Transient expression of intron-containing transgenes generates non-spliced aberrant pre-mRNAs that are processed into siRNAs
Main conclusionIn this study, we show that aberrant pre-mRNAs from non-spliced and non-polyadenylated intron-containing transgenes are channelled to the RNA silencing pathway.In plants, improperly processed transcripts are called aberrant RNAs (ab-RNAs) and are eliminated by either RNA silencing or...
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2019
|
| In: |
Planta
Year: 2019, Jahrgang: 249, Heft: 2, Pages: 457-468 |
| ISSN: | 1432-2048 |
| DOI: | 10.1007/s00425-018-3015-6 |
| Online-Zugang: | Verlag, Volltext: https://doi.org/10.1007/s00425-018-3015-6
|
| Verfasserangaben: | Athanasios Dalakouras, Anja Lauter, Alexandra Bassler, Gabi Krczal, Michael Wassenegger |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1669555208 | ||
| 003 | DE-627 | ||
| 005 | 20220816194559.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 190722s2019 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s00425-018-3015-6 |2 doi | |
| 035 | |a (DE-627)1669555208 | ||
| 035 | |a (DE-599)KXP1669555208 | ||
| 035 | |a (OCoLC)1341233956 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Dalakouras, Athanasios |e VerfasserIn |0 (DE-588)143167553 |0 (DE-627)704409313 |0 (DE-576)335448240 |4 aut | |
| 245 | 1 | 0 | |a Transient expression of intron-containing transgenes generates non-spliced aberrant pre-mRNAs that are processed into siRNAs |c Athanasios Dalakouras, Anja Lauter, Alexandra Bassler, Gabi Krczal, Michael Wassenegger |
| 264 | 1 | |c 2019 | |
| 300 | |a 12 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Published online: 24 September 2018 | ||
| 500 | |a Gesehen am 22.07.2019 | ||
| 520 | |a Main conclusionIn this study, we show that aberrant pre-mRNAs from non-spliced and non-polyadenylated intron-containing transgenes are channelled to the RNA silencing pathway.In plants, improperly processed transcripts are called aberrant RNAs (ab-RNAs) and are eliminated by either RNA silencing or RNA decay mechanisms. Ab-RNAs transcribed from intronless genes are copied by RNA-directed RNA polymerases (RDRs) into double-stranded RNAs which are subsequently cleaved by DICER-LIKE endonucleases into small RNAs (sRNAs). In contrast, ab-RNAs from intron-containing genes are suggested to be channelled post-splicing to exonucleolytic degradation. Yet, it is not clear how non-spliced aberrant pre-mRNAs are eliminated. We reasoned that transient expression of agroinfiltrated intron-containing transgenes in Nicotiana benthamiana would allow us to study the steady-state levels of non-spliced pre-mRNAs. SRNA deep sequencing of the agroinfiltrated transgenes revealed the presence of sRNAs mapping to the entire non-spliced pre-mRNA suggesting that RDRs (most likely RDR6) processed aberrant non-spliced pre-mRNAs. Primary and secondary sRNAs with lengths of 18-25 nucleotides (nt) were detected, with the most prominent sRNA size class of 22 nt. SRNAs also mapped to the terminator sequence, indicating that RDR substrates also comprised read-through transcripts devoid of polyadenylation tail. Importantly, the occurring sRNAs efficiently targeted cognate mRNA for degradation but failed to cleave the non-spliced pre-mRNA, corroborating the notion that sRNAs are not triggering RNA cleavage in the nucleus. | ||
| 650 | 4 | |a DCLs | |
| 650 | 4 | |a Intron | |
| 650 | 4 | |a Nicotiana benthamiana | |
| 650 | 4 | |a RDRs | |
| 650 | 4 | |a RNA silencing | |
| 650 | 4 | |a Small RNAs | |
| 650 | 4 | |a Splicing | |
| 650 | 4 | |a Transgene | |
| 700 | 1 | |a Wassenegger, Michael |e VerfasserIn |0 (DE-588)105844929X |0 (DE-627)796896194 |0 (DE-576)414628934 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Planta |d Berlin : Springer, 1925 |g 249(2019), 2, Seite 457-468 |h Online-Ressource |w (DE-627)254639100 |w (DE-600)1463030-8 |w (DE-576)074531786 |x 1432-2048 |7 nnas |a Transient expression of intron-containing transgenes generates non-spliced aberrant pre-mRNAs that are processed into siRNAs |
| 773 | 1 | 8 | |g volume:249 |g year:2019 |g number:2 |g pages:457-468 |g extent:12 |a Transient expression of intron-containing transgenes generates non-spliced aberrant pre-mRNAs that are processed into siRNAs |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00425-018-3015-6 |x Verlag |x Resolving-System |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20190722 | ||
| 993 | |a Article | ||
| 994 | |a 2019 | ||
| 998 | |g 105844929X |a Wassenegger, Michael |m 105844929X:Wassenegger, Michael |d 140000 |e 140000PW105844929X |k 0/140000/ |p 5 |y j | ||
| 999 | |a KXP-PPN1669555208 |e 3495504974 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"person":[{"role":"aut","display":"Dalakouras, Athanasios","roleDisplay":"VerfasserIn","given":"Athanasios","family":"Dalakouras"},{"given":"Michael","family":"Wassenegger","role":"aut","display":"Wassenegger, Michael","roleDisplay":"VerfasserIn"}],"title":[{"title":"Transient expression of intron-containing transgenes generates non-spliced aberrant pre-mRNAs that are processed into siRNAs","title_sort":"Transient expression of intron-containing transgenes generates non-spliced aberrant pre-mRNAs that are processed into siRNAs"}],"language":["eng"],"recId":"1669555208","type":{"media":"Online-Ressource","bibl":"article-journal"},"note":["Published online: 24 September 2018","Gesehen am 22.07.2019"],"name":{"displayForm":["Athanasios Dalakouras, Anja Lauter, Alexandra Bassler, Gabi Krczal, Michael Wassenegger"]},"id":{"eki":["1669555208"],"doi":["10.1007/s00425-018-3015-6"]},"origin":[{"dateIssuedDisp":"2019","dateIssuedKey":"2019"}],"relHost":[{"title":[{"title":"Planta","subtitle":"an international journal of plant biology","title_sort":"Planta"}],"part":{"text":"249(2019), 2, Seite 457-468","volume":"249","extent":"12","year":"2019","issue":"2","pages":"457-468"},"titleAlt":[{"title":"Archiv für wissenschaftliche Botanik"}],"pubHistory":["1.1925 -"],"recId":"254639100","language":["eng"],"note":["Gesehen am 09.10.2013"],"disp":"Transient expression of intron-containing transgenes generates non-spliced aberrant pre-mRNAs that are processed into siRNAsPlanta","type":{"media":"Online-Ressource","bibl":"periodical"},"id":{"zdb":["1463030-8"],"eki":["254639100"],"issn":["1432-2048"]},"origin":[{"publisher":"Springer ; Springer","dateIssuedKey":"1925","dateIssuedDisp":"1925-","publisherPlace":"Berlin ; Heidelberg ; Berlin ; Heidelberg ; New York, NY"}],"physDesc":[{"extent":"Online-Ressource"}]}],"physDesc":[{"extent":"12 S."}]} | ||
| SRT | |a DALAKOURASTRANSIENTE2019 | ||