Ursodeoxycholyl iysophosphatidylethanolamide negatively regulates TLR-mediated lipopolysaccharide response in human THP-1-derived macrophages
The bile acid-phospholipid conjugate ursodeoxycholyl oleoyl-lysophophatidylethanolamide (UDCA-18:1LPE) is an anti-inflammatory and anti-fibrotic agent as previously shown in cultured hepatocytes and hepatic stellate cells as well as in in vivo models of liver injury. We hypothesize that UDCA-18:1LPE...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
20 February 2018
|
| In: |
European journal of pharmacology
Year: 2018, Volume: 825, Pages: 63-74 |
| ISSN: | 1879-0712 |
| DOI: | 10.1016/j.ejphar.2018.02.030 |
| Online Access: | Verlag, Volltext: https://doi.org/10.1016/j.ejphar.2018.02.030 Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S0014299918301079 
|
| Author Notes: | Alzbeta Horvatova, Tanyarath Utaipan, Ann-Christin Otto, Yuling Zhang, Hongying Gan-Schreier, Petr Pavek, Anita Pathil, Wolfgang Stremmel, Walee Chamulitrat |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1676568735 | ||
| 003 | DE-627 | ||
| 005 | 20220816223249.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 190912s2018 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.ejphar.2018.02.030 |2 doi | |
| 035 | |a (DE-627)1676568735 | ||
| 035 | |a (DE-599)KXP1676568735 | ||
| 035 | |a (OCoLC)1341243171 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Horvatova, Alzbeta |e VerfasserIn |0 (DE-588)1194552056 |0 (DE-627)1676568077 |4 aut | |
| 245 | 1 | 0 | |a Ursodeoxycholyl iysophosphatidylethanolamide negatively regulates TLR-mediated lipopolysaccharide response in human THP-1-derived macrophages |c Alzbeta Horvatova, Tanyarath Utaipan, Ann-Christin Otto, Yuling Zhang, Hongying Gan-Schreier, Petr Pavek, Anita Pathil, Wolfgang Stremmel, Walee Chamulitrat |
| 264 | 1 | |c 20 February 2018 | |
| 300 | |a 12 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 12.09.2019 | ||
| 520 | |a The bile acid-phospholipid conjugate ursodeoxycholyl oleoyl-lysophophatidylethanolamide (UDCA-18:1LPE) is an anti-inflammatory and anti-fibrotic agent as previously shown in cultured hepatocytes and hepatic stellate cells as well as in in vivo models of liver injury. We hypothesize that UDCA-18:1LPE may directly inhibit the activation of immune cells. We found that UDCA-18:1LPE was capable of inhibiting the migration of phorbol ester-differentiated human THP-1 cells. We examined anti-inflammatory activity of UDCA-18:1LPE during activation of THP1-derived macrophages. Treatment of these macrophages by bacterial lipopolysaccharide (LPS) for 24h induced the release of pro-inflammatory cytokines TNF-α, IL-6 and IL-1β. This release was markedly inhibited by pretreatment with UDCA-18:1LPE by ~ 65-90%. Derivatives with a different fatty-acid chain in LPE moiety also exhibited anti-inflammatory property. Western blotting and indirect immunofluorescence analyses revealed that UDCA-18:1LPE attenuated the expression of phosphorylated p38, MKK4/MKK7, JNK1/2, and c-Jun as well as nuclear translocation of NF-κB by ~ 22-86%. After LPS stimulation, the Toll-like receptor adaptor proteins, myeloid differentiation