Differential N-end rule degradation of RIN4/NOI fragments generated by the AvrRpt2 effector protease
In plants, the protein RPM1-INTERACTING PROTEIN4 (RIN4) is a central regulator of both pattern-triggered immunity and effector-triggered immunity. RIN4 is targeted by several effectors, including the Pseudomonas syringae protease effector AvrRpt2. Cleavage of RIN4 by AvrRpt2 generates potentially un...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
August 2019
|
| In: |
Plant physiology
Year: 2019, Volume: 180, Issue: 4, Pages: 2272-2289 |
| ISSN: | 1532-2548 |
| DOI: | 10.1104/pp.19.00251 |
| Online Access: | Verlag, Volltext: https://doi.org/10.1104/pp.19.00251 Verlag, Volltext: http://www.plantphysiol.org/content/180/4/2272 
|
| Author Notes: | Kevin Goslin, Lennart Eschen-Lippold, Christin Naumann, Eric Linster, Maud Sorel, Maria Klecker, Rémi de Marchi, Anne Kind, Markus Wirtz, Justin Lee, Nico Dissmeyer, Emmanuelle Graciet |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1677255471 | ||
| 003 | DE-627 | ||
| 005 | 20220816230252.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 190918s2019 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1104/pp.19.00251 |2 doi | |
| 035 | |a (DE-627)1677255471 | ||
| 035 | |a (DE-599)KXP1677255471 | ||
| 035 | |a (OCoLC)1341244230 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Goslin, Kevin |e VerfasserIn |0 (DE-588)1195078935 |0 (DE-627)1677253282 |4 aut | |
| 245 | 1 | 0 | |a Differential N-end rule degradation of RIN4/NOI fragments generated by the AvrRpt2 effector protease |c Kevin Goslin, Lennart Eschen-Lippold, Christin Naumann, Eric Linster, Maud Sorel, Maria Klecker, Rémi de Marchi, Anne Kind, Markus Wirtz, Justin Lee, Nico Dissmeyer, Emmanuelle Graciet |
| 264 | 1 | |c August 2019 | |
| 300 | |a 18 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 18.09.2019 | ||
| 520 | |a In plants, the protein RPM1-INTERACTING PROTEIN4 (RIN4) is a central regulator of both pattern-triggered immunity and effector-triggered immunity. RIN4 is targeted by several effectors, including the Pseudomonas syringae protease effector AvrRpt2. Cleavage of RIN4 by AvrRpt2 generates potentially unstable RIN4 fragments, whose degradation leads to the activation of the resistance protein RESISTANT TO P. SYRINGAE2. Hence, identifying the determinants of RIN4 degradation is key to understanding RESISTANT TO P. SYRINGAE2-mediated effector-triggered immunity, as well as virulence functions of AvrRpt2. In addition to RIN4, AvrRpt2 cleaves host proteins from the nitrate-induced (NOI) domain family. Although cleavage of NOI domain proteins by AvrRpt2 may contribute to pattern-triggered immunity regulation, the (in)stability of these proteolytic fragments and the determinants regulating their stability remain unexamined. Notably, a common feature of RIN4, and of many NOI domain protein fragments generated by AvrRpt2 cleavage, is the exposure of a new N-terminal residue that is destabilizing according to the N-end rule. Using antibodies raised against endogenous RIN4, we show that the destabilization of AvrRpt2-cleaved RIN4 fragments is independent of the N-end rule pathway (recently renamed the N-degron pathway). By contrast, several NOI domain protein fragments are genuine substrates of the N-degron pathway. The discovery of this set of substrates considerably expands the number of known proteins targeted for degradation by this ubiquitin-dependent pathway in plants. These results advance our current understanding of the role of AvrRpt2 in promoting bacterial virulence. | ||
| 700 | 1 | |a Naumann, Christin |e VerfasserIn |0 (DE-588)1195080417 |0 (DE-627)1677254750 |4 aut | |
| 700 | 1 | |a Linster, Eric |e VerfasserIn |0 (DE-588)1060573687 |0 (DE-627)800328604 |0 (DE-576)416725953 |4 aut | |
| 700 | 1 | |a Wirtz, Markus |e VerfasserIn |0 (DE-588)1023118378 |0 (DE-627)717709353 |0 (DE-576)36641979X |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Plant physiology |d Oxford : Oxford University Press, 1926 |g 180(2019), 4, Seite 2272-2289 |h Online-Ressource |w (DE-627)314092579 |w (DE-600)2004346-6 |w (DE-576)090885635 |x 1532-2548 |7 nnas |a Differential N-end rule degradation of RIN4/NOI fragments generated by the AvrRpt2 effector protease |
