Buchumschlag

Programmable functionalization of surfactant-stabilized microfluidic droplets via DNA-tags

Droplet-based microfluidics has emerged as a powerful tool in synthetic biology. For many applications, chemical functionalization of the droplets is a key process. Therefore, a straightforward and broadly applicable approach is developed to functionalize the inner periphery of microfluidic droplets...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Jahnke, Kevin (VerfasserIn) , Weiss, Marian (VerfasserIn) , Frey, Christoph (VerfasserIn) , Antona, Silvia (VerfasserIn) , Janiesch, Jan-Willi (VerfasserIn) , Platzman, Ilia (VerfasserIn) , Göpfrich, Kerstin (VerfasserIn) , Spatz, Joachim P. (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: April 8, 2019
In: Advanced functional materials
Year: 2019, Jahrgang: 29, Heft: 23, Pages: 1-8
ISSN:1616-3028
DOI:10.1002/adfm.201808647
Online-Zugang:Verlag, Volltext: https://doi.org/10.1002/adfm.201808647
Verlag, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/adfm.201808647
Volltext
Verfasserangaben:Kevin Jahnke, Marian Weiss, Christoph Frey, Silvia Antona, Jan-Willi Janiesch, Ilia Platzman, Kerstin Göpfrich, and Joachim P. Spatz
Beschreibung
Zusammenfassung:Droplet-based microfluidics has emerged as a powerful tool in synthetic biology. For many applications, chemical functionalization of the droplets is a key process. Therefore, a straightforward and broadly applicable approach is developed to functionalize the inner periphery of microfluidic droplets with diverse reactive groups and components. Instead of covalent modification of the droplet-stabilizing surfactants, this method relies on cholesterol-tagged DNA that self-assembles at the droplet periphery. The cholesterol-tagged DNA serves as an attachment handle for the recruitment of complementary DNA. The complementary DNA can carry diverse functional groups. We exemplify our method by demonstrating the attachment of amine groups, DNA nanostructures, microspheres, a minimal actin cortex, and leukemia cells to the droplet periphery. It is further shown that the DNA-mediated attachment to the droplet periphery is temperature-responsive and reversible. It is envisioned that droplet functionalization via DNA handles will help to tailor droplet interfaces for diverse applications—featuring programmable assembly, unique addressability, and stimuli-responsiveness.
Beschreibung:Gesehen am 12.08.2019
Beschreibung:Online Resource
ISSN:1616-3028
DOI:10.1002/adfm.201808647

Ähnliche Einträge