Hypovitaminosis D upscales B-cell immunoreactivity in multiple sclerosis
Background - While vitamin D is increasingly recognized as a potential immune regulator of MS disease activity, its impact on B lymphocytes, however, remains ill-defined. - Methods - We assessed the impact of vitamin D on B-cell proliferation and cytokine secretion ex vivo and screened for effects o...
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| Main Authors: | , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
23 March 2016
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| In: |
Journal of neuroimmunology
Year: 2016, Volume: 294, Pages: 18-26 |
| ISSN: | 1872-8421 |
| DOI: | 10.1016/j.jneuroim.2016.03.011 |
| Online Access: | Verlag, Volltext: https://doi.org/10.1016/j.jneuroim.2016.03.011 Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S0165572816300455 
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| Author Notes: | Jürgen Haas, Alexander Schwarz, Mirjam Korporal-Kuhnke, Simon Faller, Sven Jarius, Brigitte Wildemann |
| Summary: | Background - While vitamin D is increasingly recognized as a potential immune regulator of MS disease activity, its impact on B lymphocytes, however, remains ill-defined. - Methods - We assessed the impact of vitamin D on B-cell proliferation and cytokine secretion ex vivo and screened for effects of hypovitaminosis D and vitamin D supplementation on the compartmentalized distribution of B-cell subtypes in peripheral blood and cerebrospinal fluid (CSF) from patients with relapsing remitting MS (n=95) and various neurologic and healthy controls (n=57). - Results - B cells from MS patients with 25(OH)D serum levels <20ng/ml, displayed enhanced immunoreactivity ex vivo as a consequence of more vigorous responses of CD27+ memory phenotypes. Immune responses decreased when B cells from either source were co-cultured in the presence of vitamin D or when retesting B cells from MS patients after prolonged supplementation with vitamin D. Hypovitaminosis D was detectable in the serum of 40/95 MS patients, correlated with decreased vitamin D concentrations in CSF and with higher disease activity, and was paralleled by intrathecal accumulation of CD27+ B-cell subtypes and plasma cells. - Conclusion - B-cell immunoreactivity is attenuated by vitamin D. Our finding that vitamin D deficiency affects the intrathecal compartment and coincides with increased frequencies of effector B-cell subtypes in the CSF suggests that hypovitaminosis D might contribute to augmenting disease activity in the target organ and supports a potential benefit of vitamin D supplementation in MS. |
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| Item Description: | Gesehen am 11.09.2019 |
| Physical Description: | Online Resource |
| ISSN: | 1872-8421 |
| DOI: | 10.1016/j.jneuroim.2016.03.011 |