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Lectinology 4.0: altering modular (ga)lectin display for functional analysis and biomedical applications

BACKGROUND: Recognition of glycans by lectins is emerging as (patho)physiologically broadly used mode of cellular information transfer. Whereas the direct ligand-receptor contact is often already thoroughly characterized, the functional relevance of aspects of architecture such as modular design and...

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Hauptverfasser: Ludwig, Anna-Kristin (VerfasserIn) , Kaltner, Herbert (VerfasserIn) , Kopitz, Jürgen (VerfasserIn) , Gabius, Hans-Joachim (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 6 March 2019
In: Biochimica et biophysica acta. Specialized section on biophysical subjects
Year: 2019, Jahrgang: 1863, Heft: 5, Pages: 935-940
ISSN:0926-6577
DOI:10.1016/j.bbagen.2019.03.005
Online-Zugang:Verlag, Volltext: https://doi.org/10.1016/j.bbagen.2019.03.005
Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S0304416519300558
Volltext
Verfasserangaben:Anna-Kristin Ludwig, Herbert Kaltner, Jürgen Kopitz, Hans-Joachim Gabius
Beschreibung
Zusammenfassung:BACKGROUND: Recognition of glycans by lectins is emerging as (patho)physiologically broadly used mode of cellular information transfer. Whereas the direct ligand-receptor contact is often already thoroughly characterized, the functional relevance of aspects of architecture such as modular design and valence of lectins is less well defined. - SCOPE OF REVIEW: Following an introduction to modular lectin design, three levels of methodology are then reviewed that delineate lectin structure-activity relationships beyond glycan binding, with emphasis on domain shuffling. - MAJOR CONCLUSIONS: Engineering of variants by modular transplantation facilitates versatile Nature-inspired design switches and access to new combinations with translational potential, as exemplified for human adhesion/growth-regulatory galectins. - GENERAL SIGNIFICANCE: To gain an understanding of the functional significance of natural variations in quaternary structure and modular design within a protein family is a current challenge. Strategic application of methods of the described phases is a means to respond to this challenge.
Beschreibung:Gesehen am 09.09.2019
Beschreibung:Online Resource
ISSN:0926-6577
DOI:10.1016/j.bbagen.2019.03.005

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