Live cell imaging of cytoplasmic and nuclear Ca2+ dynamics in Arabidopsis roots
Approaches for Ca(2+) measurement require data recording with high spatiotemporal resolution. This is achieved by combined usage of locally targeted genetically encoded calcium indicators (GECIs) and confocal laser scanning microscopy (CLSM). This protocol provides instructions for setting up a CLSM...
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| Hauptverfasser: | , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2013 Aug 1
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| In: |
Cold Spring Harbor protocols
Year: 2013, Heft: 8, Pages: 776-780 |
| ISSN: | 1559-6095 |
| Online-Zugang: |
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| Verfasserangaben: | Melanie Krebs, Karin Schumacher |
| Zusammenfassung: | Approaches for Ca(2+) measurement require data recording with high spatiotemporal resolution. This is achieved by combined usage of locally targeted genetically encoded calcium indicators (GECIs) and confocal laser scanning microscopy (CLSM). This protocol provides instructions for setting up a CLSM-based experiment to measure cytosolic and nuclear Ca(2+) dynamics in Arabidopsis lines expressing Yellow Cameleon Ca(2+) indicators. |
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| Beschreibung: | Im Titel ist „2+“ hochgestellt Gesehen am 14.08.2019 |
| Beschreibung: | Online Resource |
| ISSN: | 1559-6095 |