factor 88 and TNF receptor associated factor 6, were recruited into lipid rafts and UDCA-18:1LPE inhibited this recruitment by 22% and 58%, respectively. Moreover, LPS treatment caused a decrease of the known cytoprotective lysophosphatidylcholine species containing polyunsaturated fatty acids by 43%, and UDCA-18:1LPE co-treatment reversed this decrease. In conclusion, UDCA-18:1LPE and derivatives inhibited LPS inflammatory response by interfering with Toll-like receptor signaling in lipid rafts leading to an inhibition of MAPK and NF-κB activation. These conjugates may represent a class of lead compounds for development of anti-inflammatory drugs. | ||
| 650 | 4 | |a Bile-acid and phospholipid conjugates | |
| 650 | 4 | |a Cytokines | |
| 650 | 4 | |a Lipid rafts | |
| 650 | 4 | |a Lipopolysaccharides | |
| 650 | 4 | |a Macrophages | |
| 650 | 4 | |a Signaling pathways | |
| 700 | 1 | |a Otto, Ann-Christin |e VerfasserIn |0 (DE-588)1193099277 |0 (DE-627)1671656180 |4 aut | |
| 700 | 1 | |a Zhang, Yuling |d 1988- |e VerfasserIn |0 (DE-588)1194552102 |0 (DE-627)1676568247 |4 aut | |
| 700 | 1 | |a Gan-Schreier, Hongying |d 1967- |e VerfasserIn |0 (DE-588)130102040 |0 (DE-627)490859860 |0 (DE-576)297997955 |4 aut | |
| 700 | 1 | |a Pathil-Warth, Anita |d 1981- |e VerfasserIn |0 (DE-588)132644304 |0 (DE-627)524715890 |0 (DE-576)260118915 |4 aut | |
| 700 | 1 | |a Stremmel, Wolfgang |d 1952- |e VerfasserIn |0 (DE-588)142773638 |0 (DE-627)640118747 |0 (DE-576)33331560X |4 aut | |
| 700 | 1 | |a Chamulitrat, Walee |d 1959- |e VerfasserIn |0 (DE-588)1037336828 |0 (DE-627)755493915 |0 (DE-576)39134479X |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t European journal of pharmacology |d New York, NY [u.a.] : Elsevier, 1967 |g 825(2018), Seite 63-74 |h Online-Ressource |w (DE-627)300897340 |w (DE-600)1483526-5 |w (DE-576)081952627 |x 1879-0712 |7 nnas |a Ursodeoxycholyl iysophosphatidylethanolamide negatively regulates TLR-mediated lipopolysaccharide response in human THP-1-derived macrophages |
| 773 | 1 | 8 | |g volume:825 |g year:2018 |g pages:63-74 |g extent:12 |a Ursodeoxycholyl iysophosphatidylethanolamide negatively regulates TLR-mediated lipopolysaccharide response in human THP-1-derived macrophages |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.ejphar.2018.02.030 |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u http://www.sciencedirect.com/science/article/pii/S0014299918301079 |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20190912 | ||
| 994 | |a 2018 | ||
| 998 | |g 1037336828 |a Chamulitrat, Walee |m 1037336828:Chamulitrat, Walee |d 910000 |d 910100 |e 910000PC1037336828 |e 910100PC1037336828 |k 0/910000/ |k 1/910000/910100/ |p 9 |y j | ||
| 998 | |g 142773638 |a Stremmel, Wolfgang |m 142773638:Stremmel, Wolfgang |d 910000 |d 910100 |e 910000PS142773638 |e 910100PS142773638 |k 0/910000/ |k 1/910000/910100/ |p 8 | ||
| 998 | |g 132644304 |a Pathil-Warth, Anita |m 132644304:Pathil-Warth, Anita |d 910000 |d 910100 |e 910000PP132644304 |e 910100PP132644304 |k 0/910000/ |k 1/910000/910100/ |p 7 | ||
