Human glioblastoma stem-like cells accumulate protoporphyrin IX when subjected to exogenous 5-aminolaevulinic acid, rendering them sensitive to photodynamic treatment
Glioblastoma (GBM) is the most frequent and lethal primary brain tumor in adults. Despite multimodal therapy combining resection, radio- and alkylating chemotherapy, disease recurrence is universal and prognosis of patients is poor. Glioblastoma stem-like cells (GSC), which can be grown as neurosphe...
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
October 2016
|
| In: |
Journal of photochemistry and photobiology. B, Biology
Year: 2016, Jahrgang: 163, Pages: 203-210 |
| ISSN: | 1873-2682 |
| DOI: | 10.1016/j.jphotobiol.2016.08.043 |
| Online-Zugang: | Verlag, Volltext: https://doi.org/10.1016/j.jphotobiol.2016.08.043 Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S1011134416305322 
|
| Verfasserangaben: | Adrian Schimanski, Lara Ebbert, Michael C. Sabel, Gaetano Finocchiaro, Katrin Lamszus, Christian Ewelt, Nima Etminan, Johannes C. Fischer, Rüdiger V. Sorg |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1669336328 | ||
| 003 | DE-627 | ||
| 005 | 20230428192305.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 190717s2016 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.jphotobiol.2016.08.043 |2 doi | |
| 035 | |a (DE-627)1669336328 | ||
| 035 | |a (DE-599)KXP1669336328 | ||
| 035 | |a (OCoLC)1341233686 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Schimanski, Adrian |e VerfasserIn |0 (DE-588)1156542367 |0 (DE-627)1019340304 |0 (DE-576)502237511 |4 aut | |
| 245 | 1 | 0 | |a Human glioblastoma stem-like cells accumulate protoporphyrin IX when subjected to exogenous 5-aminolaevulinic acid, rendering them sensitive to photodynamic treatment |c Adrian Schimanski, Lara Ebbert, Michael C. Sabel, Gaetano Finocchiaro, Katrin Lamszus, Christian Ewelt, Nima Etminan, Johannes C. Fischer, Rüdiger V. Sorg |
| 264 | 1 | |c October 2016 | |
| 300 | |a 8 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 17.07.2019 | ||
| 500 | |a Available online 27 August 2016 | ||
| 520 | |a Glioblastoma (GBM) is the most frequent and lethal primary brain tumor in adults. Despite multimodal therapy combining resection, radio- and alkylating chemotherapy, disease recurrence is universal and prognosis of patients is poor. Glioblastoma stem-like cells (GSC), which can be grown as neurospheres from primary tumors in vitro, appear to be resistant to the established therapies and are suspected to be the driving force for disease recurrence. Thus, efficacy of emerging therapies may depend on targeting GSC. 5-aminolaevulinic acid-mediated photodynamic therapy (5-ALA/PDT) is a promising therapeutic approach in GBM. It utilizes the selective accumulation of the photosensitizer protoporphyrin IX (PPIX) in GBM cells after application of 5-ALA. When exposed to laser light of 635nm wavelength, PPIX initiates a photochemical reaction resulting in the generation of reactive oxygen species, which kill the tumor cells. Whether GSC accumulate PPIX and are sensitive to 5-ALA/PDT is currently unknown. Therefore, human GSC were derived from primary tumors and grown as neurospheres under serum free conditions. When subjected to exogenous 5-ALA, a dose- and time-dependent accumulation of PPIX in GSC was observed by flow cytometry, which varied between individual GSC preparations. Subsequent exposure to laser light of 635nm wavelength substantially killed GSC, whereas treatment with 5-ALA or exposure to laser light only had no effect. LD50 values differed between GSC preparations, but were negatively correlated with PPIX accumulation in GSC. In summary, we report for the first time that glioblastoma stem-like cells accumulate PPIX when subjected to 5-aminolaevulinic acid and are sensitive to 5-aminolaevulinc acid based photodynamic therapy. | ||
