Enhanced performance of next-generation sequencing diagnostics compared with standard of care microbiological diagnostics in patients suffering from septic shock
Objectives: Culture-based diagnostics represent the standard of care in septic patients, but are highly insensitive and in many cases unspecific. We recently demonstrated the general feasibility of next-generation sequencing-based diagnostics using free circulating nucleic acids (cell-free DNA) in p...
Gespeichert in:
| Hauptverfasser: | , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
May 2019
|
| In: |
Critical care medicine
Year: 2019, Jahrgang: 47, Heft: 5, Pages: e394-e402 |
| ISSN: | 1530-0293 |
| DOI: | 10.1097/CCM.0000000000003658 |
| Online-Zugang: | Verlag, Volltext: https://doi.org/10.1097/CCM.0000000000003658 Verlag, Volltext: https://journals.lww.com/ccmjournal/Fulltext/2019/05000/Enhanced_Performance_of_Next_Generation_Sequencing.21.aspx 
|
| Verfasserangaben: | Silke Grumaz, Christian Grumaz, Yevhen Vainshtein, Philip Stevens, Karolina Glanz, Sebastian O. Decker, Stefan Hofer, Markus A. Weigand, Thorsten Brenner, Kai Sohn |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1671184890 | ||
| 003 | DE-627 | ||
| 005 | 20240323101247.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 190812s2019 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1097/CCM.0000000000003658 |2 doi | |
| 035 | |a (DE-627)1671184890 | ||
| 035 | |a (DE-599)KXP1671184890 | ||
| 035 | |a (OCoLC)1341235698 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Grumaz, Silke |d 1983- |e VerfasserIn |0 (DE-588)1050187733 |0 (DE-627)783582277 |0 (DE-576)404402186 |4 aut | |
| 245 | 1 | 0 | |a Enhanced performance of next-generation sequencing diagnostics compared with standard of care microbiological diagnostics in patients suffering from septic shock |c Silke Grumaz, Christian Grumaz, Yevhen Vainshtein, Philip Stevens, Karolina Glanz, Sebastian O. Decker, Stefan Hofer, Markus A. Weigand, Thorsten Brenner, Kai Sohn |
| 264 | 1 | |c May 2019 | |
| 300 | |a 9 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 12.08.2019 | ||
| 520 | |a Objectives: Culture-based diagnostics represent the standard of care in septic patients, but are highly insensitive and in many cases unspecific. We recently demonstrated the general feasibility of next-generation sequencing-based diagnostics using free circulating nucleic acids (cell-free DNA) in plasma samples of septic patients. Within the presented investigation, higher performance of next-generation sequencing-based diagnostics was validated by comparison to matched blood cultures. - Design: A secondary analysis of a prospective, observational, single-center study. - Setting: Surgical ICU of a university hospital and research laboratory. - Patients: Fifty patients with septic shock, 20 uninfected patients with elective surgery as control cohort. - Interventions: None. - Measurements and Main Results: From 256 plasma samples of 48 septic patients at up to seven consecutive time points within the 28-day observation period, cell-free DNA was isolated and analyzed by next-generation sequencing and relevance scoring. In parallel, results from culture-based diagnostics (e.g., blood culture) were obtained. Plausibility of blood culture and next-generation sequencing results as well as adequacy of antibiotic therapy was evaluated by an independent expert panel. In contrast to blood culture with a positivity rate of 33% at sepsis onset, the positivity rate for next-generation sequencing-based pathogen identification was 72%. Over the whole study period, blood culture positivity was 11%, and next-generation sequencing positivity was 71%. Ninety-six percent of positive next-generation sequencing results for acute sepsis time points were plausible and would have led to a change to a more adequate therapy in 53% of cases as assessed by the expert evaluation. - Conclusions: Our results show that next-generation sequencing-based analyses of bloodstream infections provide a valuable diagnostic platform for the identification of clinically relevant pathogens with higher sensitivity and specificity than blood culture, indicating that patients might benefit from a more appropriate therapy based on next-generation sequencing-based diagnosis. | ||