| 773 | 1 | 8 | |g volume:180 |g year:2019 |g number:4 |g pages:2272-2289 |g extent:18 |a Differential N-end rule degradation of RIN4/NOI fragments generated by the AvrRpt2 effector protease |
| 856 | 4 | 0 | |u https://doi.org/10.1104/pp.19.00251 |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u http://www.plantphysiol.org/content/180/4/2272 |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20190918 | ||
| 993 | |a Article | ||
| 994 | |a 2019 | ||
| 998 | |g 1023118378 |a Wirtz, Markus |m 1023118378:Wirtz, Markus |d 700000 |d 721000 |e 700000PW1023118378 |e 721000PW1023118378 |k 0/700000/ |k 1/700000/721000/ |p 9 | ||
| 998 | |g 1060573687 |a Linster, Eric |m 1060573687:Linster, Eric |d 700000 |d 721000 |e 700000PL1060573687 |e 721000PL1060573687 |k 0/700000/ |k 1/700000/721000/ |p 4 | ||
| 998 | |g 1195080417 |a Naumann, Christin |m 1195080417:Naumann, Christin |d 700000 |d 721000 |e 700000PN1195080417 |e 721000PN1195080417 |k 0/700000/ |k 1/700000/721000/ |p 3 | ||
| 999 | |a KXP-PPN1677255471 |e 3516479779 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"relHost":[{"title":[{"title":"Plant physiology","title_sort":"Plant physiology"}],"type":{"media":"Online-Ressource","bibl":"periodical"},"corporate":[{"display":"American Society of Plant Physiologists","role":"isb"},{"display":"American Society of Plant Biologists","role":"isb"}],"language":["eng"],"physDesc":[{"extent":"Online-Ressource"}],"id":{"eki":["314092579"],"issn":["1532-2548"],"zdb":["2004346-6"]},"origin":[{"publisherPlace":"Oxford ; Rockville, Md.","dateIssuedKey":"1926","publisher":"Oxford University Press ; Soc.","dateIssuedDisp":"1926-"}],"name":{"displayForm":["publ. monthly by the American Society of Plant Biologists"]},"recId":"314092579","disp":"Differential N-end rule degradation of RIN4/NOI fragments generated by the AvrRpt2 effector proteasePlant physiology","titleAlt":[{"title":"A journal devoted to all phases of plant physiology"},{"title":"Volume 80, number 1 (January 1986)-volume 122, number 4 (April 2000): An international journal devoted to physiology, biochemistry, cellular and molecular biology, biophysics and environmental biology of plants"},{"title":"Volume 123, number 1 (May 2000)-volume 128, number 3 (March 2002): An international journal devoted to development, cellular and molecular biology, biochemistry, biophysics, physiology, environmental biology, molecular evolution, genomics, ecophysiology, and whole plant"},{"title":"Volume 128, number 4 (April 2002)-volume 169, number 2 (October 2015): An international journal devoted to basic research into how plants function, ranging from the molecular to the cellular to the whole plant levels, and including the interactions of plants with their biotic and abiotic environments"}],"note":["Fortsetzung der Druck-Ausgabe","Gesehen am 09.08.2022","Beteil. Körp. anfangs: American Society of Plant Physiologists"],"pubHistory":["1.1926 -"],"part":{"issue":"4","year":"2019","text":"180(2019), 4, Seite 2272-2289","extent":"18","pages":"2272-2289","volume":"180"}}],"note":["Gesehen am 18.09.2019"],"id":{"doi":["10.1104/pp.19.00251"],"eki":["1677255471"]},"origin":[{"dateIssuedDisp":"August 2019","dateIssuedKey":"2019"}],"name":{"displayForm":["Kevin Goslin, Lennart Eschen-Lippold, Christin Naumann, Eric Linster, Maud Sorel, Maria Klecker, Rémi de Marchi, Anne Kind, Markus Wirtz, Justin Lee, Nico Dissmeyer, Emmanuelle Graciet"]},"recId":"1677255471","person":[{"display":"Goslin, Kevin","role":"aut","given":"Kevin","family":"Goslin"},{"family":"Naumann","given":"Christin","role":"aut","display":"Naumann, Christin"},{"family":"Linster","role":"aut","given":"Eric","display":"Linster, Eric"},{"family":"Wirtz","given":"Markus","role":"aut","display":"Wirtz, Markus"}],"title":[{"title":"Differential N-end rule degradation of RIN4/NOI fragments generated by the AvrRpt2 effector protease","title_sort":"Differential N-end rule degradation of RIN4/NOI fragments generated by the AvrRpt2 effector protease"}],"type":{"media":"Online-Ressource","bibl":"article-journal"},"language":["eng"],"physDesc":[{"extent":"18 S."}]} | ||
| SRT | |a GOSLINKEVIDIFFERENTI2019 | ||