| 650 | 4 | |a 5-aminolaevulinic acid | |
| 650 | 4 | |a Cancer stem cells | |
| 650 | 4 | |a Glioblastoma | |
| 650 | 4 | |a Glioblastoma stem-like cells | |
| 650 | 4 | |a Photodynamic therapy | |
| 650 | 4 | |a Protoporphyrin IX | |
| 700 | 1 | |a Etminan, Nima |d 1978- |e VerfasserIn |0 (DE-588)134245202 |0 (DE-627)56561374X |0 (DE-576)300399871 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of photochemistry and photobiology. B, Biology |d New York, NY [u.a.] : Elsevier, 1987 |g 163(2016), Seite 203-210 |h Online-Ressource |w (DE-627)300591519 |w (DE-600)1482691-4 |w (DE-576)098330195 |x 1873-2682 |7 nnas |a Human glioblastoma stem-like cells accumulate protoporphyrin IX when subjected to exogenous 5-aminolaevulinic acid, rendering them sensitive to photodynamic treatment |
| 773 | 1 | 8 | |g volume:163 |g year:2016 |g pages:203-210 |g extent:8 |a Human glioblastoma stem-like cells accumulate protoporphyrin IX when subjected to exogenous 5-aminolaevulinic acid, rendering them sensitive to photodynamic treatment |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.jphotobiol.2016.08.043 |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u http://www.sciencedirect.com/science/article/pii/S1011134416305322 |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20190717 | ||
| 993 | |a Article | ||
| 994 | |a 2016 | ||
| 998 | |g 134245202 |a Etminan, Nima |m 134245202:Etminan, Nima |d 60000 |d 62600 |e 60000PE134245202 |e 62600PE134245202 |k 0/60000/ |k 1/60000/62600/ |p 7 | ||
| 999 | |a KXP-PPN1669336328 |e 3493971532 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"id":{"doi":["10.1016/j.jphotobiol.2016.08.043"],"eki":["1669336328"]},"recId":"1669336328","type":{"media":"Online-Ressource","bibl":"article-journal"},"origin":[{"dateIssuedDisp":"October 2016","dateIssuedKey":"2016"}],"name":{"displayForm":["Adrian Schimanski, Lara Ebbert, Michael C. Sabel, Gaetano Finocchiaro, Katrin Lamszus, Christian Ewelt, Nima Etminan, Johannes C. Fischer, Rüdiger V. Sorg"]},"note":["Gesehen am 17.07.2019","Available online 27 August 2016"],"language":["eng"],"person":[{"display":"Schimanski, Adrian","role":"aut","family":"Schimanski","given":"Adrian"},{"display":"Etminan, Nima","role":"aut","family":"Etminan","given":"Nima"}],"relHost":[{"language":["eng"],"titleAlt":[{"title":"Journal of photochemistry and photobiology / B"}],"pubHistory":["1.1987/88 - 105.2011; Vol. 106.2012 -"],"corporate":[{"display":"European Society for Photobiology","role":"isb"}],"origin":[{"dateIssuedDisp":"1987-","dateIssuedKey":"1987","publisherPlace":"New York, NY [u.a.]","publisher":"Elsevier"}],"disp":"Human glioblastoma stem-like cells accumulate protoporphyrin IX when subjected to exogenous 5-aminolaevulinic acid, rendering them sensitive to photodynamic treatmentJournal of photochemistry and photobiology. B, Biology","type":{"media":"Online-Ressource","bibl":"periodical"},"recId":"300591519","id":{"issn":["1873-2682"],"zdb":["1482691-4"],"eki":["300591519"]},"title":[{"partname":"Biology","title":"Journal of photochemistry and photobiology","title_sort":"Journal of photochemistry and photobiology","subtitle":"official journal of the European Society for Photobiology"}],"physDesc":[{"extent":"Online-Ressource"}],"part":{"extent":"8","text":"163(2016), Seite 203-210","year":"2016","pages":"203-210","volume":"163"}}],"physDesc":[{"extent":"8 S."}],"title":[{"title":"Human glioblastoma stem-like cells accumulate protoporphyrin IX when subjected to exogenous 5-aminolaevulinic acid, rendering them sensitive to photodynamic treatment","title_sort":"Human glioblastoma stem-like cells accumulate protoporphyrin IX when subjected to exogenous 5-aminolaevulinic acid, rendering them sensitive to photodynamic treatment"}]} | ||
| SRT | |a SCHIMANSKIHUMANGLIOB2016 | ||