| 700 | 1 | |a Decker, Sebastian |d 1984- |e VerfasserIn |0 (DE-588)139936041 |0 (DE-627)703511785 |0 (DE-576)313600880 |4 aut | |
| 700 | 1 | |a Weigand, Markus A. |d 1967- |e VerfasserIn |0 (DE-588)1064970435 |0 (DE-627)815375239 |0 (DE-576)424658666 |4 aut | |
| 700 | 1 | |a Brenner, Thorsten |d 1978- |e VerfasserIn |0 (DE-588)130125709 |0 (DE-627)491713363 |0 (DE-576)189360607 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Critical care medicine |d Hagerstown, Md. : Lippincott Williams & Wilkins, 1973 |g 47(2019), 5, Seite e394-e402 |h Online-Ressource |w (DE-627)325051682 |w (DE-600)2034247-0 |w (DE-576)094425582 |x 1530-0293 |7 nnas |a Enhanced performance of next-generation sequencing diagnostics compared with standard of care microbiological diagnostics in patients suffering from septic shock |
| 773 | 1 | 8 | |g volume:47 |g year:2019 |g number:5 |g pages:e394-e402 |g extent:9 |a Enhanced performance of next-generation sequencing diagnostics compared with standard of care microbiological diagnostics in patients suffering from septic shock |
| 856 | 4 | 0 | |u https://doi.org/10.1097/CCM.0000000000003658 |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u https://journals.lww.com/ccmjournal/Fulltext/2019/05000/Enhanced_Performance_of_Next_Generation_Sequencing.21.aspx |x Verlag |x Resolving-System |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20190812 | ||
| 993 | |a Article | ||
| 994 | |a 2019 | ||
| 998 | |g 130125709 |a Brenner, Thorsten |m 130125709:Brenner, Thorsten |d 910000 |d 910300 |d 50000 |e 910000PB130125709 |e 910300PB130125709 |e 50000PB130125709 |k 0/910000/ |k 1/910000/910300/ |k 0/50000/ |p 9 | ||
| 998 | |g 1064970435 |a Weigand, Markus A. |m 1064970435:Weigand, Markus A. |d 910000 |d 910300 |e 910000PW1064970435 |e 910300PW1064970435 |k 0/910000/ |k 1/910000/910300/ |p 8 | ||
| 998 | |g 139936041 |a Decker, Sebastian |m 139936041:Decker, Sebastian |d 910000 |d 910300 |e 910000PD139936041 |e 910300PD139936041 |k 0/910000/ |k 1/910000/910300/ |p 6 | ||
| 999 | |a KXP-PPN1671184890 |e 3506425781 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"language":["eng"],"title":[{"title":"Enhanced performance of next-generation sequencing diagnostics compared with standard of care microbiological diagnostics in patients suffering from septic shock","title_sort":"Enhanced performance of next-generation sequencing diagnostics compared with standard of care microbiological diagnostics in patients suffering from septic shock"}],"recId":"1671184890","origin":[{"dateIssuedKey":"2019","dateIssuedDisp":"May 2019"}],"name":{"displayForm":["Silke Grumaz, Christian Grumaz, Yevhen Vainshtein, Philip Stevens, Karolina Glanz, Sebastian O. Decker, Stefan Hofer, Markus A. Weigand, Thorsten Brenner, Kai Sohn"]},"person":[{"role":"aut","family":"Grumaz","given":"Silke","display":"Grumaz, Silke"},{"family":"Decker","given":"Sebastian","display":"Decker, Sebastian","role":"aut"},{"role":"aut","display":"Weigand, Markus A.","given":"Markus A.","family":"Weigand"},{"role":"aut","family":"Brenner","display":"Brenner, Thorsten","given":"Thorsten"}],"relHost":[{"note":["Gesehen am 20.05.19","Fortsetzung der Druck-Ausgabe"],"id":{"issn":["1530-0293"],"zdb":["2034247-0"],"eki":["325051682"]},"part":{"issue":"5","extent":"9","pages":"e394-e402","year":"2019","text":"47(2019), 5, Seite e394-e402","volume":"47"},"physDesc":[{"extent":"Online-Ressource"}],"title":[{"subtitle":"the official journal of the Society of Critical Care Medicine","title_sort":"Critical care medicine","title":"Critical care medicine"}],"origin":[{"dateIssuedKey":"1973","publisherPlace":"Hagerstown, Md. ; [Erscheinungsort nicht ermittelbar]","publisher":"Lippincott Williams & Wilkins ; Ovid","dateIssuedDisp":"1973-"}],"corporate":[{"role":"isb","display":"Society of Critical Care Medicine"}],"pubHistory":["1.1973 -"],"type":{"media":"Online-Ressource","bibl":"periodical"},"language":["eng"],"disp":"Enhanced performance of next-generation sequencing diagnostics compared with standard of care microbiological diagnostics in patients suffering from septic shockCritical care medicine","recId":"325051682"}],"note":["Gesehen am 12.08.2019"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"id":{"doi":["10.1097/CCM.0000000000003658"],"eki":["1671184890"]},"physDesc":[{"extent":"9 S."}]} | ||
| SRT | |a GRUMAZSILKENHANCEDPE2019 | ||