| 998 | |g 130102040 |a Gan-Schreier, Hongying |m 130102040:Gan-Schreier, Hongying |d 910000 |d 910100 |e 910000PG130102040 |e 910100PG130102040 |k 0/910000/ |k 1/910000/910100/ |p 5 | ||
| 998 | |g 1194552102 |a Zhang, Yuling |m 1194552102:Zhang, Yuling |d 910000 |d 910100 |e 910000PZ1194552102 |e 910100PZ1194552102 |k 0/910000/ |k 1/910000/910100/ |p 4 | ||
| 998 | |g 1193099277 |a Otto, Ann-Christin |m 1193099277:Otto, Ann-Christin |d 50000 |e 50000PO1193099277 |k 0/50000/ |p 3 | ||
| 998 | |g 1194552056 |a Horvatova, Alzbeta |m 1194552056:Horvatova, Alzbeta |d 910000 |d 910100 |e 910000PH1194552056 |e 910100PH1194552056 |k 0/910000/ |k 1/910000/910100/ |p 1 |x j | ||
| 999 | |a KXP-PPN1676568735 |e 3514650446 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"name":{"displayForm":["Alzbeta Horvatova, Tanyarath Utaipan, Ann-Christin Otto, Yuling Zhang, Hongying Gan-Schreier, Petr Pavek, Anita Pathil, Wolfgang Stremmel, Walee Chamulitrat"]},"person":[{"display":"Horvatova, Alzbeta","given":"Alzbeta","family":"Horvatova","roleDisplay":"VerfasserIn","role":"aut"},{"family":"Otto","given":"Ann-Christin","display":"Otto, Ann-Christin","roleDisplay":"VerfasserIn","role":"aut"},{"roleDisplay":"VerfasserIn","role":"aut","display":"Zhang, Yuling","given":"Yuling","family":"Zhang"},{"given":"Hongying","family":"Gan-Schreier","display":"Gan-Schreier, Hongying","role":"aut","roleDisplay":"VerfasserIn"},{"display":"Pathil-Warth, Anita","given":"Anita","family":"Pathil-Warth","roleDisplay":"VerfasserIn","role":"aut"},{"display":"Stremmel, Wolfgang","given":"Wolfgang","family":"Stremmel","role":"aut","roleDisplay":"VerfasserIn"},{"roleDisplay":"VerfasserIn","role":"aut","family":"Chamulitrat","given":"Walee","display":"Chamulitrat, Walee"}],"physDesc":[{"extent":"12 S."}],"title":[{"title_sort":"Ursodeoxycholyl iysophosphatidylethanolamide negatively regulates TLR-mediated lipopolysaccharide response in human THP-1-derived macrophages","title":"Ursodeoxycholyl iysophosphatidylethanolamide negatively regulates TLR-mediated lipopolysaccharide response in human THP-1-derived macrophages"}],"language":["eng"],"note":["Gesehen am 12.09.2019"],"recId":"1676568735","type":{"media":"Online-Ressource","bibl":"article-journal"},"relHost":[{"physDesc":[{"extent":"Online-Ressource"}],"id":{"eki":["300897340"],"issn":["1879-0712"],"zdb":["1483526-5"]},"titleAlt":[{"title":"EJP"}],"origin":[{"dateIssuedDisp":"1967-","publisher":"Elsevier","dateIssuedKey":"1967","publisherPlace":"New York, NY [u.a.]"}],"disp":"Ursodeoxycholyl iysophosphatidylethanolamide negatively regulates TLR-mediated lipopolysaccharide response in human THP-1-derived macrophagesEuropean journal of pharmacology","type":{"media":"Online-Ressource","bibl":"periodical"},"recId":"300897340","part":{"text":"825(2018), Seite 63-74","extent":"12","volume":"825","pages":"63-74","year":"2018"},"language":["eng"],"pubHistory":["1.1967 -"],"title":[{"title_sort":"European journal of pharmacology","subtitle":"EJP","title":"European journal of pharmacology"}]}],"origin":[{"dateIssuedDisp":"20 February 2018","dateIssuedKey":"2018"}],"id":{"eki":["1676568735"],"doi":["10.1016/j.ejphar.2018.02.030"]}} | ||
| SRT | |a HORVATOVAAURSODEOXYC2020 | ||