 993 | |a Article | ||
| 994 | |a 2019 | ||
| 998 | |g 172066190 |a Ewerbeck, Volker |m 172066190:Ewerbeck, Volker |d 910000 |d 911460 |e 910000PE172066190 |e 911460PE172066190 |k 0/910000/ |k 1/910000/911460/ |p 8 |y j | ||
| 998 | |g 1063147808 |a Lehner, Burkhard |m 1063147808:Lehner, Burkhard |d 910000 |d 911460 |e 910000PL1063147808 |e 911460PL1063147808 |k 0/910000/ |k 1/910000/911460/ |p 7 | ||
| 998 | |g 1121326501 |a Westhauser, Fabian |m 1121326501:Westhauser, Fabian |d 910000 |d 911460 |e 910000PW1121326501 |e 911460PW1121326501 |k 0/910000/ |k 1/910000/911460/ |p 6 | ||
| 998 | |g 1063323940 |a Sähr, Heiner |m 1063323940:Sähr, Heiner |d 50000 |e 50000PS1063323940 |k 0/50000/ |p 2 | ||
| 998 | |g 1063323932 |a Fellenberg, Jörg |m 1063323932:Fellenberg, Jörg |d 910000 |d 911460 |e 910000PF1063323932 |e 911460PF1063323932 |k 0/910000/ |k 1/910000/911460/ |p 1 |x j | ||
| 999 | |a KXP-PPN1668836467 |e 3492221645 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"note":["Gesehen am 09.07.2019"],"origin":[{"dateIssuedDisp":"8 February 2019","dateIssuedKey":"2019"}],"relHost":[{"language":["eng"],"id":{"zdb":["2004212-7"],"eki":["320434796"],"issn":["1872-7980"]},"origin":[{"publisherPlace":"Amsterdam [u.a.]","publisher":"Elsevier Science","dateIssuedDisp":"1975-","dateIssuedKey":"1975"}],"note":["Gesehen am 22.01.2020"],"pubHistory":["1.1975 -"],"recId":"320434796","type":{"media":"Online-Ressource","bibl":"periodical"},"title":[{"subtitle":"an international journal providing a forum for original and pertinent contributions in cancer research","title_sort":"Cancer letters","title":"Cancer letters"}],"part":{"year":"2019","pages":"61-69","text":"448(2019), Seite 61-69","volume":"448","extent":"9"},"physDesc":[{"extent":"Online-Ressource"}],"disp":"Knock-down of oncohistone H3F3A-G34W counteracts the neoplastic phenotype of giant cell tumor of bone derived stromal cellsCancer letters"}],"recId":"1668836467","type":{"bibl":"article-journal","media":"Online-Ressource"},"name":{"displayForm":["J. Fellenberg, H. Sähr, D. Mancarella, C. Plass, A.M. Lindroth, F. Westhauser, B. Lehner, V. Ewerbeck"]},"language":["eng"],"id":{"doi":["10.1016/j.canlet.2019.02.001"],"eki":["1668836467"]},"physDesc":[{"extent":"9 S."}],"title":[{"title":"Knock-down of oncohistone H3F3A-G34W counteracts the neoplastic phenotype of giant cell tumor of bone derived stromal cells","title_sort":"Knock-down of oncohistone H3F3A-G34W counteracts the neoplastic phenotype of giant cell tumor of bone derived stromal cells"}],"person":[{"role":"aut","given":"Jörg","display":"Fellenberg, Jörg","family":"Fellenberg"},{"display":"Sähr, Heiner","given":"Heiner","role":"aut","family":"Sähr"},{"role":"aut","given":"D.","display":"Mancarella, D.","family":"Mancarella"},{"given":"C.","display":"Plass, C.","role":"aut","family":"Plass"},{"family":"Lindroth","role":"aut","given":"A. M.","display":"Lindroth, A. M."},{"role":"aut","given":"Fabian","display":"Westhauser, Fabian","family":"Westhauser"},{"family":"Lehner","role":"aut","display":"Lehner, Burkhard","given":"Burkhard"},{"family":"Ewerbeck","given":"Volker","display":"Ewerbeck, Volker","role":"aut"}]} | ||
| SRT | |a FELLENBERGKNOCKDOWNO8201 | ||